GP142 Level of 25(OH)-vitamin D and vitamin D receptor (VDR) gene polymorphisms in adolescents with chronic gastroduodenitis

Abstract

Introduction Chronic gastroduodenitis in adolescents is often accompanied by a decrease in bone density. The mechanisms of this relationship are not fully understood. Objective To study the level of 25 (OH)-vitamin D, vitamin D receptor gene polymorphisms and bone metabolism in adolescents with chronic gastroduodenitis in dependency of helicobacter pylori (HP) infection. Materials and methods 51 adolescents aged from 12 to 15 years old were examined. All patients had a morphologically checked chronic gastroduodenitis condition.Group 1 included 19 patients with HP infection and other 32 children without HP infection were put in a group 2. All children had a gastroenterological examination including urease test and taking a biopsy sample from the stomach. 25 (OH)-vitamin D in serum was determined by ELISA. Polymorphisms (ApaI, TagI, BsmI) of vitamin D receptor gene (VDR) were determined by PCR with restriction digest. Dual-energy x-ray absorptiometry of the lumbar region of the spine L1-L4 was carried out in all patients on a Hologic QDR 4500C densitometer equipped with pediatric reference base in order to study osteocalcin (OK) and b-Cross-Laps. Results A 25 (OH)-vitamin D deficiency was observed with an equal frequency in both groups (5.26% and 6.25%, p>0.05). The correlation between vitamin D levels and the presence of HP-infection was not found (r s = 0.417, p & gt; 0.05). At the same time, the frequency of occurrence of genotypes containing the allele B of the VDR BsmI gene was higher among patients without HP infection compared with children with Hp infection (p & lt; 0.05 c2 = 6.03). In both groups, the carriers of the VDR BsmI gene B had a significantly higher level of osteocalcin in comparison with children without it (90.43 ± 36.03 ng/ml and63.02 ± 34.12 ng/ml, p<0.005). The carriers of the ApaI gene A allele in both groups had high bone mineral density and high levels of 25 (OH)-vitamin D, osteocalcin, and b-cross-laps in comparison to children without this allele. Children with hp-infection, carriers of the t + genotype of the TagI gene of the VDR gene had a higher bone mineral density (p = 0.001). Conclusion In adolescents with chronic gastroduodenitis, the bone metabolism and the level of 25 (OH)-vitamin D are related to genetic polymorphism gene VDR, and the presence of HP-infection as well.