GRA2 and ROP1 Recombinant Antigens as Potential Markers for Detection of
            <i>Toxoplasma gondii</i>
            -Specific Immunoglobulin G in Humans with Acute Toxoplasmosis

Holec-Gąsior, Lucyna; Kur, Józef; Hiszczyńska-Sawicka, Elżbieta

doi:10.1128/cvi.00341-08

Cited by 49 publications

(42 citation statements)

References 25 publications

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“…Therefore, these proteins secreted from tachyzoites during their proliferation in the brain appear to stimulate production of antibody to these molecules. In humans, IgG antibodies to ROP1 and GRA2 are detected significantly more frequently in individuals with acute infection than in those with chronic infection (3,17). In addition, significantly One microliter of either rGRA2 (a fusion protein with pUET; 15.6 g/ml) or pUET solution (15.6 g/ml) was absorbed onto a nitrocellulose membrane.…”

Section: Discussionmentioning

confidence: 99%

Toxoplasma gondii Antigens Recognized by IgG Antibodies Differ between Mice with and without Active Proliferation of Tachyzoites in the Brain during the Chronic Stage of Infection

Hester

Mullins

et al. 2012

Infect Immun

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We examined whether tachyzoite proliferation in the brains of immunocompetent hosts during the chronic stage of infection with Toxoplasma gondii induces production of IgG antibodies that recognize parasite antigens different from those recognized by the antibodies of infected hosts that do not have tachyzoite growth. For this purpose, two groups of CBA/J mice, which display continuous tachyzoite growth in their brains during the later stage of infection, were infected, and one group received treatment with sulfadiazine to prevent tachyzoite proliferation during the chronic stage of infection. T. gondii antigens recognized by the IgG antibodies from these two groups of mice were compared using immunoblotting following separation of tachyzoite antigens by two-dimensional gel electrophoresis. Several antigens, including the microneme protein MIC2, the cyst matrix protein MAG1, and the dense granule proteins GRA4 and GRA7, were commonly recognized by IgG antibodies from both groups of mice. There were multiple antigens recognized mostly by IgG antibodies of only one group of mice, either with or without cerebral tachyzoite growth. The antigens recognized only by or mostly by the antibodies of mice with cerebral tachyzoite growth include MIC6, the rhoptry protein ROP1, GRA2, one heat shock protein HSP90, one (putative) HSP70, and the myosin heavy chain. These results indicate that levels of IgG antibody to only selected T. gondii antigens increase in association with cerebral tachyzoite proliferation (reactivation of infection) in immunocompetent hosts with chronic infection.

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Section: Discussionmentioning

confidence: 99%

Toxoplasma gondii Antigens Recognized by IgG Antibodies Differ between Mice with and without Active Proliferation of Tachyzoites in the Brain during the Chronic Stage of Infection

Hester

Mullins

et al. 2012

Infect Immun

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“…In the past 35 years, several dozen genes encoding T. gondii proteins have been cloned into bacterial and eukaryotic expression systems, as follows: the surface antigens SAG1 (P30) (9-12), SAG2 (P22) (12)(13)(14)(15), SAG3 (P43) (16), and P35 (15); the dense granule antigens GRA1 (P24) (11,17), GRA2 (P28) (18)(19)(20)(21)(22), GRA4 (23,24), GRA5 (25), GRA6 (P32) (15,26,27), and GRA7 (P29) (11,(28)(29)(30)(31); the rhoptry antigens ROP1 (P66) (20) and ROP2 (P54) (32)(33)(34); and B10 (P41) (35), MAG1 (36), and MIC1 (37). Many of these recombinant antigens have been used for the detection of T. gondii-specific antibodies in human serum samples (38).…”

Section: T Gondii Recombinant Antigensmentioning

confidence: 99%

Toxoplasma gondii Recombinant Antigens as Tools for Serodiagnosis of Human Toxoplasmosis: Current Status of Studies

Holec-Gąsior

2013

Clin Vaccine Immunol

113

105

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Toxoplasma gondii is a parasitic protozoan which is the cause of toxoplasmosis. Although human toxoplasmosis in healthy adults is usually asymptomatic, serious disease can occur in the case of congenital infections and immunocompromised individuals. Furthermore, despite the exact recognition of its etiology, it still presents a diagnostic problem. Diagnosis of toxoplasmosis is mainly based on the results of serological tests detecting anti-T. gondii-specific antibodies in the patient's serum sample. The specificities and sensitivities of serology tests depend mostly on the diagnostic antigen(s) used. Most of the commercial serological kits currently available are based on Toxoplasma lysate antigens (TLAs). In recent years, many studies showed that recombinant antigenic proteins of T. gondii may be an alternative source of antigens which are very useful for the serodiagnosis of toxoplasmosis. This article presents a review of current studies on the application and usefulness of different T. gondii recombinant antigens in serological tests for the diagnosis of human toxoplasmosis.

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“…The use of recombinant ROP antigens (rROP) has been largely described as the antigenic substrate to use in ELISA tests to detect infection with T. gondii (vanGelder et al, 1993;Aubert et al, 2000;Chang et al, 2011). rROP1 has shown its diagnostic value in IgG ELISA avidity tets for identification of acute infections (Holec-Gąsior et al, 2009, 2010a. There is data regarding the potential use of rROP2 for the diagnosis of acute toxoplasmosis that have focused on IgG reactivities (Martin et al, 1998;Chang et al, 2011).…”

Section: Rhoptries Antigens (Rop)mentioning

confidence: 99%

Toxoplasmosis: IgG Avidity and Its Implication in Serodiagnosis

Nissapatorn¹,

Sawangjareon²

2012

Trends in Immunolabelled and Related Techniques

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GRA2 and ROP1 Recombinant Antigens as Potential Markers for Detection of Toxoplasma gondii -Specific Immunoglobulin G in Humans with Acute Toxoplasmosis

Cited by 49 publications

References 25 publications

Toxoplasma gondii Antigens Recognized by IgG Antibodies Differ between Mice with and without Active Proliferation of Tachyzoites in the Brain during the Chronic Stage of Infection

Toxoplasma gondii Antigens Recognized by IgG Antibodies Differ between Mice with and without Active Proliferation of Tachyzoites in the Brain during the Chronic Stage of Infection

Toxoplasma gondii Recombinant Antigens as Tools for Serodiagnosis of Human Toxoplasmosis: Current Status of Studies

Toxoplasmosis: IgG Avidity and Its Implication in Serodiagnosis

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