The bioindicative assessment of the park ecosystems state in Kyiv (Ukraine) on the gradient of anthropogenic impact and urbanization by levels of life organization from the cell to the ecosystem was taken; the biotic diversity and state of the plant, bird, and wild bee communities were taken into account. The spatial analysis of the anthropogenic factors distribution was performed using GIS mapping. For the first time, ecosystem services of park ecosystems and their interrelationships with urbanization and biodiversity were studied for the Kyiv urban ecosystem. A system of indicators for rapid diagnostics of the degradation degree of urban parks at different levels of organization of life has been developed and tested. The monograph is intended for scientists, educators, conservationists,nature managers, and non-specialist audiences. It is recommended for making management decisions on the maintenance, conservation, and care of landscape areas in urban ecosystems.