Graduated embryo score (GES) is superior to day-3 morphologic criteria alone for predicting ART outcome

Graduated Embryo Score and soluble human leukocyte antigen-G expression improve assisted reproductive technology outcomes and suggest a basis for elective single-embryo transfer

“…Embryos were cultured individually in 20-L droplets of Early Cleavage medium (ECM; Sage) under oil at 37°C in a 5% CO 2 , 5% O 2 , 90% N 2 environment. All embryos were serially evaluated over a period of 72 hours after intracytoplasmic sperm injection and graded microscopically by GES (16). Embryos were transferred to a blastocyst medium 46 hours after intracytoplasmic sperm injection, because this may improve embryo development (Keskintepe et al, unpublished data).…”

“…We previously described a system for comprehensive morphologic evaluation of preimplantation embryos, the Graduated Embryo Score (GES) (15,16), which uses serial evaluations of early developmental milestones in individually cultured embryos, to generate a score out of 100 points on day 3 of culture. Since implementing the GES, we have achieved higher delivery rates from day 3 ET than we could from day 3 evaluation alone.…”

Gonadotropin-releasing hormone agonist/antagonist conversion with estrogen priming in low responders with prior in vitro fertilization failure

“…Embryos were cultured individually in 50-mL droplets of early cleavage medium (ECM) (Sage BioPharma, Pasadena, CA) under oil at 37 C in a 5% CO 2 , 5% O 2 , 90% N 2 environment. All embryos were serially evaluated over the 72 hours following ICSI, generating a graduated embryo score (GES) (17). Embryos were transferred to blastocyst medium (Irvine Scientific, Santa Ana, CA) 46 hours after ICSI (18).…”