Grafted-double walled carbon nanotubes as electrochemical platforms for immobilization of antibodies using a metallic-complex chelating polymer: Application to the determination of adiponectin cytokine in serum

Ojeda, Irene; Barrejón, Myriam; Arellano, Luis M.; González‐Cortés, Araceli; Yáñez‐Sedeño, Paloma; Langa, Fernando; Pingarrón, José M.

doi:10.1016/j.bios.2015.06.001

Cited by 53 publications

(28 citation statements)

References 28 publications

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“…The amount of Mix&Go used was that optimized previously in our group (Ojeda et al, 2015) and, in these studies, conventional HRP-Strep was used to label the detection antibody since the effect of the other variables should not depend on the type of label employed.…”

Section: Optimization Of the Experimental Variables Involved In The Pmentioning

confidence: 99%

“…This polymer uses ligands to bind Fc domains that mimic those binding domains from proteins A and G (Ooi et al, 2014), and can also interact strongly with electron donating moieties such as dissociated carboxyl groups. Therefore, combination of HOOC-MBs with the use of Mix&Go for antibody immobilization resulted in a convenient methodology to be applied as a general route for the preparation of electrochemical immunosensors (Ojeda et al, 2015). Capture antibodies immobilization was followed by a blocking step with ethanolamine (step 2) and the implementation of a sandwich-type immunoassay employing a biotinylated secondary antibody and poly-HRP-Strept for signal amplification (step 3).…”

Section: Analysis Of Spiked Urinementioning

confidence: 99%

“…These magnetic beads have demonstrated to be powerful tools for the preparation of electrochemical immunosensors enabling minimization of matrix effects in the analysis of complex samples (Zacco et al, 2006), (Ruiz-Valdepeñas Montiel et al, 2015). Covalent immobilization of the specific antibody for TGF-β1 (anti-TGF) was performed using Mix&Go, a polymer containing several metallic complexes selected for their efficiency to bind proteins (Ojeda et al, 2015). A sandwich-type immunoassay was designed using biotin-anti-TGF, and conjugation with peroxidase-labeled streptavidin (poly-HRP-Strept) polymer was used as to amplify the electrochemical detection.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine

Sánchez‐Tirado

Pérez‐Quintanilla

González‐Cortés

et al. 2017

Biosensors and Bioelectronics

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The first amperometric immunosensor for the quantification of TGF-β1, a cytokine proposed as a biomarker for patients having or at risk for renal disease, is described in this work. The immunosensor design involves disposable devices using carboxylic acid-functionalized magnetic microparticles supported onto screen-printed carbon electrodes and covalent immobilization of the specific antibody for TGF-β1 using Mix&Go polymer. A sandwich-type immunoassay was performed using biotin-anti-TGF and conjugation with peroxidase-labeled streptavidin (poly-HRP-Strept) polymer. Amperometric measurements were carried out at -0.20V by adding hydrogen peroxide solution onto the electrode surface in the presence of hydroquinone as the redox mediator. The calibration plot allowed a range of linearity extending between 15 and 3000pg/mL TGF-β1 which is adequate for the determination of the cytokine in plasma and urine. The limit of detection, 10pg/mL, is notably improved with respect to those obtained with ELISA kits. The usefulness of the immunosensor for the determination of low TGF-β1 concentrations in real samples was evaluated by analyzing spiked urine at different pg/mL concentration levels.

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Section: Optimization Of the Experimental Variables Involved In The Pmentioning

confidence: 99%

Section: Analysis Of Spiked Urinementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine

Sánchez‐Tirado

Pérez‐Quintanilla

González‐Cortés

et al. 2017

Biosensors and Bioelectronics

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“…Moreover, screen-printed electrodes (SPEs) are appropriate platforms for the immobilization of biomolecules, e.g. nucleic acid (Das et al 2014), enzymes (Henao-Escobar et al 2016) or antibodies (Ojeda et al 2015) onto the underlying working electrode surface in order to obtain a sensitive, selective, disposable electrochemical biosensor. SPE platforms are mostly based on carbon materials in which the nature, structural and physic-chemical properties of the carbonaceous materials have paid significant attention to the performance of the electrochemical (bio)sensors.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures

Hernández-Ibáñez

García‐Cruz

Montiel

et al. 2016

Biosensors and Bioelectronics

142

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Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures, Biosensors and Bioelectronic, http://dx.doi.org/10.1016/j.bios.2015.11.005 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. This novel electrochemical lactate biosensors analytical efficacy is explored towards the sensing of lactate in model (buffer) solutions and is found to exhibit a linear response towards lactate over the concentration range of 30.4 and 243.9 µM in phosphate buffer solution, with a corresponding limit of detection (based on 3-sigma) of 22.6 µM and exhibits a sensitivity of 3417 ± 131 µA M -1 according to the reproducibility study. These novel electrochemical lactate biosensors exhibit a high reproducibility, with a relative standard deviation of less than 3.8 % and an enzymatic response over 82 % after 5 months stored at 4 ºC. Furthermore, high performance liquid chromatography technique has been utilized to independently validate the electrochemical lactate biosensor for the determination of lactate in a commercial embryonic cell culture medium providing excellent agreement between the two analytical protocols.

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“…The histidine cluster His 433-X-His 435 was found to be surface accessible to affinity binding using these metals without the need for recombinant tags. Muir et al 124 prepared metal coordinating polymer substrates and screened a large range of transition metals to ) to achieve oriented immobilization of native anti-adiponectin antibody on carboxyphenyl multiwalled carbon nanotubes 125 and graphene oxide-carboxymethyl cellulose hybrid. 126 Recently, Welch et al 127 employed the chromium complex, [Cr(OH) 6 ] 3À , buffered with ethylenediamine as a wet chemical modification to ten commercial microtiter plates, postproduction, to improve antibody immobilization and ELISA performance.…”

Section: Metal Affinitymentioning

confidence: 99%

Orientation and characterization of immobilized antibodies for improved immunoassays (Review)

et al. 2017

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Orientation of surface immobilized capture proteins, such as antibodies, plays a critical role in the performance of immunoassays. The sensitivity of immunodiagnostic procedures is dependent on presentation of the antibody, with optimum performance requiring the antigen binding sites be directed toward the solution phase. This review describes the most recent methods for oriented antibody immobilization and the characterization techniques employed for investigation of the antibody state. The introduction describes the importance of oriented antibodies for maximizing biosensor capabilities. Methods for improving antibody binding are discussed, including surface modification and design (with sections on surface treatments, three-dimensional substrates, self-assembled monolayers, and molecular imprinting), covalent attachment (including targeting amine, carboxyl, thiol and carbohydrates, as well as “click” chemistries), and (bio)affinity techniques (with sections on material binding peptides, biotin-streptavidin interaction, DNA directed immobilization, Protein A and G, Fc binding peptides, aptamers, and metal affinity). Characterization techniques for investigating antibody orientation are discussed, including x-ray photoelectron spectroscopy, spectroscopic ellipsometry, dual polarization interferometry, neutron reflectometry, atomic force microscopy, and time-of-flight secondary-ion mass spectrometry. Future perspectives and recommendations are offered in conclusion.

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Grafted-double walled carbon nanotubes as electrochemical platforms for immobilization of antibodies using a metallic-complex chelating polymer: Application to the determination of adiponectin cytokine in serum

Cited by 53 publications

References 28 publications

Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine

Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine

Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures

Orientation and characterization of immobilized antibodies for improved immunoassays (Review)

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