Grafting of poly(<i>ε</i>-caprolactone) on electrospun gelatin nanofiber through surface-initiated ring-opening polymerization

Başaran, İhsan; Oral, Ayhan

doi:10.1080/00914037.2017.1417287

Cited by 8 publications

(7 citation statements)

References 38 publications

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“…Aqueous AcAc solutions can easily solubilize Gel due to its polar nature; in fact, Gel solutions in aqueous AcAc can be successfully electrospun into homogeneous Gel fibrillar scaffolds [18,21]. Lately, it has been shown by our group and other research groups that glacial AcAc solutions of PCL also present adequate properties for electrospinning, producing homogeneous fibrillar scaffolds [18,41,42].…”

Section: Introductionmentioning

confidence: 91%

“…It has adequate structural and mechanical stability for soft-tissue engineering applications; however, its hydrophobic character limits its ability to promote cell adhesion and proliferation [17,18]. On the other hand, gelatin (Gel) is a natural polymer derived from the partial hydrolysis of collagen, the major component of the ECM [19], that offers attractive biological advantages having the amino and carboxyl functional groups of collagen but displaying lower immunogenicity and antigenicity [20][21][22]. Nevertheless, Gel rapid dissolution and weak mechanical stability in physiological conditions restricts its use for tissue engineering applications [18,23,24].…”

Section: Introductionmentioning

confidence: 99%

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Single-step, acid-based fabrication of homogeneous gelatin-polycaprolactone fibrillar scaffolds intended for skin tissue engineering

et al. 2020

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Blends of natural and synthetic polymers have recently attracted great attention as scaffolds for tissue engineering applications due to their favorable biological and mechanical properties. Nevertheless, phase-separation of blend components is an important challenge facing the development of electrospun homogeneous fibrillar natural-synthetic polymers scaffolds; phase-separation can produce significant detrimental effects for scaffolds fabricated by electrospinning. In the present study, blends of gelatin (Gel; natural polymer) and polycaprolactone (PCL; synthetic polymer), containing 30 and 45 wt% Gel, were prepared using acetic acid as a ‘green’ sole solvent to straightforwardly produce appropriate single-step Gel-PCL solutions for electrospinning. Miscibility of Gel and PCL in the scaffolds was assessed and the morphology, chemical composition and structural and solid-state properties of the scaffolds were thoroughly investigated. Results showed that the two polymers proved miscible under the single-step solution process used and that the electrospun scaffolds presented suitable properties for potential skin tissue engineering applications. Viability, metabolic activity and protein expression of human fibroblasts cultured on the Gel-PCL scaffolds were evaluated using LIVE/DEAD (calcein/ethidium homodimer), MTT-Formazan and immunocytochemistry assays, respectively. In vitro results showed that the electrospun Gel-PCL scaffolds enhanced cell viability and proliferation in comparison to PCL scaffolds. Furthermore, scaffolds allowed fibroblasts expression of extracellular matrix proteins, tropoelastin and collagen Type I, in a similar way to positive controls. Results indicated the feasibility of the single-step solution process used herein to obtain homogeneous electrospun Gel-PCL scaffolds with Gel content ≥30 wt% and potential properties to be used as scaffolds for skin tissue engineering applications for wound healing.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Single-step, acid-based fabrication of homogeneous gelatin-polycaprolactone fibrillar scaffolds intended for skin tissue engineering

et al. 2020

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“…This hydrophilicity can be attributed to the multiple hydrophilic functional groups of Gel 58 . Other studies have reported higher hydrophilicity of the electrospun PCL/Gel scaffold (contact angles: ranged ~40-60°) in comparsion with PCL which is moderately hydrophobic (contact angle: ranged ~90-130°) [59][60][61][62] . At the study of a PCL/Gel based nanofibers wound dressing by Ajmal et al, the PCL contact angel was reported 100.1 ± 3.1° which decreased to 55.5 ± 2.1° after gelatin incorporation at PCL/Gel scaffold.…”

Section: Gc-ms Analysismentioning

confidence: 86%

A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold

Eskandarinia

Kefayat

Agheb

et al. 2020

Sci Rep

155

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one-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are composed of two layers with different properties have gained lots of attention. in the present study, polycaprolactone/gelatin (pcL/Gel) scaffold was electrospun on a dense membrane composed of polyurethane and ethanolic extract of propolis (pU/eep). the pU/eep membrane was used as the top layer to protect the wound area from external contamination and dehydration, while the PCL/Gel scaffold was used as the sublayer to facilitate cells' adhesion and proliferation. the bilayer wound dressing was investigated regarding its microstructure, mechanical properties, surface wettability, anti-bacterial activity, biodegradability, biocompatibility, and its efficacy in the animal wound model and histopathological analyzes. Scanning electron micrographs exhibited uniform morphology and bead-free structure of the PCL/Gel scaffold with average fibers' diameter of 237.3 ± 65.1 nm. Significant anti-bacterial activity was observed against Staphylococcal aureus (5.4 ± 0.3 mm), Escherichia coli (1.9 ± 0.4 mm) and Staphylococcus epidermidis (1.0 ± 0.2 mm) according to inhibition zone test. The bilayer wound dressing exhibited high hydrophilicity (51.1 ± 4.9°), biodegradability, and biocompatibility. The bilayer wound dressing could significantly accelerate the wound closure and collagen deposition in the Wistar rats' skin wound model. taking together, the pU/eep-pcL/Gel bilayer wound dressing can be a potential candidate for biomedical applications due to remarkable mechanical properties, biocompatibility, antibacterial features, and wound healing activities. Skin is always at the exposer of different types of damages 1. Severe skin damages can be life-threatening due to loss of human body fluids, electrolytes, and nutritional components from the wound area. Therefore, wound dressings have gained lots of attention 2. An ideal wound dressing should protect the wound from external contaminants and facilitate the healing process. However, one-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are

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“…Nonetheless, Gel dissolution and fast biodegradation in physiological conditions make it mechanically unstable and restrict its use in GTR membranes [10,16,17]. Blends of PCL and Gel can combine the intrinsic properties of both polymers resulting in materials with biologically favorable properties, specially blends with 30 w% Gel concentrations or higher [15,18,19], and modulated biodegradation rates, overcoming the respective disadvantages of PCL and Gel themselves [14,[18][19][20][21].…”

Section: Page 4 Of 38 Author Submitted Manuscript -Bmm-103219r1mentioning

confidence: 99%

Antibacterial composite membranes of polycaprolactone/gelatin loaded with zinc oxide nanoparticles for guided tissue regeneration

et al. 2020

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Grafting of poly(ε-caprolactone) on electrospun gelatin nanofiber through surface-initiated ring-opening polymerization

Cited by 8 publications

References 38 publications

Single-step, acid-based fabrication of homogeneous gelatin-polycaprolactone fibrillar scaffolds intended for skin tissue engineering

Single-step, acid-based fabrication of homogeneous gelatin-polycaprolactone fibrillar scaffolds intended for skin tissue engineering

A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold

Antibacterial composite membranes of polycaprolactone/gelatin loaded with zinc oxide nanoparticles for guided tissue regeneration

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