Grafting of silica with sulfobetaine polymers <i>via</i> aqueous reversible addition fragmentation chain transfer polymerization and its use as a stationary phase in HILIC

Wikberg, Erika; Verhage, Jeroen; Viklund, Camilla; Irgum, Knut

doi:10.1002/jssc.200800554

Cited by 38 publications

(16 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, a new phosphorylcholine‐type zwitterionic phase (Kromasil silica‐poly 2‐methacryloyloxyethyl phosphorylcholine, KS‐polyMPC) was synthesized by graft polymerization of MPC on the surface of porous Kromasil silica particles 54. Another new zwitterionic SP was synthesized and described in 55. Porous Purospher silica particles were first activated for vinylic polymerization by functionalization with 3‐methacryloyloxypropyl trimethoxysilane and subsequently dressed with zwitterionic grafts of the sulfoalkylbetaine type by polymerizing 3‐(2‐( N ‐methacryloyloxyethyl)‐ N , N ‐dimethylammonio)propane sulfonate.…”

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

HILIC methods in pharmaceutical analysis

Dejaegher

Heyden

2010

J of Separation Science

172

View full text Add to dashboard Cite

In this review, hydrophilic interaction liquid chromatographic (HILIC) applications for pharmaceutical analysis are discussed. The HILIC technique uses an aqueous/organic modifier mobile phase with a high organic modifier fraction and a hydrophilic stationary phase (SP). In general, the SPs are silica or polymer based. The silica-based columns are most often applied and are divided into bare-silica particle, chemically bonded silica particle, and monolithic phases. The bare-silica columns are most frequently used. The much less applied polymer-based columns are also divided into particle and monolithic phases. In this review, the applications are grouped and discussed according to the SP type used. Whenever possible, the considered HILIC application is compared with other HILIC methods, e.g. on other SPs, and/or with other separation modes, for that application. The advantages and drawbacks of HILIC are also discussed, as well as the method validation issues, when executed.

show abstract

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

HILIC methods in pharmaceutical analysis

Dejaegher

Heyden

2010

J of Separation Science

172

View full text Add to dashboard Cite

show abstract

“…In recent years, polymer-coating inorganic materials have brought great interests for serving as chromatographic packing materials [13,16,18,21,23,24]. In general, there are two different approaches, ''grafting to'' and ''grafting from'', for the modification of the surface of inorganic materials by organic polymer layers [25,26].…”

Section: Introductionmentioning

confidence: 99%

“…Some specially designed silica‐based stationary phases, such as Atlantis HILIC Silica and ZIC HILIC Silica, are commercially available for HILIC applications 2. In addition, several novel polar separation materials have been prepared and demonstrated good chromatographic performance for a variety of polar compounds under HILIC mode 13–18. However, the retention characteristics and separation selectivity of different types of separation materials are different under HILIC mode 19, 20.…”

Section: Introductionmentioning

confidence: 99%

Grafting of silica with a hydrophilic triol acrylamide polymer via surface‐initiated “grafting from” method for hydrophilic‐interaction chromatography

Peng

Yuan

2011

J of Separation Science

View full text Add to dashboard Cite

A novel hydrophilic polymer-coated silica sorbent has been prepared using the radical "grafting from" polymerization method through surface-bound azo initiators for hydrophilic-interaction chromatography (HILIC). The azo groups were introduced to the surface of silica gel through the reaction with amino groups on the surface of silica gel with 4,4'-azobis(4-cyanopentanoic acid chloride) (ACVC). The resultant azo-immobilized silica gel served as surface initiator to polymerize hydrophilic triol acrylamide monomer N-acryloyltris(hydroxymethyl) aminomethane (NA) in methanol to get hydrophilic polymer-coated silica sorbent. The obtained poly(NA)-coated silica (pNA-sil) was characterized by Fourier transform infrared spectroscopy (FT-IR), elemental analysis (EA), and nitrogen sorption porosimetry (NSP). Then the pNA-sil was packed into the stainless-steel column and evaluated in high-performance liquid chromatography (HPLC). Good chromatographic performance for the separation of peptides and nucleosides was obtained under HILIC mode. The results indicated that the pNA-sil stationary phase behaved as mixed-mode retention mechanisms of hydrophilic and ionic interactions. Furthermore, the pNA-sil phase was used to separate tryptic digest of β-casein and our results showed that more than 12 peptides peaks were resolved and well distributed within the elution window. Finally, the pNA-sil stationary phase was demonstrated to possess remarkable reproducibility and stability. Taken together, the pNA-sil stationary phase prepared in the current study offers a potential application in proteomics study.

show abstract

“…16 Compared with monolayer stationary phases, stationary phases functionalized with hydrophilic multilayer polymer have attracted more attention owing to their high hydrophilicity and large binding capacity. 17,18 As for hydrophilicity modifications of stationary phases with polymers, "grafting from" polymerization has been proved to be an efficient approach. It overmatches other polymerization methods concerning the generation of polymer chains with high grafting densities from initiators on the substrate.…”

Section: Introductionmentioning

confidence: 99%

Hyperbranched Polyglycerol Functionalized Silica Stationary Phase for Hydrophilic Interaction Liquid Chromatography

Zhang

et al. 2018

ANAL. SCI.

View full text Add to dashboard Cite

Surface-initiated anionic-ring-opening multibranching polymerization was employed to prepare a hyperbranched polyglycerol (HPG) functionalized silica stationary phase for hydrophilic interaction liquid chromatography (HILIC). The obtained stationary phase was characterized by Fourier-transform infrared spectrometry (FT-IR) and thermogravimetric analysis (TGA). The chromatographic properties of the prepared stationary phase were systematically investigated. The abundance and multitude distribution of hydroxyl groups in HPG endowed the stationary phase with improved hydrophilicity and enhanced separation performance compared with the stationary phase functionalized with monolayer of hydroxyl groups. The stationary phase showed excellent retention of various polar compounds, such as nucleosides, necleobases, phenols and sulfanilamides, indicating great potential in the separation of complex biosamples.

show abstract

Grafting of silica with sulfobetaine polymers via aqueous reversible addition fragmentation chain transfer polymerization and its use as a stationary phase in HILIC

Cited by 38 publications

References 33 publications

HILIC methods in pharmaceutical analysis

HILIC methods in pharmaceutical analysis

Grafting of silica with a hydrophilic triol acrylamide polymer via surface‐initiated “grafting from” method for hydrophilic‐interaction chromatography

Hyperbranched Polyglycerol Functionalized Silica Stationary Phase for Hydrophilic Interaction Liquid Chromatography

Contact Info

Product

Resources

About