Grafting PNIPAAm from β-barrel shaped transmembrane nanopores

Charan, Himanshu; Kinzel, Julia; Glebe, Ulrich; Anand, Deepak; Garakani, Tayebeh Mirzaei; Zhu, Leilei; Bocola, Marco; Schwaneberg, Ulrich; Böker, Alexander

doi:10.1016/j.biomaterials.2016.08.033

Cited by 27 publications

(32 citation statements)

References 80 publications

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“…PNIPAAm is particularly and extensively investigated due to its solubility in water and its LCST close to body temperature (around 36.5–37.5 °C) [18]. The hydrogel is easily accessible by radical polymerization to obtain variable architectures such as block copolymers [19,20,21,22,23], gels [24,25], or grafted polymers [26,27]. Since it was first reported in 1968 [28], several papers and reviews related to the employment of PNIPAAm in the field of biomedicine have been published.…”

Section: Introductionmentioning

confidence: 99%

Poly(N-isopropylacrylamide) and Copolymers: A Review on Recent Progresses in Biomedical Applications

Lanzalaco

Armelín

2017

Gels

330

233

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Abstract:The innate ability of poly(N-isopropylacrylamide) (PNIPAAm) thermo-responsive hydrogel to copolymerize and to graft synthetic polymers and biomolecules, in conjunction with the highly controlled methods of radical polymerization which are now available, have expedited the widespread number of papers published in the last decade-especially in the biomedical field. Therefore, PNIPAAm-based hydrogels are extensively investigated for applications on the controlled delivery of active molecules, in self-healing materials, tissue engineering, regenerative medicine, or in the smart encapsulation of cells. The most promising polymers for biodegradability enhancement of PNIPAAm hydrogels are probably poly(ethylene glycol) (PEG) and/or poly(ε-caprolactone) (PCL), whereas the biocompatibility is mostly achieved with biopolymers. Ultimately, advances in three-dimensional bioprinting technology would contribute to the design of new devices and medical tools with thermal stimuli response needs, fabricated with PNIPAAm hydrogels.

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Section: Introductionmentioning

confidence: 99%

Poly(N-isopropylacrylamide) and Copolymers: A Review on Recent Progresses in Biomedical Applications

Lanzalaco

Armelín

2017

Gels

330

233

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“…Expression of FhuA ΔCVF tev from T7 expression vector pPR-IBA1 was performed using the E. coli B E BL21 (DE3) omp8 strain as expression host according to previous descriptions [ 29 , 34 , 51 ]. FhuA ΔCVF tev was extracted from E. coli with SDS as solubilizing agent as described previously [ 19 , 29 , 34 ]. Refolding of FhuA ΔCVF tev in 1.25% SDS was performed by dialysis against 0.125 mM PE–PEG or 50 mM MPD, respectively [ 29 , 34 ].…”

Section: Methodsmentioning

confidence: 99%

“…FhuA ΔCVF tev was extracted from E. coli with SDS as solubilizing agent as described previously [ 19 , 29 , 34 ]. Refolding of FhuA ΔCVF tev in 1.25% SDS was performed by dialysis against 0.125 mM PE–PEG or 50 mM MPD, respectively [ 29 , 34 ]. Protein concentration was determined by bicinchoninic acid reaction (PierceTM BCA Protein Assay Kit, Thermo Fisher Scientific, Darmstadt, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Disadvantageous of detergents is the tremendous reduction of selectivity due to denaturing the protein or the reduction of productivity by detergent micelles since hydrophobic compounds are most likely located inside the hydrophobic micelle core. Recently, the small amphiphilic alcohol 2-methyl-2,4-pentanediol (MPD) was shown to successfully stabilize membrane proteins and enable characterization of protein modifications [ 33 – 34 ]. Polymerization of phenylacetylene in the presence of MPD molecules as refolding agent was carried out, reaching higher molecular weights and yields compared to catalysis with the micelle-forming refolding reagent PE–PEG.…”

Section: Introductionmentioning

confidence: 99%

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2-Methyl-2,4-pentanediol (MPD) boosts as detergent-substitute the performance of ß-barrel hybrid catalyst for phenylacetylene polymerization

Kinzel¹,

Sauer²,

Bocola³

et al. 2017

Beilstein J. Org. Chem.

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Covering hydrophobic regions with stabilization agents to solubilize purified transmembrane proteins is crucial for their application in aqueous media. The small molecule 2-methyl-2,4-pentanediol (MPD) was used to stabilize the transmembrane protein Ferric hydroxamate uptake protein component A (FhuA) utilized as host for the construction of a rhodium-based biohybrid catalyst. Unlike commonly used detergents such as sodium dodecyl sulfate or polyethylene polyethyleneglycol, MPD does not form micelles in solution. Molecular dynamics simulations revealed the effect and position of stabilizing MPD molecules. The advantage of the amphiphilic MPD over micelle-forming detergents is demonstrated in the polymerization of phenylacetylene, showing a ten-fold increase in yield and increased molecular weights.

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“…ECM prevented the progression of maladaptive remodeling after cardiac infarct and ultimately decreased left ventricular compliance, promoted angiogenesis, and prevented left ventricular dilatation, scarring, and thinning. 47 ECM has also been used to coat synthetic polymer scaffolds. Solubilized small intestine submucosa (SIS) ECM powder was used to coat polylactic-co-glycolic acid (PLGA) for use in nucleus pulposus regeneration of intervertebral discs.…”

Section: Ecm Ornamentationmentioning

confidence: 99%

Utility of extracellular matrix powders in tissue engineering

et al. 2018

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Extracellular matrix (ECM) materials have had remarkable success as scaffolds in tissue engineering (TE) and as therapies for tissue injury whereby the ECM microenvironment promotes constructive remodeling and tissue regeneration. ECM powder and solubilized derivatives thereof have novel applications in TE and RM afforded by the capacity of these constructs to be dynamically modulated. The powder form allows for effective incorporation and penetration of reagents; hence, ECM powder is an efficacious platform for 3D cell culture and vehicle for small molecule delivery. ECM powder offers minimally invasive therapy for tissue injury and successfully treatment for wounds refractory to first-line therapies. Comminution of ECM and fabrication of powder-derived constructs, however, may compromise the biological integrity of the ECM. The current lack of optimized fabrication protocols prevents a more extensive and effective clinical application of ECM powders. Further study on methods of ECM powder fabrication and modification is needed.

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Grafting PNIPAAm from β-barrel shaped transmembrane nanopores

Cited by 27 publications

References 80 publications

Poly(N-isopropylacrylamide) and Copolymers: A Review on Recent Progresses in Biomedical Applications

Poly(N-isopropylacrylamide) and Copolymers: A Review on Recent Progresses in Biomedical Applications

2-Methyl-2,4-pentanediol (MPD) boosts as detergent-substitute the performance of ß-barrel hybrid catalyst for phenylacetylene polymerization

Utility of extracellular matrix powders in tissue engineering

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