Grafting versus Crosslinking of Silk Fibroin-g-PNIPAM via Tyrosine-NIPAM Bridges

Radu, I.; Bîru, Elena Iuliana; Damian, Celina Maria; Ion, Andreea-Cristina; Iovu, Horia; Tanasă, Eugenia; Zaharia, Cătălin; Gălățeanu, Bianca

doi:10.3390/molecules24224096

Cited by 26 publications

(16 citation statements)

References 52 publications

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“…This proved that the polymerization of NIPAm was conducted successfully, for both synthesis approaches. Similar findings were documented by Elashnikov et al [ 16 ] and Radu et al [ 17 ]. Furthermore, broadening of the characteristic peak at 3300 cm −1 resulted from an overlap with O-H stretching signatures, due to the interaction between polymer chains and neighboring water molecules [ 18 ].…”

Section: Resultssupporting

confidence: 91%

Development of Thermally Responsive PolyNIPAm Microcarrier for Application of Cell Culturing—Part I: A Feasibility Study

et al. 2021

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Poly(N-isopropylacrylamide) (polyNIPAm) microspheres were synthesized via the suspension polymerization technique. Thermal and redox initiators were compared for the polymerization, in order to study the effect of initiator type on the surface charge and particle size of polyNIPAm microspheres. The successful polymerization of NIPAm was confirmed by FTIR analysis. Microspheres of diameter >50 µm were synthesized when a pair of ammonium persulfate (APS) and N,N,N’,N’-tetramethylene-diamine (TEMED) redox initiators was used, whilst relatively small microspheres of ~1 µm diameter were produced using an Azobis-isobutyronitrile (AIBN) thermal initiator. Hence, suspension polymerization using a redox initiator pair was found to be more appropriate for the synthesis of polyNIPAm microspheres of a size suitable for human embryonic kidney (HEK) cell culturing. However, the zeta potential of polyNIPAm microspheres prepared using an APS/TEMED redox initiator was significantly more negative than AIBN thermal initiator prepared microspheres and acted to inhibit cell attachment. Conversely, strong cell attachment was observed in the case of polyNIPAm microspheres of diameter ~90 µm, prepared using an APS/TEMED redox initiator in the presence of a cetyl trimethyl ammonium bromide (CTAB) cationic surfactant; demonstrating that surface charge modified polyNIPAm microspheres have great potential for use in cell culturing.

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Section: Resultssupporting

confidence: 91%

Development of Thermally Responsive PolyNIPAm Microcarrier for Application of Cell Culturing—Part I: A Feasibility Study

et al. 2021

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“…Bombyx mori proteins have been intensively studied for their biocompatibility, great mechanical properties, tunable biodegradation process, easy processing, and favorable source supply. Silk is composed of two major proteins: silk fibroin (fibrous protein) and silk sericin (globular protein) [ 4 , 5 , 6 , 7 , 8 ]. Silk fibroin is the main protein with large usage in the biomedical field.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Interaction of Doxorubicin-Loaded Silk Sericin Nanocarriers with MCF-7 Breast Cancer Cells Leads to DNA Damage

et al. 2021

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In this paper, Bombyx mori silk sericin nanocarriers with a very low size range were obtained by nanoprecipitation. Sericin nanoparticles were loaded with doxorubicin, and they were considered a promising tool for breast cancer therapy. The chemistry, structure, morphology, and size distribution of nanocarriers were investigated by Fourier transformed infrared spectroscopy (FTIR–ATR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and dynamic light scattering (DLS). Morphological investigation and DLS showed the formation of sericin nanoparticles in the 25–40 nm range. FTIR chemical characterization showed specific interactions of protein–doxorubicin–enzymes with a high influence on the drug delivery process and release behavior. The biological investigation via breast cancer cell line revealed a high activity of nanocarriers in cancer cells by inducing significant DNA damage.

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“…Bombyx mori cocoons were kindly provided by SC Sericarom SA, sodium carbonate (Na 2 CO 3 ) and sodium bicarbonate (NaHCO 3 ) by Aldrich, sodium dodecyl sulfate was obtained from Merck and lithium bromide (LiBr) was purchased from Honeywell. Silk fibroin was obtained from silkworm cocoons by the degumming method [ 26 ]. Briefly, Chopped Bombyx mori cocoons were degummed by washing three times in hot aqueous solution of 0.5% ( w / v ) Na 2 CO 3 , NaHCO 3 and sodium dodecyl sulfate, and then rinsed thoroughly with distilled water to extract the sericin protein and other components.…”

Section: Methodsmentioning

confidence: 99%

Silk ProteinsEnriched Nanocomposite Hydrogels Based on Modified MMT Clay and Poly(2-hydroxyethyl methacrylate-co-2-acrylamido-2-methylpropane Sulfonic Acid) Display Favorable Properties for Soft Tissue Engineering

et al. 2022

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Due to their remarkable structures and properties, three-dimensional hydrogels and nanostructured clay particles have been extensively studied and have shown a high potential for tissue engineering as solutions for tissue defects. In this study, four types of 2-hydroxyethyl methacrylate/2-acrylamido-2-methylpropane sulfonic acid/montmorillonite (HEMA/AMPSA/MMT) hydrogels enriched with sericin, and fibroin were prepared and studied in the context of regenerative medicine for soft tissue regenerative medicine. Our aim was to obtain crosslinked hydrogel structures using modified montmorillonite clay as a crosslinking agent. In order to improve the in vitro and in vivo biocompatibility, silk proteins were further incorporated within the hydrogel matrix. Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR) were performed to prove the chemical structures of the modified MMT and nanocomposite hydrogels. Swelling and rheological measurements showed the good elastic behavior of the hydrogels due to this unique network structure in which modified MMT acts as a crosslinking agent. Hydrogel biocompatibility was assessed by MTT, LDH and LIVE/DEAD assays. The hydrogels were evaluated for their potential to support adipogenesis in vitro and human stem cells isolated from adipose tissue were seeded in them and induced to differentiate. The progress was assessed by evaluation of expression of adipogenic markers (ppar-γ2, perilipin) evaluated by qPCR. The potential of the materials to support tissue regeneration was further evaluated on animal models in vivo. All materials proved to be biocompatible, with better results on the 95% HEMA 5% AMPSA enriched with sericin and fibroin material. This composition promoted a better development of adipogenesis compared to the other compositions studied, due the addition of sericin and fibroin. The results were confirmed in vivo as well, with a better progress of soft tissue regeneration after implantation in mice. Therefore, hydrogel 95% HEMA 5% AMPSA enriched with sericin as well as fibroin showed the best results that recommend it for future soft tissue engineering application.

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Grafting versus Crosslinking of Silk Fibroin-g-PNIPAM via Tyrosine-NIPAM Bridges

Cited by 26 publications

References 52 publications

Development of Thermally Responsive PolyNIPAm Microcarrier for Application of Cell Culturing—Part I: A Feasibility Study

Development of Thermally Responsive PolyNIPAm Microcarrier for Application of Cell Culturing—Part I: A Feasibility Study

In Vitro Interaction of Doxorubicin-Loaded Silk Sericin Nanocarriers with MCF-7 Breast Cancer Cells Leads to DNA Damage

Silk ProteinsEnriched Nanocomposite Hydrogels Based on Modified MMT Clay and Poly(2-hydroxyethyl methacrylate-co-2-acrylamido-2-methylpropane Sulfonic Acid) Display Favorable Properties for Soft Tissue Engineering

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