2012
DOI: 10.1021/pr201274r
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Gram-negative and Gram-Positive Bacterial Infections Give Rise to a Different Metabolic Response in a Mouse Model

Abstract: Metabolomics has become an important tool to study host-pathogen interactions and to discover potential novel therapeutic targets. In an attempt to develop a better understanding of the process of pathogenesis and the associated host response we have used a quantitative 1H NMR approach to study the metabolic response to different bacterial infections. Here we describe that metabolic changes found in serum of mice that were infected with Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli and Pseu… Show more

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Cited by 62 publications
(53 citation statements)
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“…Metabolomics is becoming increasingly important for the analysis of host-pathogen interactions, particularly in the discovery of biomarkers of disease (11). Recent studies have shown that metabolomics can be used to identify metabolites whose levels are altered in response to an infection (10,(49)(50)(51).…”
Section: Discussionmentioning
confidence: 99%
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“…Metabolomics is becoming increasingly important for the analysis of host-pathogen interactions, particularly in the discovery of biomarkers of disease (11). Recent studies have shown that metabolomics can be used to identify metabolites whose levels are altered in response to an infection (10,(49)(50)(51).…”
Section: Discussionmentioning
confidence: 99%
“…Recent studies have shown that metabolomics can be used to identify metabolites whose levels are altered in response to an infection (10,(49)(50)(51). In particular, with respect to bacterial infections, the rapid emergence of multidrug-resistant Gram-positive and Gram-negative bacteria fuels the need for novel strategies in diagnosis and treatment (11). Diagnosis primarily relies on nonmolecular tests that are time-and labor-intensive.…”
Section: Discussionmentioning
confidence: 99%
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“…Metabolomics quantifies an organism's metabolite repertoire in a given environment [91], that is, by NMR spectroscopy and chromatography-coupled (tandem) mass spectrometry [92]. Quantitative 1 H NMR analysis was applied to detect unique metabolic responses in the serum of infected mice to gram-positive (Staphylococcus aureus, Streptococcus pneumoniae) and gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria [91].…”
Section: Metabolomicsmentioning
confidence: 99%
“…A serial dilution down to 10 À3 was performed, and 100 ll of each dilution was plated in duplicate onto BHI agar containing 200 lg ml À1 kanamycin (Sigma-Aldrich) with subsequent overnight incubation at 37°C. (The insertion of kanamycin resistance genes into the bacterial chromosome guarantees the selective growth of the bioluminescent S. aureus Xen29 strain on media containing kanamycin 200 lg ml À1 , (Hoerr et al 2012). This process was then repeated on the second square called 'Count II' for the same day ( Fig.…”
Section: Persistence Of Staphylococcus Aureus On Euro Currencymentioning
confidence: 99%