Gram-positive cell wall structure of the A3Î³ type in heliobacteria

Pickett, Mark W.; Weiss, Norbert; Kelly, David J.

doi:10.1111/j.1574-6968.1994.tb07135.x

Cited by 11 publications

(7 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The large percentage of the fatty acids in the plasma membrane contain branched chains, a feature characteristic of gram-positive bacteria (Beck et al 1990). Using partially hydrolyzed cell wall preparations, the cell wall structure was determined to be A3c type with L,L-diaminopimelate at position 3, D-alanine in position 4, and a glycine interpeptide bridge (Pickett et al 1994a). The A3c type cell wall structure is found in other gram-positive bacteria such as Clostridium perfringens and Nocardioides simplex (Pickett et al 1994a).…”

Section: Discovery and Classification Of Heliobacteriamentioning

confidence: 99%

“…Using partially hydrolyzed cell wall preparations, the cell wall structure was determined to be A3c type with L,L-diaminopimelate at position 3, D-alanine in position 4, and a glycine interpeptide bridge (Pickett et al 1994a). The A3c type cell wall structure is found in other gram-positive bacteria such as Clostridium perfringens and Nocardioides simplex (Pickett et al 1994a). Similar to Clostridia, heliobacteria also do not contain pyruvate dehydrogenase but rather contain pyruvate:ferredoxin oxidoreductase (Pickett et al 1994b).…”

Section: Discovery and Classification Of Heliobacteriamentioning

confidence: 99%

See 1 more Smart Citation

Heliobacterial photosynthesis

Heinnickel

Golbeck

2007

Photosynth Res

View full text Add to dashboard Cite

Heliobacteria contain Type I reaction centers (RCs) and a homodimeric core, but unlike green sulfur bacteria, they do not contain an extended antenna system. Given their simplicity, the heliobacterial RC (HbRC) should be ideal for the study of a prototypical homodimeric RC. However, there exist enormous gaps in our knowledge, particularly with regard to the nature of the secondary and tertiary electron acceptors. To paraphrase S. Neerken and J. Amesz (2001 Biochim Biophys Acta 1507:278-290): with the sole exception of primary charge separation, little progress has been made in recent years on the HbRC, either with respect to the polypeptide composition, or the nature of the electron acceptor chain, or the kinetics of forward and backward electron transfer. This situation, however, has changed. First, the low molecular mass polypeptide that contains the terminal FA and FB iron-sulfur clusters has been identified. The change in the lifetime of the flash-induced kinetics from 75 ms to 15 ms on its removal shows that the former arises from the P798+ [FA/FB]- recombination, and the latter from P798+ FX- recombination. Second, FX has been identified in HbRC cores by EPR and Mössbauer spectroscopy, and shown to be a [4Fe-4S]1+,2+ cluster with a ground spin state of S=3/2. Since all of the iron in HbRC cores is in the FX cluster, a ratio of approximately 22 Bchl g/P798 could be calculated from chemical assays of non-heme iron and Bchl g. Third, the N-terminal amino acid sequence of the FA/FB-containing polypeptide led to the identification and cloning of its gene. The expressed protein can be rebound to isolated HbRC cores, thereby regaining both the 75 ms kinetic phase resulting from P798+ [FA/FB]- recombination and the light-induced EPR resonances of FA- and FB-. The gene was named 'pshB' and the protein 'PshB' in keeping with the accepted nomenclature for Type I RCs. This article reviews the current state of knowledge on the structure and function of the HbRC.

show abstract

Section: Discovery and Classification Of Heliobacteriamentioning

confidence: 99%

Section: Discovery and Classification Of Heliobacteriamentioning

confidence: 99%

Heliobacterial photosynthesis

Heinnickel

Golbeck

2007

Photosynth Res

View full text Add to dashboard Cite

show abstract

“…Heliobacteria are anoxygenic phototrophic bacteria that contain bacteriochlorophyll g [1–3]. Heliobacteria differ from classical anoxyphototrophs – the purple and green bacteria – in many respects, including pigments [4–6], fine structure [7], cell wall structure [8], and ecology [9].…”

Section: Introductionmentioning

confidence: 99%

Molecular evidence that the capacity for endosporulation is universal among phototrophic heliobacteria

Kimble-Long

Madigan

2001

FEMS Microbiology Letters

View full text Add to dashboard Cite

Although enrichment cultures for anoxygenic phototrophic heliobacteria commonly contain sporulating cells, once strains of heliobacteria are obtained in pure culture, they all but cease to sporulate. In fact, some species of heliobacteria have never been observed to sporulate. Thus, despite their phylogenetic connection to endospore-forming bacteria, the question of sporulation capacity in heliobacteria remains open. We have investigated this problem using PCR and Southern hybridization as tools and show here that all recognized species of heliobacteria tested, as well as several unclassified strains, contain homologs to the ssp genes of Clostridium and Bacillus species, genes that encode key sporulation-specific proteins. It can therefore be concluded that as a group, heliobacteria are likely all to be endospore-forming bacteria in agreement with their phylogenetic placement within the 'low GC' Gram-positive bacteria.

show abstract

“…Heliobacteria are anoxygenic phototrophic bacteria that contain bacteriochlorophyll g [1^3]. Heliobacteria di¡er from classical anoxyphototrophs^the purple and green bacteria^in many respects, including pigments [4^6], ¢ne structure [7], cell wall structure [8], and ecology [9].…”

Section: Introductionmentioning

confidence: 99%

Molecular evidence that the capacity for endosporulation is universal among phototrophic heliobacteria

Kimble-Long

2001

FEMS Microbiology Letters

View full text Add to dashboard Cite

Although enrichment cultures for anoxygenic phototrophic heliobacteria commonly contain sporulating cells, once strains of heliobacteria are obtained in pure culture, they all but cease to sporulate. In fact, some species of heliobacteria have never been observed to sporulate. Thus, despite their phylogenetic connection to endospore-forming bacteria, the question of sporulation capacity in heliobacteria remains open. We have investigated this problem using PCR and Southern hybridization as tools and show here that all recognized species of heliobacteria tested, as well as several unclassified strains, contain homologs to the ssp genes of Clostridium and Bacillus species, genes that encode key sporulation-specific proteins. It can therefore be concluded that as a group, heliobacteria are likely all to be endosporeforming bacteria in agreement with their phylogenetic placement within the`low GC' Gram-positive bacteria. ß

show abstract

Gram-positive cell wall structure of the A3Î³ type in heliobacteria

Cited by 11 publications

References 20 publications

Heliobacterial photosynthesis

Heliobacterial photosynthesis

Molecular evidence that the capacity for endosporulation is universal among phototrophic heliobacteria

Molecular evidence that the capacity for endosporulation is universal among phototrophic heliobacteria

Contact Info

Product

Resources

About