Granular Acute Lymphoblastic Leukemia

Stein, Preston; Peiper, Stephen C.; Butler, Debra L.; Melvin, Susan L.; Williams, Dorothy L.; Stass, Sanford A.

doi:10.1093/ajcp/79.4.426

Cited by 42 publications

(17 citation statements)

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“…The only My9 + case had unusual features: the cells were My7 + and showed granular electrolucent inclusions at EM, as previously described in some common-ALL (Stein et al, 1983) but showed otherwise features of B-lineage commitment (B4+, Leul2+ and Ig gene rearrangement).…”

Section: Comparison With Okml In Myeloid Leukemiassupporting

confidence: 63%

“…This study also demonstrates the specificity of My9 for AML as all but one of the ALL tested were My9 -. The only My9 + case had unusual features: the cells were My7 + and showed granular electrolucent inclusions at EM, as previously described in some common-ALL (Stein et al, 1983) but showed otherwise features of B-lineage commitment (B4+, Leul2+ and Ig gene rearrangement). Our findings with the McAb 3C5, expressed almost exclusively in myeloblasts (M1 and few M2 cases), and in B-lineage lyrnphoblasts, suggest that the antigen detected by this reagent is restricted to an early precursor cell, possibly common to lymphoblasts and myeloblasts.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of myeloid leukemias with monoclonal antibodies 3C5 and MY9

Matutes

Rodriguez

Polli

et al. 1985

Hematological Oncology

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The expression of two membrane antigens identified by the monoclonal antibodies (McAb) My9 and 3C5 has been investigated in cells from 80 acute leukemias. My9 was positive in the blasts of 33 out of the 38 (87 per cent) cases of acute myeloid leukemia (AML) tested, regardless of FAB subtype, and in 13 of 18 (72 per cent) cases of chronic granulocytic leukemia (CGL) in myeloid blast crisis. The reactivity of 3C5 was confined to myeloblastic (M1) AML, 85 per cent of cases, and to lymphoblastic leukemia (ALL) of B-lineage, 70 per cent of cases, including CGL in lymphoid transformation. My9 was negative in ALL except for an unusual case. The phenotype My9+, 3C5+ was seen exclusively in M1 (69 per cent) and M2 (14 per cent) AML. Ultrastructural analysis with the immunogold method in combination with the myeloperoxidase (MPO) reaction showed that expression of My9 increased in parallel with MPO activity whereas 3C5 was expressed mainly in myeloblasts with little MPO content. We conclude that the use of these two McAb will contribute to the diagnosis and classification of AML and may throw some light to the pathogenesis of biphenotypic acute leukemias, including TdT + AML.

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Section: Comparison With Okml In Myeloid Leukemiassupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Characterization of myeloid leukemias with monoclonal antibodies 3C5 and MY9

Matutes

Rodriguez

Polli

et al. 1985

Hematological Oncology

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“…It has been reported mainly with male gender, precursor B -ALL subtype same like our patient. The incidence according to literature is 2-7% and mainly during childhood [2,3] and looks like it is connected with Down syndrome [1,4]. One of the features is azurophilic cytoplasmic granules, and it is one of the defining criteria of Granular ALL.…”

Section: Discussionmentioning

confidence: 99%

Granular lymphoblast in a case of acute lymphoblastic leukemia: A rare morphology

Mohamed¹,

Soliman²,

Yassin³

2016

IJCRI

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International Journal of Case Reports and Images (IJCRI) is an international, peer reviewed, monthly, open access, online journal, publishing high-quality, articles in all areas of basic medical sciences and clinical specialties.Aim of IJCRI is to encourage the publication of new information by providing a platform for reporting of unique, unusual and rare cases which enhance understanding of disease process, its diagnosis, management and clinico-pathologic correlations. IJCRI publishes Review

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“…Since then, several cases of granular ALL were reported in both adults and children. [6][7][8][9][10][11][12][13][14] Most of these cases had CALLA phenotype, but the blasts were of both L1 and L2 FAB subtypes and were positive for PAS and negative for SBB and MPOX. The granules or intracytoplasmic inclusions described were variable in size, color and shape.…”

Section: Discussionmentioning

confidence: 99%

Granular Acute Lymphoblastic Leukemia: Report of Two Cases and Review of the Literature

et al. 1996

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The classification of acute leukemias into lymphoid leukemia (ALL) and nonlymphoid leukemia (ANLL) is of critical therapeutic importance. The differentiation of ALL from ANLL is usually achieved through morphologic, cytochemical and immunophenotypic analysis of leukemic blasts. One of the main morphologic criteria in the differentiation between the two types is the presence of granules in the cytoplasm of ANLL blasts. There have been scattered reports of patients with ALL who have lymphoblasts with azurophilic cytoplasmic granules. We describe two cases of granular ALL with review of the literature. Case Report Case 1A 22-year-old Saudi male was referred to King Khalid University Hospital (KKUH) in April 1995 with a diagnosis of ALL. He presented with malaise and shortness of breath for one month prior to admission. On examination, he was pale with no jaundice or cyanosis. He had generalized lymphadenopathy and hepatosplenomegaly. Chest and central nervous system (CNS) examinations were unremarkable. The diagnosis of ALL was confirmed (see below) and chemotherapy was started using hydroxyurea 2 g, p.o., q.i.d., for three days, followed by mitoxantrone 12 mg/m 2 intravenously (IV) daily for three days, vincristine 1.4 mg/m 2 IV weekly for seven weeks, and methylprednisolone 60 mg/m 2 IV daily for 21 days. Because of CNS involvement, he was started on intrathecal (IT) methotrexate (MTX) 10 mg, cytosine arabinoside (Ara-C) 50 mg and hydrocortisone 50 mg three times a week for a total of 10 doses. Induction was complicated by fever and neutropenia and he was managed with broad spectrum antibiotics and amphotericin-B. He did not achieve complete remission. Reinduction was started, but the patient died of neutropenia and septic shock. Hematological EvaluationOn admission, complete blood count (CBC) revealed a white blood count (WBC) of 23.8xl0 9 /L, hemoglobin (Hb) of 107 g/L, and platelet count of 59xl0 9 /L. The WBC differential count showed 8% neutrophils, 70% lymphocytes and 20% blasts. Bone marrow (BM) aspirate revealed hypercellular marrow with 55% blasts. The blasts were variable in size with some showing high nuclear cytoplasmic (N/C) ratio, while others were larger with low N/C ratio and prominent nucleoli. The morphology was that of L2 by the French American British (FAB) classification of ALL.1 Fifteen percent of the blasts contained cytoplasmic granules that were variable in size and stained purple by May-Giemsa stain. The number of granules was also variable, but most of the blasts contained more than three granules. Occasional hand mirror cells were also present. Auer rods were absent.The blasts were negative for Sudan black (SBB) and chloroacetate esterase (CAE) and positive for periodic acid Schiff (PAS), nonspecific esterase (NSE) without inhibition by sodium fluoride (NaFl), and weakly positive for acid phosphatase (AP). Flow cytometry was performed on the bone marrow aspirate and showed the blasts to be positive for CD10 (CALLA), CD19, CD20, CD22 and HLADR and negative for CD7, CD3, CD13 and CD33. T...

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Granular Acute Lymphoblastic Leukemia

Cited by 42 publications

References 0 publications

Characterization of myeloid leukemias with monoclonal antibodies 3C5 and MY9

Characterization of myeloid leukemias with monoclonal antibodies 3C5 and MY9

Granular lymphoblast in a case of acute lymphoblastic leukemia: A rare morphology

Granular Acute Lymphoblastic Leukemia: Report of Two Cases and Review of the Literature

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