Granular Formulation Test of Pseudomonas fluorescens P60 for Controling Bacterial Wilt (Ralstonia solanacearum) of Tomato In Planta

Abstract

Ralstonia solanacearum is the most devastating bacteria. Pseudomonas fluorescens P60 is a bacterial antagonist. This research aimed to study shelf life, antagonism and the effect of granular application of P. fluorescens P60 to control bacterial wilt and growth of tomato in planta. The research was conducted at the Plant Protection Laboratory and the screen house, Faculty of Agriculture, Jenderal Soedirman University, from October 2018 to March 2019. A randomized block design was used with six treatments and five replicates. The treatments were control, R. solanacearum + 1, 5, 10, and 15 g the granule, and bactericide (Agrimycine sulfate 20%). Variables observed were population density, clear zone, incubation period, disease incidence, disease intensity, area under disease progress curve (AUDPC), crop height, root length, crops fresh weights, and phenolic compound content qualitatively. Result showed that the formulation up to 10 weeks still performed a high P. fluorescens P60 population and good activity. All the granular and the bactericide effectively suppressed the disease indicated by the lenghtening incubation period of 22.77-26.25%, reducing the disease incidence as 60-85%, decreasing disease intensity as 65-85%, and decreasing AUDPC as 75.69-86.11%-days, increasing phenolic compound content qualitatively, and increasing crop height between 24.85-36.17%, and fresh weight between 46.04-57.13%.

Secondary Metabolites Application of Two Pseudomonas fluorescens isolates and Two Trichoderma Harzianum Isolates in Combination Against Postharvest Anthracnose in Papaya

“…Propagation of P. fluorescens was carried out with golden snail broth and placed in sterile jerry cans (Soesanto et al, 2019). Antagonist suspension of P. fluorescens was shaken (Daiki Orbital Shaker) for 3 days at 150 rpm at room temperature (Soesanto et al, 2010).…”

“…Biological control is an alternative control method to reduce the use of synthetic pesticides (Thambugala et al, 2020). Pseudomonas fluorescens is a biological control bacteria widely used to control plant diseases (Soesanto et al, 2019), including postharvest diseases (Talibi et al, 2014;Sudha et al, 2021). In addition, the antagonist fungus Trichoderma harzianum has also been widely used (Soesanto et al, 2020;Zin & Badaluddin, 2020).…”

“…Antagonistic bacteria P. fluorescens can produce secondary metabolites that play a role in killing directly or only inhibiting pathogens (Soesanto et al, 2019). T. harzianum is capable of producing secondary metabolites that can inhibit the growth of other fungi or even kill them (Braun et al, 2018;Soesanto et al, 2020).…”

Application of Secondary Metabolites of Two Pseudomonas fluorescens Isolates to Control Bacterial Wilt of Potato

“…The secondary metabolites of antagonistic bacteria were prepared using gold snail broth. The golden snail meat is washed with clean water, after being washed it is weighed as much as 400 g for 1 liter of water, boiled until cooked and added 2 g of shrimp paste per 1 liter of water [13]. Once cooked, the broth is filtered through sterile cotton and placed in a sterile drum under heating, sealed and cooled.…”

Potency of secondary metabolites of Trichoderma asperellum and Pseudomonas fluorescens in the growth of cocoa plants affected by vascular streak dieback

“…This condition increases the phenol content in plants. Agrios (2005) stated that the increase in phenols in the plants because the secondary metabolites of antagonistic fungi are absorbed by plants and translocated systemically to all parts of the plant, giving rise to substances that are responsible for affected resistance, including phenol compounds In addition to antagonistic fungi, Soesanto and Termoshuizen (2001), and Jankiewicz and Koltonowicz (2012) stated that P. fluorescens colonize roots to stimulate plants, increasing the production of secondary metabolite compounds so that they play a role in plant resistance. Hanada et al (2010) reported that Trichoderma spp.…”