Granulation and immobilisation of methanogenic and sulfate-reducing bacteria in high-rate anaerobic reactors

Visser, André W.; Alphenaar, P.A.; Gao, Yaohuan; Rossum, G. van; Lettinga, G.

doi:10.1007/bf00175750

Cited by 51 publications

(30 citation statements)

References 22 publications

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“…Pure and mixed cultures of sulfatereducing bacteria cultivated in the laboratory often aggregate or stick to surfaces (Nielsen, 1987). Also they can form biofilms on inert carrier material under turbulent flow conditions (Hulshoff et al, 2004;van Houten el al., 1994;Visser et al, 1993). However, sulfate reducers ability to form biofilms on elemental sulfur in turbulent anaerobic reactors has not been described before.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen sulfide production from elemental sulfur by Desulfovibrio desulfuricans in an anaerobic bioreactor

Escobar

Bravo

Hernández

et al. 2007

Biotech & Bioengineering

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Feasibility of elemental sulfur reduction by Desulfovibrio desulfuricans in anaerobic conditions in a stirred reactor was studied. Hydrogen was used as energy source, whereas the carbonated species were bicarbonate and yeast extract. Attention was paid to reactor engineering aspects, biofilm formation on the sulfur surface, hydrogen sulfide formation rate and kinetics limitations of the sulfur reduction. D. desulfuricans formed stable biofilms on the sulfur surface. It was found that active sulfur surface availability limits the reaction rate. The reaction rate was first order with respect to sulfur and hydrogen velocity had no effect in the reaction rate for the range 8.2 x 10(-2) to 4.1 x 10(-1) Nm(3) m(-2) min(-1). At a superficial gas velocity (u(G)) = 3.1 x 10(-2) Nm(3) m(-2) min(-1), H(2)S(g) production rate decreased due to a deficient H(2)S stripping. A maximum H(2)S(g) production rate of 2.1 g H(2)S L(-1) d(-1) was achieved during 5 days with an initial sulfur density of 4.7% (w/v).

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Section: Discussionmentioning

confidence: 99%

Hydrogen sulfide production from elemental sulfur by Desulfovibrio desulfuricans in an anaerobic bioreactor

Escobar

Bravo

Hernández

et al. 2007

Biotech & Bioengineering

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“…Alternatively, the initial number of MB or SRB can be manipulated by shock treatments, e.g. a pH decrease from 8.0 to 7.0 (Omil et al, 1996), elevation (55°C; Visser et al, 1992) or drop (25°C; Omil et al, 1997c) of the operational temperature, and exposure to toxicants as chloroform (Visser et al, 1993b) or oxygen (Omil et al, 1997b). The thus created temporary unfavorable conditions for either AMB or ASRB allows the outgrowth of the other acetotrophic population.…”

Section: Discussionmentioning

confidence: 99%

Long-term competition between sulfate reducing and methanogenic bacteria in UASB reactors treating volatile fatty acids

Omil

Lens

Visser

et al. 1998

Biotechnol. Bioeng.

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The competition between acetate utilizing methane‐producing bacteria (MB) and sulfate‐reducing bacteria (SRB) was studied in mesophilic (30°C) upflow anaerobic sludge bed (UASB) reactors (upward velocity 1 m h−1; pH 8) treating volatile fatty acids and sulfate. The UASB reactors treated a VFA mixture (with an acetate:propionate:butyrate ratio of 5:3:2 on COD basis) or acetate as the sole substrate at different COD:sulfate ratios. The outcome of the competition was evaluated in terms of conversion rates and specific methanogenic and sulfidogenic activities. The COD:sulfate ratio was a key factor in the partitioning of acetate utilization between MB and SRB. In excess of sulfate (COD:sulfate ratio lower than 0.67), SRB became predominant over MB after prolonged reactor operation: 250 and 400 days were required to increase the amount of acetate used by SRB from 50 to 90% in the reactor treating, respectively, the VFA mixture or acetate as the sole substrate. The competition for acetate was further studied by dynamic simulations using a mathematical model based on the Monod kinetic parameters of acetate utilizing SRB and MB. The simulations confirmed the long term nature of the competition between these acetotrophs. A high reactor pH (±8), a short solid retention time (<150 days), and the presence of a substantial SRB population in the inoculum may considerably reduce the time required for acetate‐utilising SRB to outcompete MB. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 57: 676–685, 1998

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“…Using UASB reactors under methanogenic and sulfidogenic conditions, it was observed that in the absence of methanogenesis, SRB were unable to form sulfidogenic granules. However, SRB were able to attach to sludge granules when grown simultaneously with methanogens (Visser et al, 1993b). Other authors reported the ability of SRB to develop a biofilm over a plastic foil or 10-poly-methyl-metacrylate disks, even under aerobic or microaerophilic conditions (Ito et al, 2002;Santegoeds et al, 1998).…”

Section: Down-flow Fluidized Bed Reactor Performancementioning

confidence: 98%

Performance of a down‐flow fluidized bed reactor under sulfate reduction conditions using volatile fatty acids as electron donors

Celis-García

Razo-Flores

Monroy

2007

Biotech & Bioengineering

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The use of a down-flow fluidized bed (DFFB) reactor for the treatment of a sulfate-rich synthetic wastewater was investigated to obtain insight into the outcome of sulfate reduction in a biofilm attached to a plastic support under a down-flow regime. Fine low-density polyethylene particles were used as support for developing a biofilm within the reactor. The reactor treated a volatile fatty acids mixture of acetate or lactate, propionate, and butyrate at different chemical oxygen demand (COD) to sulfate ratios ranging from 1.67 to 0.67 (g/g). Organic loading rate changed from 2.5 to 5 g COD/L x day and sulfate loading rate increased from 1.5 to 7.3 g SO(4) (2-)/L x day. At the beginning of continuous operation, methanogenesis was the predominant process; however, after 187 days, sulfate reduction became the main ongoing biological process. After 369 days, a COD removal of 93% and a sulfate removal of 75% were reached. Total sulfide concentrations in the reactor ranged from 105, when the reactor was mainly methanogenic, to around 1,215 mg/L at the end of the experiment. The high sulfide concentrations did not affect the performance of the reactor. Results demonstrated that the configuration of the DFFB reactor was suitable for the anaerobic treatment of sulfate-rich wastewater.

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Granulation and immobilisation of methanogenic and sulfate-reducing bacteria in high-rate anaerobic reactors

Cited by 51 publications

References 22 publications

Hydrogen sulfide production from elemental sulfur by Desulfovibrio desulfuricans in an anaerobic bioreactor

Hydrogen sulfide production from elemental sulfur by Desulfovibrio desulfuricans in an anaerobic bioreactor

Long-term competition between sulfate reducing and methanogenic bacteria in UASB reactors treating volatile fatty acids

Performance of a down‐flow fluidized bed reactor under sulfate reduction conditions using volatile fatty acids as electron donors

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