Granulocyte macrophage colony stimulating factor (GM-CSF), the critical intermediate of inflammation-induced fetal membrane weakening, primarily exerts its weakening effect on the choriodecidua rather than the amnion

Sharma, Anudeepa; Kumar, Deepak; Moore, Robert M.; Deshmukh, Aditi R.; Mercer, Brian M.; Mansour, Joseph M.; Moore, John J.

doi:10.1016/j.placenta.2019.10.003

Cited by 9 publications

(11 citation statements)

References 54 publications

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“…Several models of inflammation have been utilized to examine the effect of ALA on the expression levels of MMP-1, MMP-2, MMP-8, and TIMP [ 72 , 73 ]. ALA’s anti-inflammatory and antioxidant effects in inflammation models have revealed that the protective effect is produced by lowering MMP-1, MMP-2, and MMP-8 expression while enhancing TIMP-1 activity [ 74 , 75 ]. Several of the 26 recognized MMPs have been extensively studied in inflammatory diseases and cancer.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Alpha-Lipoic Acid against 5-Fluorouracil-Induced Gastrointestinal Mucositis in Rats

et al. 2022

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Alpha-lipoic acid (ALA) is extensively utilized in multivitamin formulas and anti-aging products. The purpose of this study was to investigate the potential protective benefits of ALA on 5-fluorouracil (5-FU)-induced gastrointestinal mucositis in Wistar albino rats. Tissues from the stomach, small intestine, and large intestine were excised, and blood sera were obtained to identify biochemical indices such as TNF-α, IL-1β, MDA, GPx, SOD, MMP-1, -2, -8, and TIMP-1. A histopathological study was also performed. The results revealed mucositis-elevated TNF-, IL-1, MDA, MMP-1, -2, -8, and TIMP-1 levels in both tissues and sera, and these values dropped dramatically following ALA treatment. Reduced SOD and GPx activities in mucositis groups were reversed in ALA-treated groups. The damage produced by mucositis in the stomach and small intestine regressed in the ALA-treated group, according to histopathological evaluation. Consequently, the implementation of ALA supplementation in 5-FU therapy may act as a protective intervention for cancer patients with gastrointestinal mucositis. In light of the findings, ALA, a food-derived antioxidant with pleiotropic properties, may be an effective treatment for 5-FU-induced gastrointestinal mucositus, and prevent oxidative stress, inflammation, and tissue damage in cancer patients receiving 5-FU therapy.

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Section: Discussionmentioning

confidence: 99%

Protective Effects of Alpha-Lipoic Acid against 5-Fluorouracil-Induced Gastrointestinal Mucositis in Rats

et al. 2022

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“…Use of activated or resting Mφ-conditioned media on CTBs of the fetal membranes induced changes in gene expression in leukocyte trafficking, adhesion, and cytokinerelated genes, all of which was independent of changes to DNA methylation and thus likely not due to epigenetic regulation [95]. Recently, experiments performed on separated choriodecidua and amnion found that conditioned medium generated from choriodecidua treated with GM-CSF induced weakening of the amnion while treatment of the amnion with GM-CSF alone did not, suggesting that factors secreted from GM-CSF-stimulated choriodecidua result in weakening of the amnion [69]. Similar experiments performed on separated choriodecidua and amnion found that conditioned medium generated from choriodecidua containing IL-1α and β induced production of human beta defensin 2 from amnion [96].…”

Section: Methods To Study Paracrine Signaling In Fetal Membranesmentioning

confidence: 99%

“…Growth factors play important roles in regulation of fetal size and communication between layers during PPROM, and can be stored in regions of ECM, which would likely be released upon degradation of ECM [11] and in response to cellular senescence signals [68]. Key growth factors within the fetal membranes include granulocyte-M colony stimulating factor (GM-CSF) [64,69] (partially responsible for membrane weakening targeting the choriodecidua), vascular endothelial growth factor [70] (having a large role in regulation of amniotic fluid volume), and insulin-like growth factor (implicated in amnion epithelium proliferation [71] and associated with PPROM [72]).…”

Section: Pattern Recognition Receptors (Prrs)mentioning

confidence: 99%

Leveraging bioengineering to assess cellular functions and communication within human fetal membranes

Eastman

Noble

Aronoff

2020

The Journal of Maternal-Fetal & Neonatal Medicine

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The fetal membranes enclose the growing fetus and amniotic fluid. Preterm prelabor rupture of fetal membranes is a leading cause of preterm birth. Fetal membranes are composed of many different cell types, both structural and immune. These cells must coordinate functions for tensile strength and membrane integrity to contain the growing fetus and amniotic fluid. They must also balance immune responses to pathogens with maintaining maternal-fetal tolerance. Perturbation of this equilibrium can lead to preterm premature rupture of membranes without labor. In this review, we describe the formation of the fetal membranes to orient the reader, discuss some of the common forms of communication between the cell types of the fetal membranes, and delve into the methods used to tease apart this paracrine signaling within the membranes, including emerging technologies such as organ-on-chip models of membrane immunobiology.Leveraging Bioengineering to Assess Cellular Functions and Communication Within Human Fetal Membranes

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“…Additional in vitro studies have also shown GM-CSF increases the concentration of matrix metallopeptidases (MMPs) and decreases their inhibitors, tissue inhibitor metallopeptidases (TIMPs). 14 MMPs, enzymes which have been shown to weaken the fetal membranes, are elevated in the amniotic fluid and fetal serum with preterm premature rupture of membranes (PPROM), while their inhibitors, TIMPs, are decreased. 15,16 Therefore, GM-CSF plays a central role in amniotic membrane rupture by increasing the ratio of MMPs to TIMPs.…”

Section: Backg Rou N Dmentioning

confidence: 99%

“…Treatment with TNF and an antibody to GM‐CSF prevented this effect, indicating GM‐CSF is a key intermediate in membrane weakening. Additional in vitro studies have also shown GM‐CSF increases the concentration of matrix metallopeptidases (MMPs) and decreases their inhibitors, tissue inhibitor metallopeptidases (TIMPs) 14 . MMPs, enzymes which have been shown to weaken the fetal membranes, are elevated in the amniotic fluid and fetal serum with preterm premature rupture of membranes (PPROM), while their inhibitors, TIMPs, are decreased 15,16 .…”

Section: Introductionmentioning

confidence: 99%

Granulocyte‐macrophage colony‐stimulating factor initiates amniotic membrane rupture and preterm birth in a mouse model

Nold

Esteves

Jensen

et al. 2021

American J Rep Immunol

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Objective: Preterm premature rupture of membranes is associated with 30% of all preterm births. The weakening of amniotic membranes is associated with an increase in matrix metallopeptidases (MMPs) along with a decrease in their inhibitors, tissue inhibitor metallopeptidases (TIMPs). Additionally, granulocyte-macrophage colonystimulating factor (GM-CSF) has been shown to weaken fetal membranes in-vitro.We hypothesize pregnant mice treated with GM-CSF lead to increased MMPs:TIMPs resulting in membrane rupture and preterm birth.Study Design: Pregnant CD-1 mice on gestational day 17 received either an intrauterine injection of GM-CSF or vehicle control. A second series of mice were administered an intrauterine injection of Lipopolysaccharide along with either anti-mouse GM-CSF or control antibody. Mice were evaluated for rupture of membranes and/or preterm birth and the uterus, amniotic fluid, and serum were collected for analysis.Results: 87.5% of GM-CSF mice exhibited evidence of membrane rupture or preterm birth, compared with 0% in control mice (p < .001). Treatment with GM-CSF decreased the expression of TNFα (p < .05) while increasing the ratio of MMP2:TIMP1

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Granulocyte macrophage colony stimulating factor (GM-CSF), the critical intermediate of inflammation-induced fetal membrane weakening, primarily exerts its weakening effect on the choriodecidua rather than the amnion

Cited by 9 publications

References 54 publications

Protective Effects of Alpha-Lipoic Acid against 5-Fluorouracil-Induced Gastrointestinal Mucositis in Rats

Protective Effects of Alpha-Lipoic Acid against 5-Fluorouracil-Induced Gastrointestinal Mucositis in Rats

Leveraging bioengineering to assess cellular functions and communication within human fetal membranes

Granulocyte‐macrophage colony‐stimulating factor initiates amniotic membrane rupture and preterm birth in a mouse model

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