Granulocyte–macrophage colony-stimulating factor-containing medium treatment after thawing improves blastocyst-transfer outcomes in the frozen- thawed blastocyst-transfer cycle

Okabe-Kinoshita, Miki; Kobayashi, T; Shioya, Masashi; Sugiura, Tomoharu; Fujita, Maki; Takahashi, Keiichi

doi:10.1007/s10815-022-02493-1

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…Detailed ovarian stimulation protocols have been previously reported. 19 All patients underwent ovarian stimulation based on serum anti‐Müllerian hormone (AMH) levels and follicle‐stimulating hormone (FSH) levels on day 3 of the menstrual cycle. Ovarian stimulation was performed using the mild stimulation protocol with clomiphene citrate (CC), gonadotropin‐releasing hormone (GnRH) antagonist protocol, or GnRH agonist protocol.…”

Section: Methodsmentioning

confidence: 99%

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The morphokinetics algorithm based on data from day 5 blastocyst transfer (KIDScoreD5 version 3) is also useful for embryo selection in day 6 blastocyst transfer

Shioya

Kobayashi

Sugiura³

et al. 2022

Reprod Medicine & Biology

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Purpose To analyze whether the morphokinetics algorithm based on data from day 5 blastocyst transfer (KIDScoreD5 version 3) can predict the pregnancy rate of both day 5 and day 6 blastocyst transfers. Methods The relationship between KIDScoreD5 and clinical pregnancy rate was evaluated using the Cochran–Armitage test and receiver‐operating characteristic (ROC) curve analysis. Results A positive correlation was observed between the KIDScoreD5 value and clinical pregnancy rate for both day 5 ( p = 0.0003) and day 6 blastocysts ( p = 0.0019) using the Cochrane–Armitage test. ROC curve analysis showed that the area under the curve (AUC) of KIDScoreD5 for clinical pregnancy was 0.627 (0.575–0.677, p < 0.0001) for day 5 blastocysts and 0.685 (0.571–0.780, p = 0.0009) for day 6 blastocysts. The combined analysis of both day 5 and day 6 blastocysts also showed an AUC of 0.680 (0.636–0.720, p < 0.0001), suggesting that it is possible to select embryos that are more likely to result in pregnancy. Conclusions KIDScoreD5 could predict pregnancy not only in day 5 blastocysts but also in day 6 blastocysts. When both day 5 and day 6 blastocysts are vitrified, embryo selection by KIDScoreD5 is possible with a high prediction ability of pregnancy.

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Section: Methodsmentioning

confidence: 99%

“…Detailed ovarian stimulation protocols have been previously reported 19 . All patients underwent ovarian stimulation based on serum anti‐Müllerian hormone (AMH) levels and follicle‐stimulating hormone (FSH) levels on day 3 of the menstrual cycle.…”

Section: Methodsmentioning

confidence: 99%

The morphokinetics algorithm based on data from day 5 blastocyst transfer (KIDScoreD5 version 3) is also useful for embryo selection in day 6 blastocyst transfer

Shioya

Kobayashi

Sugiura³

et al. 2022

Reprod Medicine & Biology

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Culture conditions in the IVF laboratory: state of the ART and possible new directions

Sciorio,

Rinaudo

2023

J Assist Reprod Genet

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Disruption of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero

Sosa

Drum

et al. 2023

Reproduction and Fertility

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The hypothesis that CSF2 plays a role in the preimplantation development of the bovine embryo was tested by evaluating consequences of inactivation of CSF2RA (the functional receptor in the embryo) for development of embryos in utero. CRISPR/Cas9 was used to alter sequences on exon 5 and intron 5 of CSF2RA, Control embryos were injected with Cas9 mRNA only. Embryos > 16 cells at day 5 after insemination were transferred to synchronized recipient females in groups of 7 to 24. Embryos were flushed from the uterus two days later. The proportion of recovered embryos that developed to the blastocyst stage was lower for knockout embryos (39%) than for control embryos (63%). RNA sequencing of individual morulae and blastocysts indicated a total of 27 (morula) or 15 (blastocyst) differentially-expressed genes (false discovery rate <0.05). Gene set enrichment analysis indicated that the knockout affected genes playing roles in several functions including cell signaling and glycosylation. It was concluded that signaling through CSF2RA is not obligatory for development of the bovine preimplantation embryo to the blastocyst stage but that CSF2 signaling does enhance the likelihood that the embryo can become a blastocyst and result in specific changes in gene expression.

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Granulocyte–macrophage colony-stimulating factor-containing medium treatment after thawing improves blastocyst-transfer outcomes in the frozen- thawed blastocyst-transfer cycle

Cited by 4 publications

References 31 publications

The morphokinetics algorithm based on data from day 5 blastocyst transfer (KIDScoreD5 version 3) is also useful for embryo selection in day 6 blastocyst transfer

The morphokinetics algorithm based on data from day 5 blastocyst transfer (KIDScoreD5 version 3) is also useful for embryo selection in day 6 blastocyst transfer

Culture conditions in the IVF laboratory: state of the ART and possible new directions

Disruption of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero

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