Grape juice concentrate prevents oxidative DNA damage in peripheral blood cells of rats subjected to a high-cholesterol diet

Abstract

The goal of the present study was to investigate whether subchronic treatment with grape juice concentrate is able to protect liver and peripheral blood cells against cholesterol-induced injury in rats. The effects of the grape juice concentrate treatment on histopathological changes, immunohistochemistry for cyclo-oxygenase-2 (COX-2), and basal and oxidative DNA damage induced by H 2 O 2 using a single-cell gel (comet) assay were evaluated. Male Wistar rats (n 18) were divided into three groups: group 1 -negative control; group 2 -cholesterol at 1 % (w/w) in their diet, treated for 5 weeks; group 3 -cholesterol at 1 % in their chow, treated for 5 weeks, and grape juice concentrate at 222 mg/d in their drinking-water in the final week only. The results indicated that the treatment with grape juice concentrate did not show remarkable differences regarding liver tissue in group 3 compared with group 2. However, grape juice concentrate was able to decrease oxidative DNA damage induced by H 2 O 2 in peripheral blood cells, as depicted by the tail moment results. COX-2 expression in the liver did not show statistically significant differences (P. 0·05) between groups. Taken together, the present results suggest that the administration of subchronic grape juice concentrate prevents oxidative DNA damage in peripheral blood cells.

Antimutagenic and antigenotoxic potential of grape juice concentrate in blood and liver of rats exposed to cadmium

“…It is well known that micronucleus formation is linked to increased production of ROS, inhibition of DNA repair, as well as DNA breakage (Hengstler et al 2003;Degrandi et al 2010;Hartwig 2010;Bernhoft 2013). According to Aguiar et al (2011), grape juice polyphenolic compounds were able to limit oxidative DNA damage promoted by free radicals. Our results also demonstrated that grape juice concentrate G8000™ was able to prevent oxidative DNA damage in liver cells induced by hydrogen peroxide in vitro.…”

“…The chemical characterization of grape juice concentrate was performed in a previous study conducted by our research group (Aguiar et al 2011). The following compounds were identified: dimethoxyflavylium, disaccharide, fatty acids (palmitic and linoleic acids), and flavonoids (kaempferol-galactoside, peonidinglucoside, malvidin-glucoside, petunidin 3-O-acetylglucoside, peonidin 3-p-coumaroylglucoside, and malvidin 3-O-p-coumaroylglucoside).…”

Cholesterol overload impairing cerebellar function: The promise of natural products

“…[130], citrus peel [131], Lycium ruthenicum anthocyanins [132], arti choke leaf extract [133], Ficus microcarpa (L.) [134], grape juice [135], Ficus racemosa [136], and Hibiscus sabadariffa extract [137].…”

Oral Concentrated Grape Juice Suppresses Expression of NF-kappa B, TNF-α and iNOS in Experimentally Induced Colorectal Carcinogenesis in Wistar Rats

“…in rat colon after Azoxymethane (AOM). Histopathologic alterations in rat colon of groups submitted to grape juice intake; C: before and after AOM, 1% grape juice intake before and after AOM; D: 1% grape juice intake after AOM, E: 2% grape juice intake before and after AOM; F: 2% grape juice intake after AOM vegetable compounds and their molecular mechanisms of action in order to elucidate the mechanisms underlying the suppression of colorectal carcinogenesis (Aguiar et al, 2011). Resveratrol is a component found in the concentrated grape juice used in the present study and this compound has been shown to inhibit the three stages of carcinogenesis (Aggarwal et al, 2004).…”