Grape seed proanthocyanidin extract ameliorates dextran sulfate sodium-induced colitis through intestinal barrier improvement, oxidative stress reduction, and inflammatory cytokines and gut microbiota modulation

Sheng, Kangliang; Zhang, Guanghui; Sun, Ming; He, Shiman; Kong, Xiaowei; Wang, Jingmin; Zhu, Fenfang; Zha, Xiangdong; Wang, Yongzhong

doi:10.1039/d0fo01418d

Cited by 99 publications

(57 citation statements)

References 50 publications

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“…Under the naïve condition, Tph1 −/− mice had elevated levels of protective bacteria such as Lachnospiraceae and reduced levels of colitogenic bacteria such as Mucispirillum and Desulfovibrionaceae compared to DKO mice (56,57). Moreover, Dubosiella and Turicibacter that are increased during colitis (58,59) were significantly higher in the more colitic DKO and Atg7 IEC donors and recipients. Furthermore, the transfer of colitic or naïve DKO microbiota to antibiotic-treated mice was associated with increased inflammation severity.…”

Section: Discussionmentioning

confidence: 99%

Disruption of autophagy by increased 5-HT alters gut microbiota and enhances susceptibility to experimental colitis and Crohn’s disease

et al. 2021

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Section: Discussionmentioning

confidence: 99%

Disruption of autophagy by increased 5-HT alters gut microbiota and enhances susceptibility to experimental colitis and Crohn’s disease

et al. 2021

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“…An increasing number of studies demonstrated the beneficial effects of grape compounds and grape by-products in various chronic diseases, including IBD [ 23 , 24 ]. In mice with DSS-induced colitis, grape seed proanthocyanidin extract alleviates colitis symptoms by modulating inflammatory cytokines and oxidative stress, maintaining the intestinal barrier, and by improving the microbial community [ 25 ]. Furthermore, prophylactic consumption of polyphenol-rich grape pomace extracts exerts protective effects against DSS-induced colitis, by decreasing the inflammatory status at the colon level [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Dietary Grape Seed Meal Bioactive Compounds Alleviate Epithelial Dysfunctions and Attenuates Inflammation in Colon of DSS-Treated Piglets

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Bulgaru

Marin

et al. 2021

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Inflammatory Bowel Diseases (IBD) are chronic inflammations associated with progressive degradation of intestinal epithelium and impairment of the local innate immune response. Restoring of epithelial integrity and of the mucosal barrier function, together with modulation of inflammatory and innate immune markers, represent targets for alternative strategies in IBD. The aim of our study was to evaluate the effects of a diet including 8% grape seed meal (GSM), rich in bioactive compounds (polyphenols, polyunsaturated fatty acids (PUFAs), fiber) on the markers of colonic epithelial integrity, mucosal barrier function, pro-inflammatory, and innate immunity in DSS-treated piglets used as animal models of intestinal inflammation. Our results have demonstrated the beneficial effects of bioactive compounds from dietary GSM, exerted at three complementary levels: (a) restoration of the epithelial integrity and mucosal barrier reinforcement by modulation of claudins, Occludin (OCCL) and Zonula-1 (ZO-1) tight junction genes and proteins, myosin IXB (MYO9B) and protein tyrosine phosphatase (PTPN) tight junction regulators and mucin-2 (MUC2) gene; (b) reduction of pro-inflammatory MMP-2 (matrix metalloproteinase-2) and MMP-9 (matrix metalloproteinase-9) genes and activities; and (c) suppression of the innate immune TLR-2 (Toll-like receptor-2) and TLR-4 (Toll-like receptor-4) genes and attenuation of the expression of MyD88 (Myeloid Differentiation Primary Response 88)/MD-2 (Myeloid differentiation factor-2) signaling molecules. These beneficial effects of GSM could further attenuate the transition of chronic colitis to carcinogenesis, by modulating the in-depth signaling mediators belonging to the Wnt pathway.

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“…We therefore analyzed the microbial distribution. As previously reported, DSS boosted the genera Enterococcus [ 29 ], Turicibacter [ 30 ], Lactobacillus [ 15 ], Dubosiella [ 15 ], and Alloprevotella [ 31 , 32 ], most of which are also considered harmful bacteria in colitis [ 15 , 33 , 34 ]. These results suggested that the main contribution of DSS toward increasing bacterial richness and diversity was harmful bacteria.…”

Section: Discussionmentioning

confidence: 71%

“…Changes in the composition and function of the gut microbiota cause alterations in intestinal ECs and TJ proteins [ 12 , 13 , 14 ], both of which are responsible for controlling gut permeability. An increase in gut permeability is known to facilitate microbial translocation, perpetuating UC [ 15 ]. Therefore, exploiting drugs that synergize gut barrier protection and microbiota regulation could be a promising avenue for UC treatment.…”

Section: Introductionmentioning

confidence: 99%

The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis

et al. 2021

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The gut epithelium is a mechanical barrier that protects the host from the luminal microenvironment and interacts with the gut microflora, which influences the development and progression of ulcerative colitis (UC). Licochalcone A (LA) exerts anti-inflammatory effects against UC; however, whether it also regulates both the gut barrier and microbiota during colitis is unknown. The current study was conducted to reveal the regulatory effects of LA on the intestinal epithelium and gut microflora in C57BL/6 mice subjected to dextran sodium sulfate (DSS). Sulfasalazine (SASP) was used as the positive control. Results of clinical symptoms evaluation, hematoxylin, and eosin (H&E) staining, and enzyme-linked immunosorbent (ELISA) assays showed that LA significantly inhibited DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and gut inflammation. Additionally, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and immunohistochemical (IHC) analysis showed that LA maintained the integrity of the intestinal barrier by suppressing cell apoptosis and preserving the expression of tight junction (TJ) proteins. Notably, the optimal dose of LA for gut barrier preservation was low, while that for anti-inflammatory effects was high, indicating that LA might preserve gut barrier integrity via direct effects on the epithelial cells (ECs) and TJ proteins. Furthermore, 16S rRNA analysis suggested that the regulatory effect of LA on the gut microbiota differed distinctly according to dose. Correlation analysis indicated that a low dose of LA significantly modulated the intestinal barrier-associated bacteria as compared with a moderate or high dose of LA. Western blot (WB) analysis indicated that LA exhibited anti-UC activity partly by blocking the mitogen-activated protein kinase (MAPK) pathway. Our results further elucidate the pharmacological activity of LA against UC and will provide valuable information for future studies regarding on the regulatory effects of LA on enteric diseases.

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Grape seed proanthocyanidin extract ameliorates dextran sulfate sodium-induced colitis through intestinal barrier improvement, oxidative stress reduction, and inflammatory cytokines and gut microbiota modulation

Abstract: It is widely believed that grape seed proanthocyanidin extract (GSPE) exerts antioxidant and anti-inflammatory effects. Dietary supplementation with GSPE has been reported to alleviate colitis signs in mice, but the...

Cited by 99 publications

References 50 publications

Disruption of autophagy by increased 5-HT alters gut microbiota and enhances susceptibility to experimental colitis and Crohn’s disease

Disruption of autophagy by increased 5-HT alters gut microbiota and enhances susceptibility to experimental colitis and Crohn’s disease

Dietary Grape Seed Meal Bioactive Compounds Alleviate Epithelial Dysfunctions and Attenuates Inflammation in Colon of DSS-Treated Piglets

The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis

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