Grape Seed Procyanidin Extract Mediates Antineoplastic Effects against Lung Cancer via Modulations of Prostacyclin and 15-HETE Eicosanoid Pathways

Mao, Jenny T.; Smoake, Jane; Park, Heesung K.; Lu, Qing-Yi; Xue, Bingye

doi:10.1158/1940-6207.capr-16-0122

Cited by 35 publications

(29 citation statements)

References 27 publications

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“…The other two pathways that are involved in the formation of midchain HETEs are LOX and COX-2 enzymes. 5-LOX, 12-LOX, and 15-LOX enzymes metabolize AA to produce 5-, 12-, and 15-HETE, respectively (Porro et al, 2014;Radmark et al, 2015;Mao et al, 2016). In addition, it has been reported that minor quantities of midchain HETEs are produced by COX-2 enzyme (Bai and Zhu, 2008).…”

Section: Discussionmentioning

confidence: 99%

S-Enantiomer of 19-Hydroxyeicosatetraenoic Acid Preferentially Protects Against Angiotensin II-Induced Cardiac Hypertrophy

Shoieb

El‐Kadi

2018

Drug Metab Dispos

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We had recently demonstrated that the racemic mixture of 19-hydroxyeicosatetraenoic acid (19-HETE) protects against angiotensin II (Ang II)-induced cardiac hypertrophy. Therefore, the purpose of this study was to investigate whether the orenantiomer of 19-HETE confers cardioprotection against Ang II-induced cellular hypertrophy in RL-14 and H9c2 cells. Both cell lines were treated with vehicle or 10 M Ang II in the absence and presence of 20M 19()HETE or 19()HETE for 24 hours. Thereafter, the level of midchain HETEs was determined using liquid chromatography-mass spectrometry. Gene- and protein-expression levels were measured using real-time polymerase chain reaction and Western blot analysis, respectively. The results showed that both 19()HETE and 19()HETE significantly decreased the metabolite formation rate of midchain HETEs, namely 8-, 9-, 12-, and 15-HETE, compared with control group, whereas the level of 5-HETE was selectively decreased by enantiomer. Moreover, both 19()HETE and 19()HETE significantly inhibited the catalytic activity of CYP1B1 and decreased the protein expression of 5- and 12-lipoxygenase (LOX) as well as cyclo-oxygenase-2 (COX-2). Notably, the decrease in 15-LOX protein expression was only mediated by 19()HETE. Interestingly, both enantiomers protected against Ang II-induced cellular hypertrophy, as evidenced by a significant decrease in mRNA expression of /-myosin heavy chain ratio, atrial natriuretic peptide, and interleukins 6 and 8. Our data demonstrated that enantiomer of 19-HETE preferentially protected against Ang II-induced cellular hypertrophy by decreasing the level of midchain HETEs, inhibiting catalytic activity of CYP1B1, decreasing protein expression of LOX and COX-2 enzymes, and decreasing mRNA expression of IL-6 and IL-8.

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Section: Discussionmentioning

confidence: 99%

S-Enantiomer of 19-Hydroxyeicosatetraenoic Acid Preferentially Protects Against Angiotensin II-Induced Cardiac Hypertrophy

Shoieb

El‐Kadi

2018

Drug Metab Dispos

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“…GSE is mainly used as a health food supplement to improve cardiovascular health [ 3 ]. Preclinical studies have shown antineoplastic effects of GSE against a variety of cancers, including lung cancer [ 4 – 9 ]. We recently reported that modulations of oncogenic microRNA (miRNA) or oncomir miR-19a/b, contributed to the antineoplastic properties of GSE against non-small cell lung cancer (NSCLC) and bronchial premalignant cells [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Grape seed procyanidin extract against lung cancer: the role of microrna-106b, bioavailability, and bioactivity

Xue

Lu²,

Massie

et al. 2018

Oncotarget

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MiR-106b is an oncomir and a potential target for anti-cancer therapy. We hypothesize that grape seed procyanidin extract (GSE) exerts antineoplastic effects on lung cancer through modulations of miR-106b and its downstream target. We found that GSE significantly down-regulated miR-106b in a variety of lung neoplastic cells and increased cyclin-dependent kinase inhibitor 1A (CDKN1A) mRNA and protein (p21) levels. Transfection of miR-106b mimics reversed the up-regulations of CDKN1A mRNA and p21, abrogated the GSE induced anti-proliferative and anti-invasive properties in lung cancer cells. Oral gavage of leucoselect phytosome (LP), a standardized GSE to athymic nude mice down-regulated MIR106B mRNA and miR-106b expressions, and increased CDKN1A mRNA expression in tumor xenografts, correlating to significant reduction of tumor growth. To assess bioavailability, GSE and metabolites in plasma levels, between 60–90 minutes after gavage of LP were measured by LC/MS at treatment week 4 and 8. A novel bioactivity assay was also developed using lung homogenates from treated mice co-cultured with human lung cancer cells. LP-treated mouse lung homogenates significantly reduced proliferations of various lung cancer cells. Our findings reveal novel antineoplastic mechanisms by GSE, further define the pharmacokinetics and pharmacodynamics of LP, and support the continued investigation of LP against lung cancer.

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“…En este estudio, solo el número de frutas estuvo según lo sugerido. A pesar de, toda la comunicación social que existe a nivel mundial para el consumo de frutas por su alto contenido de polifenoles que contribuyen como agente protector antioxidante, a reducir riesgos de cáncer (Fu et al, 2011;Li et al, 2016) y las verduras igualmente, ante su protección anticancerígena y de enfermedades crónicas (Zhang et al, 2015), al menos se observó en este estudio gran consumo de una de las frutas como es la uva (Vaid & Katiyar, 2015;Mao et al, 2016) y del tomate como vegetal (Trejo et al, 2013;Aizawa et al, 2016) quienes son reconocidos por su gustación, más que el valor nutricional.…”

Section: Discussionunclassified

Consumo Nutricional De Frutas Y Vegetales en Ica, Perú: Estudio Poblacional Piloto

Pérez¹,

Castillo-Romero²,

Chávez-Espinoza³

et al. 2019

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Keywords: fruits-natural products-quality of life-vegetables-well-being The sustainable acquisition of natural sources in minerals and vitamins constitutes a guarantee for public health. The purpose of study was to evaluate the nutritional consumption of fruits and vegetables in a pilot population of Ica, Peru. An unstructured observation was made from a distance where it was selected, two points of sales referring to consumption of fruits and vegetables. Through a non-probabilistic sampling for convenience, it was measured every 15 days (Saturday) in two schedules (08:30-10:30 and 14:30-16:30), the consumption according to type of fruit while, the frequency was checked by the number of people that acquired the fruits and vegetables. It was observed that, the vegetables in the morning section corresponded to preferences for consumption. However, the number of people was increasing during the evening time intervals where it was indicated by wholesalers, the decrease in the monetary cost before the possibility the do not return or store fruits and vegetables. It was concluded that there was consumption by the pilot population, which was an indicator of concern for health welfare and a healthy lifestyle.

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Grape Seed Procyanidin Extract Mediates Antineoplastic Effects against Lung Cancer via Modulations of Prostacyclin and 15-HETE Eicosanoid Pathways

Cited by 35 publications

References 27 publications

S-Enantiomer of 19-Hydroxyeicosatetraenoic Acid Preferentially Protects Against Angiotensin II-Induced Cardiac Hypertrophy

S-Enantiomer of 19-Hydroxyeicosatetraenoic Acid Preferentially Protects Against Angiotensin II-Induced Cardiac Hypertrophy

Grape seed procyanidin extract against lung cancer: the role of microrna-106b, bioavailability, and bioactivity

Consumo Nutricional De Frutas Y Vegetales en Ica, Perú: Estudio Poblacional Piloto

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