Graphene-Augmented Nanofiber Scaffolds Trigger Gene Expression Switching of Four Cancer Cell Types

Kazantseva, Jekaterina; Ivanov, Roman; Gasik, Michael; Neuman, Toomas; Hussainova, Irina

doi:10.1021/acsbiomaterials.8b00228

Cited by 11 publications

(12 citation statements)

References 49 publications

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“…On the one hand, the biomimetic nano-micron morphology contributes to the good cell functions. Although there is no report on cancer cells cultured in nano-microfibrous scaffolds, a few previous studies confirmed the suitability of nanofibrous scaffolds for cancer cell growth and survival (Sims-Mourtada et al 2014;Kazantseva et al 2018;Bae et al 2011), which indicates that both normal and cancerous tissues are responsive to ECMlike microenvironments. Here, we combine nanofibers with microfibers to provide a more elaborate ECMlike microenvironment than single nanofibers or microfibers.…”

Section: Discussionmentioning

confidence: 99%

Incorporating graphene oxide into biomimetic nano-microfibrous cellulose scaffolds for enhanced breast cancer cell behavior

et al. 2020

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The impact of graphene oxide (GO) on normal cells has been widely investigated. However, much less is known on its effect on cancer cells. Herein, GO nanosheets were incorporated into electrospun cellulose acetate (CA) microfibers. The GOincorporated CA (GO/CA) microfibers were combined with bacterial cellulose (BC) nanofibers via in situ biosynthesis to obtain the nano-microfibrous scaffolds. The GO/CA-BC scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS). The GO/ CA-BC scaffolds were used for breast cancer cell culture to evaluate the effect of GO on cancer cell behavior. Fluorescence images revealed large multicellular clusters on the surface of GO/CA-BC scaffolds. Compared to the bare CA-BC scaffold, the GO/ CA-BC scaffolds not only showed enhanced mechanical properties but also improved cell proliferation. It is expected that the GO/CA-BC scaffolds would provide a suitable microenvironment for the culture of cancer cells which is necessary for drug screening and cell biology study.

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Section: Discussionmentioning

confidence: 99%

Incorporating graphene oxide into biomimetic nano-microfibrous cellulose scaffolds for enhanced breast cancer cell behavior

et al. 2020

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“…Growth on highly aligned graphene fibers changed the morphology from flat to a more rounded shape as the cells enveloped the fibers. In addition, increased gene expression of promigratory and pro-invasion markers were observed [172]. This represents a potential system to examine the more migratory or more metastatic NB cells, and develop therapeutics aimed at effectively inhibiting those cells.…”

Section: Hydrogels and Scaffolds For 3d Tumor Growthmentioning

confidence: 98%

“…Scaffold based studies have also been used for biomechanical modeling. One scaffold-based study used graphene-augmented nanofiber scaffolds to determine the impact of an "out-of-comfort" nanobiomechanical environment for NB cells [172]. Growth on highly aligned graphene fibers changed the morphology from flat to a more rounded shape as the cells enveloped the fibers.…”

Section: Hydrogels and Scaffolds For 3d Tumor Growthmentioning

confidence: 99%

Developing preclinical models of neuroblastoma: driving therapeutic testing

Ornell

Coburn

2019

BMC biomed eng

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Despite advances in cancer therapeutics, particularly in the area of immuno-oncology, successful treatment of neuroblastoma (NB) remains a challenge. NB is the most common cancer in infants under 1 year of age, and accounts for approximately 10% of all pediatric cancers. Currently, children with highrisk NB exhibit a survival rate of 40-50%. The heterogeneous nature of NB makes development of effective therapeutic strategies challenging. Many preclinical models attempt to mimic the tumor phenotype and tumor microenvironment. In vivo mouse models, in the form of genetic, syngeneic, and xenograft mice, are advantageous as they replicated the complex tumor-stroma interactions and represent the gold standard for preclinical therapeutic testing. Traditional in vitro models, while high throughput, exhibit many limitations. The emergence of new tissue engineered models has the potential to bridge the gap between in vitro and in vivo models for therapeutic testing. Therapeutics continue to evolve from traditional cytotoxic chemotherapies to biologically targeted therapies. These therapeutics act on both the tumor cells and other cells within the tumor microenvironment, making development of preclinical models that accurately reflect tumor heterogeneity more important than ever. In this review, we will discuss current in vitro and in vivo preclinical testing models, and their potential applications to therapeutic development.

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“…Micro-and nano-fibers created by electrospinning guarantee high porosity of the structures and favor neuroblastoma cell proliferation and adhesion, while promoting neurite out-growth (60). The usefulness of the highly aligned graphene-augmented inorganic nanofiber (GAIN) scaffolds for biomedical cancer research has also been proven for several tumor cell types including neuroblastoma (61). Although they do not allow tumor-like 3D cell organization entirely, these scaffolds open an opportunity for a fast and highly reproducible validation of anti-cancer drugs oriented toward the modulation of cell migration.…”

Section: Cast In Vitro 3d Models For Studying Neuroblastomamentioning

confidence: 99%

Emerging Neuroblastoma 3D In Vitro Models for Pre-Clinical Assessments

Corallo

Frabetti²,

Candini³

et al. 2020

Front. Immunol.

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The potential of tumor three-dimensional (3D) in vitro models for the validation of existing or novel anti-cancer therapies has been largely recognized. During the last decade, diverse in vitro 3D cell systems have been proposed as a bridging link between two-dimensional (2D) cell cultures and in vivo animal models, both considered gold standards in pre-clinical settings. The latest awareness about the power of tailored therapies and cell-based therapies in eradicating tumor cells raises the need for versatile 3D cell culture systems through which we might rapidly understand the specificity of promising anti-cancer approaches. Yet, a faithful reproduction of the complex tumor microenvironment is demanding as it implies a suitable organization of several cell types and extracellular matrix components. The proposed 3D tumor models discussed here are expected to offer the required structural complexity while also assuring cost-effectiveness during pre-selection of the most promising therapies. As neuroblastoma is an extremely heterogenous extracranial solid tumor, translation from 2D cultures into innovative 3D in vitro systems is particularly challenging. In recent years, the number of 3D in vitro models mimicking native neuroblastoma tumors has been rapidly increasing. However, in vitro platforms that efficiently sustain patient-derived tumor cell growth, thus allowing comprehensive drug discovery studies on tailored therapies, are still lacking. In this review, the latest neuroblastoma 3D in vitro models are presented and their applicability for a more accurate prediction of therapy outcomes is discussed.

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Graphene-Augmented Nanofiber Scaffolds Trigger Gene Expression Switching of Four Cancer Cell Types

Cited by 11 publications

References 49 publications

Incorporating graphene oxide into biomimetic nano-microfibrous cellulose scaffolds for enhanced breast cancer cell behavior

Incorporating graphene oxide into biomimetic nano-microfibrous cellulose scaffolds for enhanced breast cancer cell behavior

Developing preclinical models of neuroblastoma: driving therapeutic testing

Emerging Neuroblastoma 3D In Vitro Models for Pre-Clinical Assessments

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