Graphene based pipette tip solid phase extraction of marine toxins in shellfish muscle followed by UPLC–MS/MS analysis

Tetracyclines abuse has frequently occurred in aquaculture against bacteria, rickettsiae, spirochetes, and mycoplasmas. In this study, a high‐throughput sample preparation method was developed using 96‐well plate solid‐phase extraction (p‐SPE) and the extract was analyzed by ultra‐performance liquid chromatography tandem mass spectrometry (UPLC‐MS/MS). The experimental conditions were optimized such that the pH is 4, the eluting solvent is methanol (2 mL), and the sorbent is hydrophilic‐lipophilic balance (HLB) microsphere. The whole protocol was validated, and it showed that the tetracyclines were linear with correlation coefficients ≥ 0.9990, precision and accuracy (RSD%) in 3.9–6.1%, and mean recoveries of 88.6–103.6%. To exhibit the potential of 96‐well p‐SPE as a routine tool for inspection and quarantine, fresh aquatic samples were tested, and among which positive samples were observed. This method was demonstrated to be promising for the purification and enrichment of tetracyclines with reduced time and labor, and indeed practically and particularly suitable for widespread tetracyclines analysis.

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“…The following performance studies were carried out, which are linearity, LOD, LOQ, intra‐ and inter‐day precision, recovery, and stability .…”

Section: Resultsmentioning

confidence: 99%

High‐throughput 96‐well solid‐phase extraction for preparation of tetracycline followed by liquid chromatography and mass spectrometry analysis

Kang

Wang

et al. 2018

Electrophoresis

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“…The developed SPE-GO-MPPS isolated metal ions from complex matrices with less interference. Excellent sensitivity (145)(146)(147)(148)(149)(150)(151)(152)(153)(154)(155)(156)(157)(158)(159)(160)(161)(162) ng L -1 ), great enrichment factor (325), and good recovery (99-103%) were obtained. Selectivity was considered in the presence of various coexisting ions with > 90% extraction recovery.…”

Section: Applications Of Graphene-based Spe In Biological Sample Analmentioning

confidence: 94%

“…MSPE based on Fe 3 O 4 @SiO 2 -G was used to isolate phthalate extracts from soybean milk [112]. High adsorption Pipette tip SPE based on graphene was used to extract lipophilic marine toxic analytes from fish tissues [146]. Graphene showed high extraction recovery due to high surface area, thermal stability and compatibility with different solvents.…”

Section: Applications Of Graphene-based Spe In Biological Sample Analmentioning

confidence: 99%

Graphene-Based Materials as Solid Phase Extraction Sorbent for Trace Metal Ions, Organic Compounds, and Biological Sample Preparation

Ibrahim

Nodeh

Sanagi

2015

Critical Reviews in Analytical Chemistry

113

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Graphene is a new carbon-based material that is of interest in separation science. Graphene has extraordinary properties including nano size, high surface area, thermal and chemical stability, and excellent adsorption affinity to pollutants. Its adsorption mechanisms are through non-covalent interactions (π-π stacking, electrostatic interactions, and H-bonding) for organic compounds and covalent interactions for metal ions. These properties have led to graphene-based material becoming a desirable adsorbent in a popular sample preparation technique known as solid phase extraction (SPE). Numerous studies have been published on graphene applications in recent years, but few review papers have focused on its applications in analytical chemistry. This article focuses on recent preconcentration of trace elements, organic compounds, and biological species using SPE-based graphene, graphene oxide, and their modified forms. Solid phase microextraction and micro SPE (µSPE) methods based on graphene are discussed.

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“…Extraction strategies, such as solid-liquid extraction (SLE) (Borràs, Companyó, & Guiteras, 2011), solidphase extraction (SPE) (Shen et al, 2013) and QuEChERS methodology (Quick, Easy, Cheap, Effective, Rugged and Safe) (Lopes, Reyes, Romero-González, Vidal, & Frenich, 2012) have been widely employed to achieve extraction and cleanup during the determination of multi-residue and multiclass compounds in different matrix. Matrix solid phase dispersion (MSPD) has been established as an effective technique for the analysis of exogenous (i.e., drugs, pollutants, pesticides) and endogenous (i.e., aflatoxins, etc.)…”

Section: Introductionmentioning

confidence: 99%

Analysis of trace levels of sulfonamides in fish tissue using micro-scale pipette tip-matrix solid-phase dispersion and fast liquid chromatography tandem mass spectrometry

et al. 2016

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Graphene based pipette tip solid phase extraction of marine toxins in shellfish muscle followed by UPLC–MS/MS analysis

Cited by 68 publications

References 27 publications

High‐throughput 96‐well solid‐phase extraction for preparation of tetracycline followed by liquid chromatography and mass spectrometry analysis

High‐throughput 96‐well solid‐phase extraction for preparation of tetracycline followed by liquid chromatography and mass spectrometry analysis

Graphene-Based Materials as Solid Phase Extraction Sorbent for Trace Metal Ions, Organic Compounds, and Biological Sample Preparation

Analysis of trace levels of sulfonamides in fish tissue using micro-scale pipette tip-matrix solid-phase dispersion and fast liquid chromatography tandem mass spectrometry

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