Graphene Oxide as a Pathogen‐Revealing Agent: Sensing with a Digital‐Like Response

Morales‐Narváez, Eden; Hassan, Abdelrahim H. A.; Merkoçi, Arben

doi:10.1002/ange.201307740

Cited by 14 publications

(7 citation statements)

References 50 publications

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“…803 CdSe/ ZnS QDs were functionalized with E. coli antibodies and printed in microarrays. In the absence of E. coli, GO interacts with the probes through π−π stacking, thereby quenching the QD PL.…”

Section: Sensorsmentioning

confidence: 99%

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Spillmann²,

et al. 2016

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Luminescent semiconductor quantum dots (QDs) are one of the more popular nanomaterials currently utilized within biological applications. However, what is not widely appreciated is their growing role as versatile energy transfer (ET) donors and acceptors within a similar biological context. The progress made on integrating QDs and ET in biological configurations and applications is reviewed in detail here. The goal is to provide the reader with (1) an appreciation for what QDs are capable of in this context, (2) how this field has grown over a relatively short time span, and, in particular, (3) how QDs are steadily revolutionizing the development of new biosensors along with a myriad of other photonically active nanomaterial-based bioconjugates. An initial discussion of QD materials along with key concepts surrounding their preparation and bioconjugation is provided given the defining role these aspects play in the QDs ability to succeed in subsequent ET applications. The discussion is then divided around the specific roles that QDs provide as either Förster resonance energy transfer (FRET) or charge/electron transfer donor and/or acceptor. For each QD-ET mechanism, a working explanation of the appropriate background theory and formalism is articulated before examining their biosensing and related ET utility. Other configurations such as incorporation of QDs into multistep ET processes or use of initial chemical and bioluminescent excitation are treated similarly. ET processes that are still not fully understood such as QD interactions with gold and other metal nanoparticles along with carbon allotropes are also covered. Given their maturity, some specific applications ranging from in vitro sensing assays to cellular imaging are separated and discussed in more detail. Finally a perspective on how this field will continue to evolve is provided.

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Section: Sensorsmentioning

confidence: 99%

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Spillmann²,

et al. 2016

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“…Full details on the operational concept of GO as a pathogen-revealing agent has been previously reported by our research group. 36 Herein spots for individual assays made of QD-BC were functionalized with biotinylated antibodies against Escherichia coli (E. coli) through streptavidin-biotin affinity (as mentioned above the utilized QDs are coated with streptavidin). Phosphate-buffered saline (PBS) and tap water were used as matrixes to perform this novel immunoassay.…”

Section: Acs Nanomentioning

confidence: 99%

“…Bacteria strains were obtained as previously reported. 36 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 19 interactions by drop casting 3 µL of antibodies concentrated at 400 µg mL -1 onto the studied QD-BC spots. Antibodies were diluted in phosphate-buffered saline (PBS) supplemented with Tween 20 at 0.5% (v/v) and bovine serum albumin at 1% (w/v).…”

Section: Reagentsmentioning

confidence: 99%

Nanopaper as an Optical Sensing Platform

et al. 2015

Self Cite

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Bacterial cellulose nanopaper (BC) is a multifunctional material known for numerous desirable properties: sustainability, biocompatibility, biodegradability, optical transparency, thermal properties, flexibility, high mechanical strength, hydrophilicity, high porosity, broad chemical-modification capabilities and high surface area. Herein, we report various nanopaper-based optical sensing platforms and describe how they can be tuned, using nanomaterials, to exhibit plasmonic or photoluminescent properties that can be exploited for sensing applications. We also describe several nanopaper configurations, including cuvettes, plates and spots that we printed or punched on BC. The platforms include a colorimetric-based sensor based on nanopaper containing embedded silver and gold nanoparticles; a photoluminescent-based sensor, comprising CdSe@ZnS quantum dots conjugated to nanopaper; and a potential up-conversion sensing platform constructed from nanopaper functionalized with NaYF4:Yb(3+)@Er(3+)&SiO2 nanoparticles. We have explored modulation of the plasmonic or photoluminescent properties of these platforms using various model biologically relevant analytes. Moreover, we prove that BC is and advantageous preconcentration platform that facilitates the analysis of small volumes of optically active materials (∼4 μL). We are confident that these platforms will pave the way to optical (bio)sensors or theranostic devices that are simple, transparent, flexible, disposable, lightweight, miniaturized and perhaps wearable.

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“…The performance of FRET can be enhanced by maximizing the overlap of the donor emission spectrum and absorbance spectra of the acceptor 20 . Basically, the most frequently used immunosensing based-FRET scenarios consider a non-competitive (e.g., sandwich model and direct interaction) 18 or competitive interaction 17 .…”

Section: Introductionmentioning

confidence: 99%

Toward a nanopaper-based and solid phase immunoassay using FRET for the rapid detection of bacteria

Heli

Ajji

2020

Sci Rep

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in this study, we propose a novel sensitive solid-based immunosensor developed on a plasmonic nanopaper platform for the detection of Escherichia coli (E. coli) bacteria. this plasmonic nanopaper that comprises of carboxylated bacterial cellulose (cBc) impregnated with gold nanoparticles (Aunp-cBc), employed as a quencher and a sustainable functionalized platform to be conjugated with protein A. thus, the conjugated protein A allows the aligned linkage of eAb-QD (anti-E. coli conjugated quantum dot) and eAb-Af (anti-E. coli conjugated Alexa Fluor 488). Interestingly, once E. coli was captured by the Aunp-cBc/eAb-QD or Aunp-cBc/eAb-Af, the energy transfer from the QD or Alexa Fluor fluorophores is triggered due to the conformational change in the antibody structure and this, in turn, causes a decrease in the distance between fluorophores and the quencher nanopaper and, therefore diminishing their photoluminescence. the immunosensors performed successfully to recognize E. coli at concentrations as low as 50 CFU mL −1 in the standard buffer. The examined functionality of the immunosensors in a real matrix such as chicken extract and lettuce juice demonstrated a highly efficient response while QD is the main fluorophore with a limit of detection around 100 CFU mL −1. During the last decades, the outbreak of foodborne diseases has seriously threatened people lives worldwide and led to a considerable number of hospitalizations and even deaths 1. Indeed, the incidence of foodborne infection is a result of ingesting food contaminated by foodborne pathogens such as bacteria, viruses, and parasites. To prevent its subsequence, precise monitoring and early recognition of pathogen would be the primary step to be ensured about the safety of our food 2. Upon comparing various categories of biosensors developed for pathogen detection, immunosensors have attracted plenty of attention due to their flexibility and compatibility in numerous transducer techniques such as electrochemical (voltammetry, impedancemetry, potentiometry), optical (fluorescence, SPR) and mass-based 3-5. The excellence of an immunosensor is directly dominated by the affinity binding of the antigen-antibody that this makes it uniquely sensitive and selective toward a specific target 6. Being fast, selective and sensitive, low-priced, in-situ and user-friendly, immunosensors can fully address the challenges associated with traditional bacteria detection approaches, such as; costly analysis, long processing time, and needs in scientific expertise 7. Given these benefits, its applications have been widely explored in the environment, medical and, food industries that designed either on a solid-based platform or solution-phase method 8,9. Assuredly, the integration of nanomaterials with immunosensing techniques has caused a huge improvement in its functionality and sensitivity 10-12. Nanoscale materials, with a size less than 100 nm are endowed with unique physical and chemical properties such as; high surface area, optical, and electrical characteristics, which ...

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Graphene Oxide as a Pathogen‐Revealing Agent: Sensing with a Digital‐Like Response

Cited by 14 publications

References 50 publications

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Nanopaper as an Optical Sensing Platform

Toward a nanopaper-based and solid phase immunoassay using FRET for the rapid detection of bacteria

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