Graphene oxide/gallium nanoderivative as a multifunctional modulator of osteoblastogenesis and osteoclastogenesis for the synergistic therapy of implant-related bone infection

Graphene oxide/copper nanosheet-integrated hydrogel platform as a bioactive and biocompatible scaffold to accelerate calvarial defect restoration

“…5e, f). Notably, relatively sparse green fluorescence was recorded in all the groups, followed by quantitative confirmation of ROS fluorescence intensity, indicating a significantly low level of free radicals (such as O2• -, H2O2 and • OH) accumulated in rBMSCs after coculture with the hydrogels [16,26]. As an essential trace element in human biological systems, copper plays a critical role in enzymatic homeostasis and cytokine function; however, GO or copper at high concentrations may unavoidably induce apoptosis and autophagy in mammalian cells, hampering the normal biological behaviors of rBMSCs in the coculture system [49,50].…”

“…In vitro osteogenic differentiation 2.5.1. Cell culture and osteoblast differentiation rBMSCs were used to investigate the osteogenic potential of the hydrogel scaffolds according to previous protocols [23,26]. In summary, extraction liquid from the hydrogel scaffolds was obtained after one week of degradation in α-MEM solution at 37 ℃ under sterile conditions as described previously [25,30].…”

“…In this study, real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to observe the expression levels of osteogenic-associated genes, such as ALP, Runt-related transcription Factor 2 (RUNX-2), osteocalcin (OCN) and osteopontin (OPN), and the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as the internal control [3,23,26]. The specific primer sequences used for the qPCR analysis are shown in Supplementary Table S1.…”

Research advances of nanomaterials for the acceleration of fracture healing

“…Carbon nanomaterial-based scaffolds show the advantages of being readily available and biologically and mechanically compatible. They significantly affect osteogenic differentiation, cell proliferation, and bone tissue regeneration [ 106 ]. Scaffolds serve as templates for bone stem cell development, expansion, renewal, adhesion, and differentiation, all critical in creating bone tissue.…”

Luteolin-loaded biocomposites containing tantalum and polyimide with antibacterial effects for facilitating osteogenic differentiation and bone bonding

“…Thus far, researchers have developed a variety of strategies to resist the implant infection. 15 Therefore, the endowment of implanted materials with bone-bonding and anti-bacterial capabilities via material design significantly contributes to the success of the operation, increasing the service life of the implants. Luteolin (LU) belonging to the flavonoid family is extensively found in vegetables, fruits, and herbs ( e.g.…”