Graphene‒Regulated Cardiomyogenic Differentiation Process of Mesenchymal Stem Cells by Enhancing the Expression of Extracellular Matrix Proteins and Cell Signaling Molecules

Park, Jooyeon; Park, Subeom; Ryu, Seungoh; Bhang, Suk Ho; Kim, Jangho; Yoon, Jeong‐Kee; Park, Yoon Hwan; Cho, Sung-Pyo; Lee, Seahyoung; Hong, Byung Hee; Kim, Byung Soo

doi:10.1002/adhm.201300177

Cited by 143 publications

(81 citation statements)

References 33 publications

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“…Park et al fi rstly proposed that graphene could upregulate the expressions of cardiomyogenic genes of MSCs and found that graphene promoted MSCs differentiate into the cardiomyogenic lineage without any exogenous chemical inducers. [ 156 ] The enhanced cardiomyogenic differentiation of MSCs cultured on graphene might be due to the upregulation of cell signaling molecules and gene expressions of ECM proteins. This fi nding indicated that graphene-based materials might be a novel platform for the cardiomyogenic differentiation of MSCs.…”

Section: Cardiac Regenerationmentioning

confidence: 99%

Graphene‐Based Materials in Regenerative Medicine

Ding

Liu

Fan

2015

Adv Healthcare Materials

147

100

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Graphene possesses many unique properties such as two-dimensional planar structure, super conductivity, chemical and mechanical stability, large surface area, and good biocompatibility. In the past few years, graphene-based materials have risen as a shining star on the path of researchers seeking new materials for future regenerative medicine. Herein, the recent research advances made in graphene-based materials mostly utilizing the mechanical and electrical properties of graphene are described. The most exciting findings addressing the impact of graphene-based materials on regenerative medicine are highlighted, with particular emphasis on their applications including nerve, bone, cartilage, skeletal muscle, cardiac, skin, adipose tissue regeneration, and their effects on the induced pluripotent stem cells. Future perspectives and emerging challenges are also addressed in this Review article.

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Section: Cardiac Regenerationmentioning

confidence: 99%

Graphene‐Based Materials in Regenerative Medicine

Ding

Liu

Fan

2015

Adv Healthcare Materials

147

100

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“…More recently, carbon nanomaterials such as CNT and graphene have been demonstrated to favor the attachment and cardiomyogenic differentiation of mesenchymal stem cells. [30][31][32] In addition, Notes: (A-C) Western blot showed activated β1-integrin protein expression in Bascs cultured on cNT-col scaffolds was twofold higher than those cultured on col scaffolds at day 7, while all β1-integrin remained roughly constant in these two groups. (D and E) Western blotting revealed the anti-β1-integrin antibody blocked the increase of α-actinin in the cNT-col group, while no apparent changes of α-actinin showed in Bascs grown in the control group.…”

Section: Discussionmentioning

confidence: 97%

Carbon nanotube-based substrates promote cardiogenesis in brown adipose-derived stem cells via β1-integrin-dependent TGF-β1 signaling pathway

Sun¹,

Mou²,

Li³

et al. 2016

IJN

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Stem cell-based therapy remains one of the promising approaches for cardiac repair and regeneration. However, its applications are restricted by the limited efficacy of cardiac differentiation. To address this issue, we examined whether carbon nanotubes (CNTs) would provide an instructive extracellular microenvironment to facilitate cardiogenesis in brown adipose-derived stem cells (BASCs) and to elucidate the underlying signaling pathways. In this study, we systematically investigated a series of cellular responses of BASCs due to the incorporation of CNTs into collagen (CNT-Col) substrates that promoted cell adhesion, spreading, and growth. Moreover, we found that CNT-Col substrates remarkably improved the efficiency of BASCs cardiogenesis by using fluorescence staining and quantitative real-time reverse transcription-polymerase chain reaction. Critically, CNTs in the substrates accelerated the maturation of BASCs-derived cardiomyocytes. Furthermore, the underlying mechanism for promotion of BASCs cardiac differentiation by CNTs was determined by immunostaining, quantitative real-time reverse transcription-polymerase chain reaction, and Western blotting assay. It is notable that β1-integrin-dependent TGF-β1 signaling pathway modulates the facilitative effect of CNTs in cardiac differentiation of BASCs. Therefore, it is an efficient approach to regulate cardiac differentiation of BASCs by the incorporation of CNTs into the native matrix. Importantly, our findings can not only facilitate the mechanistic understanding of molecular events initiating cardiac differentiation in stem cells, but also offer a potentially safer source for cardiac regenerative medicine.

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“…Graphene-based materials are used in the field of bone repair or organ regeneration. Both GO and rGO are utilized in osteogenic stem cells to study [28][29][30] chondrogenesis [31], adipogenesis [32], epithelial genesis [32], myogenesis, cardiomyogenesis [33,34] and neurogenesis [35].…”

Section: Introductionmentioning

confidence: 99%

Graphene and graphene oxide as nanomaterials for medicine and biology application

et al. 2018

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Graphene-and graphene oxide-based nanomaterials have gained broad interests in research because of their unique physiochemical properties. The 2D allotropic structure allows it to be used in various biological fields. The biomedical applications of graphene and its composite include its use in gene and small molecular drug delivery. It is further used for biofunctionalization of protein, in anticancer therapy, as an antimicrobial agent for bone and teeth implantation. The biocompatibility of the newly synthesized nanomaterials allows its substantial use in medicine and biology. The current review summarizes the chemical structure and biological application of graphene in various fields.

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Graphene‒Regulated Cardiomyogenic Differentiation Process of Mesenchymal Stem Cells by Enhancing the Expression of Extracellular Matrix Proteins and Cell Signaling Molecules

Cited by 143 publications

References 33 publications

Graphene‐Based Materials in Regenerative Medicine

Graphene‐Based Materials in Regenerative Medicine

Carbon nanotube-based substrates promote cardiogenesis in brown adipose-derived stem cells via β1-integrin-dependent TGF-β1 signaling pathway

Graphene and graphene oxide as nanomaterials for medicine and biology application

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Graphene‒Regulated Cardiomyogenic Differentiation Process of Mesenchymal Stem Cells by Enhancing the Expression of Extracellular Matrix Proteins and Cell Signaling Molecules

Cited by 143 publications

References 33 publications

Graphene‐Based Materials in Regenerative Medicine

Graphene‐Based Materials in Regenerative Medicine

Carbon nanotube-based substrates promote cardiogenesis in brown adipose-derived stem cells via &beta;1-integrin-dependent TGF-&beta;1 signaling pathway

Graphene and graphene oxide as nanomaterials for medicine and biology application

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Carbon nanotube-based substrates promote cardiogenesis in brown adipose-derived stem cells via β1-integrin-dependent TGF-β1 signaling pathway