Green engineering of biomolecule-coated metallic silver nanoparticles and their potential cytotoxic activity against cancer cell lines

Abstract

This report describes the synthesis of metallic silver nanoparticles (AgNPs) using extracts of four medicinal plants (Aegle marmelos (A. marmelos), Alstonia scholaris (A. scholaris), Andrographis paniculata (A. paniculata) and Centella asiatica (C. asiatica)). The bio-conjugates were characterized by UV-visible spectroscopy, scanning electron microscopyenergy dispersive spectroscopy (SEM-EDS), Fourier transform infrared spectrometry (FTIR), x-ray diffraction (XRD) and zeta potential. This analysis confirmed that UV-Vis spectral peaks at 375 nm, 380 nm, 420 nm and 380 nm are corresponding to A. marmelos, A. scholaris, A. paniculata and C. asiatica mediated AgNPs, respectively. SEM images revealed that all the obtained four AgNPs are predominantly spherical, fibres and rectangle in shape with an average size of 36-97 nm. SEM-EDS and XRD analysis confirmed the presence of elemental AgNPs in crystalline form for all the four nanoparticle samples. The phytochemicals of various medicinal plant extracts with different functional groups were responsible for reduction of Ag + to AgNPs, which act as capping and stabilizing agent. Among four types of AgNPs tested for anticancer activity, the Ap mediated AgNPs had shown enhanced activity against HepG2 cells (27.01 µg ml −1 ) and PC3 cells (32.15 µg ml −1 ).

Genotoxicity and apoptotic activity of biologically synthesized magnesium oxide nanoparticles against human lung cancer A-549 cell line

“…Acridine orange and ethidium bromide (AO/EB) dual staining can show the overall morphological change in shape and size to the treated cell when compare with non-treated cells [25,26]. The viable cell can give green colour which means the DNA is intact.…”

Comparison of biological activities of selenium and silver nanoparticles attached with bioactive phytoconstituents: green synthesized using Spermacoce hispida extract

“…Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. and selenium NPs (SeNPs) synthesized using various plant extracts are shown to exhibit various biological activities such as antimicrobial, antioxidant, anti-inflammatory, inhibition of enzymes, anti-diabetics and anti-cancer activity [4][5][6][7][8]. Hence, the need for synthesis of NPs using various other medicinal plants towards biological application is in demand.…”

<p>Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of <em>Ipomoea batatas</em> (L.) Lam</p>

“…22,26 According to the earlier studies, the cytotoxicity activity of Ib1-AgNPs and Ib2-AgNPs might be the end result of oxidative stress induced in the cells rather than the toxic effect of Ag + ions. 22,26,[73][74][75][76][77][78][79] The bio-synthesized Ib1-AgNPs and Ib2-AgNPs exhibited moderate ABTS, DPPH and NOx scavenging activities (Figure 7, Table 1). These results might arise from the interference of several functional groups existing in the Ib1 and Ib2 extracts, which might have played a vital part in the capping and stabilization procedure during Ib1-AgNPs and Ib2-AgNPs synthesis.…”