Green fabricated zinc oxide nanoformulated media enhanced callus induction and regeneration dynamics of Panicum virgatum L.

Abstract

The current study focuses on the usage of bio synthesized zinc oxide nanoparticles to increase the tissue culture efficiency of important forage grass Panicum virgatum. Zinc being a micronutrient enhanced the callogenesis and regeneration efficiency of Panicum virgatum at different concentrations. Here, we synthesized zinc oxide nanoparticles through Cymbopogon citratus leaves extract to evaluate the effect of zinc oxide nanoparticles on plant regeneration ability in switchgrass. X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) validate phase purity of green synthesize Zinc oxide nanoparticles whereas, electron microscopy (SEM) has illustrated the average size of particle 50±4 nm with hexagonal rod like shape. Energy dispersive spectroscopy X-ray (EDS) depicted major peaks of Zn (92.68%) while minor peaks refer to Oxygen (7.32%). ZnO-NPs demonstrated the incredibly promising results against callogenesis. Biosynthesized ZnO-NPs at optimum concentration showed very promising effect on plant regeneration ability. Both the explants, seeds and nodes showed dose dependent response and upon high doses exceeding 40 mg/L the results were recorded negative, whereas at 30 mg/ L both explants demonstrated 70% and 76% regeneration frequency. The results conclude that ZnO-NPs enhance the plant growth and development and tailored the nutritive properties at nano-scale. Furthermore, eco-friendly approach of ZnO-NPs synthesis is strongly believed to improve in vitro regeneration frequencies in several other monocot plants.

Biogenic ZnO Nanoparticles Synthesized from Origanum vulgare Abrogates Quorum Sensing and Biofilm Formation in Opportunistic Pathogen Chromobacterium violaceum

“…These are shown in Figure 5b and in ZnO NPs, Zn and O are present in a higher density when compared to C. The elemental analysis of the prepared ZnO NPs was carried out by the energy-dispersive X-ray spectroscopy attached with the SEM (Figure 5c). The composition of each element contained in the as-prepared material is obtained from the EDX, which gives strong peaks of 57.2% for Zinc at 1.0, 8.6 and 9.5 eV and 26.2% for oxygen at 0.5 eV (Figure 5b), which are in good agreement with the previously reported work on the synthesis of ZnO NPs [82][83][84]. The presence of the carbon peak could be because of some carbon deposition during the calcination of the ZnO sample.…”

Plant-Mediated Zinc Oxide Nanoparticles: Advances in the New Millennium towards Understanding Their Therapeutic Role in Biomedical Applications

“…Since they employed 3T3 fibroblasts from Balb mice, which are important connective tissue cells that aid in tissue repair and regeneration, and because these zinc oxide nanoparticles are benign to the cells, they can be used close to connective tissue cells. Shafique et al [ 153 ] reported that biosynthesized zinc oxide nanoparticles from a Cymbopogon citratus leaf extract were found, showing a promising callogenesis and regeneration frequency at their optimum concentrations (20 mg·L −1 and 30 mg·L −1 in the internodes and seeds, respectively) in Panicum virgatum , and it was confidently believed that this finding would open new doors in tissue culture technology by increasing the frequency of plant in vitro regeneration in different plant groupings.…”

Study of Wound-Healing Ointment Composition based on Highly Dispersed Zinc Oxide Modified with Nanoscale Silver

“…The implementation of this work is related to our development -an ointment composition -which is a combined agent with antimicrobial properties (due to the content of nanosilver) and regenerative properties (due to the content of highly dispersed Zinc oxide). Zinc oxide, being a source of the essential trace element Zinc, locally affects the skin area, releasing Zinc ions that saturate damaged tissues and promote accelerated regeneration of damaged skin [11,[22][23][24][25][26][27][28][29]. The ointment composition well moisturizes the damaged skin with the simultaneous restoration of the epidermis.…”