Green, herbicide-resistant plants by particle inflow gun-mediated gene transfer to diploid bahiagrass (Paspalum notatum)

Development of a modified transformation platform for apomixis candidate genes research in Paspalum notatum (bahiagrass)

“…We designed alternative protocols based on the information reported by Grando et al (2002) and Gondo et al (2005). A total of 2,811 mature seeds originating from Q4117 (apomictic) and 279 mature seeds from the cross Q4188 (sexual)×JS-83 (sexual) were successfully processed.…”

“…The in vitro culture and plant transformation protocols used here were adapted from previous works published by Bovo and Mroginski (1989), Grando et al (2002), Smith et al (2002), and Gondo et al (2005). Success rate in the selection of putative transformed plants is mainly dependent upon the dose rate of selective agents.…”

“…The methodology used was based on protocols reported by Grando et al (2002), Smith et al (2002), and Gondo et al (2005), with some modifications. Briefly, mature seeds originating from Q4117 (apomictic) or the cross Q4188×JS-83 (sexual) were incubated at 28°C for 48 h. Then, they were treated with concentrated H 2 SO 4 for 30 min, for dehusking and dormancy breaking.…”

“…We developed a protocol based on previous reports by Smith et al (2002), Altpeter and James (2005), and Gondo et al (2005), with modifications aimed at increasing transformation rates in our target genotypes. Sectors of 3-wk-old calli (with a 2 mm maximum diameter) originating from mature seeds of genotype Q4117 were used for bombardment.…”

“…Altpeter and Positano (2005) developed an efficient protocol for the regeneration of tetraploid bahiagrass plants from callus using mature seed explants. Smith et al (2002) obtained tetraploid transgenic plants resistant to glufosinate, and Gondo et al (2005) produced bialaphos-resistant diploid plants through callus bombardment. Altpeter and James (2005) used biolistic gene transfer into embryogenic callus derived from mature seeds to obtain paromomycin-resistant apomictic bahiagrass.…”