Green liquor pretreatment for improving enzymatic hydrolysis of corn stover

Gu, Feng; Yang, Linfeng; Jin, Yongcan; Han, Qiang; Chang, Hou‐min; Jameel, Hasan; Phillips, Robert

doi:10.1016/j.biortech.2012.08.054

Cited by 83 publications

(49 citation statements)

References 32 publications

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“…Considering the suppressed amount of by-products and 79% of the total sugar yield at the enzyme dosage of 5 FPU/g, which is comparable with the reported value of the alkali pretreatment (Gu et al 2012), the TAH140_180 sample appears to be a good candidate for a subsequent fermentation process to produce value-added chemicals from lignocellulosic biomass. Hence, the enzymatic hydrolysis with 5 FPU/g loading on TAH140_180 sample was selected for further studies on ethanol fermentation.…”

Section: Enzymatic Hydrolysis For Solid Residues After Autohydrolysissupporting

confidence: 76%

Effect of the Two-Stage Autohydrolysis of Hardwood on the Enzymatic Saccharification and Subsequent Fermentation with an Efficient Xylose-Utilizing Saccharomyces cerevisiae

et al. 2016

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To effectively utilize sugars during the fermentation process, it is important to develop a process that can minimize the generation of inhibiting compounds such as furans and acids, and a robust microorganism that can co-ferment both glucose and xylose into products. In this study, the feasibility of efficient ethanol production was investigated using a combination of two approaches: two-stage autohydrolysis of biomass and fermentation using an engineered Saccharomyces cerevisiae to produce ethanol. When the hardwood chips were autohydrolyzed at 140 °C, followed by the second treatment at 180 °C, a higher yield of sugar conversion and fewer inhibitory effects on subsequent fermentation were achieved compared with the results from single-stage autohydrolysis. A higher overall yield of ethanol resulted by using an engineered yeast strain, SR8. This observation suggests the possibility of the feasible combination of two-stage autohydrolysis and the recombinant yeast.

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Section: Enzymatic Hydrolysis For Solid Residues After Autohydrolysissupporting

confidence: 76%

Effect of the Two-Stage Autohydrolysis of Hardwood on the Enzymatic Saccharification and Subsequent Fermentation with an Efficient Xylose-Utilizing Saccharomyces cerevisiae

et al. 2016

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“…Total sugars of Masson pine, poplar, and bamboo were 62.6%, 60.1%, and 60.2%, respectively. Miscanthus had a similar amount of polysaccharides (55.7%) as rice straw ) and corn stover (Gu et al 2012). The weight percentage of the leaf in miscanthus was 57%.…”

Section: Characterization Of Raw Materialsmentioning

confidence: 76%

“…The total sugar yield of GL pretreated Masson pine was extremely low with less than 20% at 20 FPU/g-cellulose and had little change with increased TTA charge from 16% to 28%. Bamboo had a relatively low sugar yield compared with other herbaceous materials pretreated by GL, such as miscanthus, corn stover (Gu et al 2012), and rice straw (Gu et al 2013). The relative density of moso bamboo at different horizontal and vertical locations ranged from 0.553 to 1.006 g/cm 3 (Yu et al 2008) which is even higher than poplar (0.41 g/cm 3 ) (Kiaei and Samariha 2011) and Masson pine (0.449 to 0.509 g/cm 3 ) (Zhang et al 2012).…”

Section: Sed (%) =mentioning

confidence: 99%

Effects of Green Liquor Pretreatment on the Chemical Composition and Enzymatic Hydrolysis of Several Lignocellulosic Biomasses

Wang¹,

Meng²,

Min³

et al. 2014

BioResources

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Pretreatment of biomass is an extremely important step in a commercial biorefinery. For realization of lignocellulosic biomass as an alternative fuel source to occur, a fundamental understanding and critical investigation of the chosen pretreatment are essential. In this work, green liquor (GL) pretreatment of four plant species, namely Masson pine, poplar, moso bamboo, and miscanthus, was investigated to understand its effect on the chemical composition and enzymatic hydrolysis of different lignocellulosic materials. The results indicated that herbaceous materials exhibited better delignification selectivity in GL pretreatment than woody materials according to the order: miscanthus > moso bamboo > poplar > Masson pine. The effect of GL pretreatment on the enzymatic sugar yield was rather different depending on the varieties of lignocellulosic materials. Higher lignin removal with less polysaccharide degradation during GL pretreatment improved the enzymatic sugar yield.

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“…However, compared with nonwoody biomass such like corn stover (Gu et al 2012) and rice straw (Gu et al 2013), the lignin of poplar is more difficult to remove with GL pretreatment. In the severest GL pretreatment conditions, a higher ratio of hemicellulose retention and more delignification can be obtained compared with poplar pretreated with dilute acid (Tian et al 2011).…”

Section: Fig 2 Residual Lignin In Gl-pretreated Solid As a Functionmentioning

confidence: 99%

Enhancement of Enzymatic Saccharification of Poplar by Green Liquor Pretreatment

et al. 2014

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Green liquor (Na 2 S + Na 2 CO 3 , GL) pretreatment is an effective pathway for improving the enzymatic digestibility of lignocellulosic biomass for the production of bioethanol. In this work, GL was employed as a pretreatment to enhance the enzymatic saccharification of poplar. During pretreatment, the increase of H-factor and TTA charge resulted in enhanced delignification and increased degradation of polysaccharides. The sugar yield of enzymatic hydrolysis increased rapidly with increasing TTA charge in GL pretreatment, while the effect of different H-factors (from 400 to 800) on sugar yield was unnoticeable. The pretreated solid recovery was 75.5% at a lignin removal rate of 29.2% under optimized conditions of total titratable alkali (TTA) charge 20%, sulfidity 25%, and H-factor 400. The sugar yield of glucan, xylan, and total sugar of GLpretreated poplar in enzymatic hydrolysis reached up to 89.9%, 65.5%, and 82.8%, respectively, at a cellulase loading of 40 FPU/g-cellulose. Keywords INTRODUCTIONThe economic and environmental benefits that can be derived from bioethanol have led to extensive research in the bioconversion of lignocellulosic feedstocks into ethanol (Christersson 2008). Bioethanol is a renewable energy source produced through the fermentation of sugars and can reduce greenhouse gas emissions (Yang et al. 2013). Although current research and development attention have focused mainly on agricultural residues and dedicated energy crops, wood biomass (hardwood and softwood) is still an important feedstock for bioethanol production (Zhu and Pan 2010). Large quantities of wood biomass are also sustainably available in various regions of the world.The major differences between wood biomass and agricultural biomass are based on both physical properties and chemical components. Wood biomass is physically larger, structurally stronger, and denser than agricultural biomass. Its higher density significantly reduces transportation cost. Wood biomass has a higher lignin content than agricultural biomass. The lignin is one of the major hurdles for cellulase access to carbohydrate fraction in woody biomass and thus reduces the effectiveness of enzymatic hydrolysis. Therefore, more effort is required to overcome the recalcitrance of wood biomass through pretreatment for an efficient enzymatic saccharification.Poplar has been widely planted in temperate zones because of its rapid growth and ease of stock establishment through stem-or root-cuttings (Kang et al. 1996). Since the introduction of clones in the 1970s, poplar has been incorporated into many managed PEER-REVIEWED ARTICLE bioresources.com Meng et al. (2014). "Green liquor saccharification," BioResources 9(2), 3236-3247. 3237systems for the production of timber and fiber throughout south temperate central China, which has an area of roughly 600,000 km 2 (Fang et al. 1999). Poplar has many characteristics that make it suitable for plantation culture, which enables the production of large quantities of wood in short periods of time.Lignocellulosic biomas...

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Green liquor pretreatment for improving enzymatic hydrolysis of corn stover

Cited by 83 publications

References 32 publications

Effect of the Two-Stage Autohydrolysis of Hardwood on the Enzymatic Saccharification and Subsequent Fermentation with an Efficient Xylose-Utilizing Saccharomyces cerevisiae

Effect of the Two-Stage Autohydrolysis of Hardwood on the Enzymatic Saccharification and Subsequent Fermentation with an Efficient Xylose-Utilizing Saccharomyces cerevisiae

Effects of Green Liquor Pretreatment on the Chemical Composition and Enzymatic Hydrolysis of Several Lignocellulosic Biomasses

Enhancement of Enzymatic Saccharification of Poplar by Green Liquor Pretreatment

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