Green micelle and complex inclusion enhance synchronous spectrofluorimetric quantification of a novel analgesic combination: Tramadol and celecoxib in tablet dosage form and spiked human plasma

Abstract

This work offers for the first time an optimized, highly sensitive, simple, and accurate synchronized spectrofluorimetric technique for the simultaneous measurement of tramadol and celecoxib in powder form, their combined multimodal tablet, and finally spiked human plasma samples. Tramadol and celecoxib were recently released as a new drug combination to alleviate intense, sudden pain when other pain medications had failed. The technique entailed taking measurements of the fluorescence amplitudes of the synchronized spectra at Δλ = 100 nm. Excitation was made at 220 nm

First derivative synchronous spectrofluorimetric method for the simultaneous determination of tramadol and celecoxib in their dosage forms and human plasma

“…No significant increase in RFI was found, so the use of surfactants was omitted to render the method simpler (Figure 9). The reported paper documents the use of SDS surfactant in methanol at concentrations lower than its critical micelle concentration (CMC) resulting in complex formation with the desired drugs [39]. However, upon using distilled water as a solvent and using surfactants at their CMCs, no significant increase was found in the RFI of the drugs.…”

“…Table 7 summarizes the main differences between the proposed method and the reported spectrofluorimetric method [39]. Although the reported method is more sensitive regarding LOD, LOQ and linearity, the proposed method is considered greener according to eco‐scale score in addition to being more sensitive as evidenced by the SD values in plasma.…”

“…The technique has the additional advantage of narrowing the spectral bands, resulting in spectral simplification and minimization of spectral overlaps [38]. To the best of our knowledge, only one spectrofluorimetric method was reported for the concurrent estimation of tramadol and celecoxib in their co-formulated mixture and spiked human plasma [39] and two spectrophotometric methods were reported for the estimation of the aforementioned drugs in their co-formulated mixture [40,41]. The published spectrofluorimetric method [39] relies on using methanol as a solvent and sodium dodecyl sulfate as an enhancer for fluorescence intensity.…”

“…To the best of our knowledge, only one spectrofluorimetric method was reported for the concurrent estimation of tramadol and celecoxib in their co-formulated mixture and spiked human plasma [39] and two spectrophotometric methods were reported for the estimation of the aforementioned drugs in their co-formulated mixture [40,41]. The published spectrofluorimetric method [39] relies on using methanol as a solvent and sodium dodecyl sulfate as an enhancer for fluorescence intensity. The method is time consuming when applied to plasma, requiring extraction using acetonitrile followed by evaporation using rotary evaporator.…”

Sustainable Stability-Indicating spectra manipulations for the concurrent quantification of a novel Anti-COVID-19 drug and its active Metabolite: Green profile assessment

“…In recent years, there has been a change in emphasis towards analytical techniques that better conform to the requirements of eco-friendly analytical chemistry [21] .…”