Green synthesis and characterization of gold nanoparticles using extract of anti-tumor potent Crocus sativus

Metal nanoparticles (MNPs) belong mostly to the engineered type of nanoparticles and have not only unique physical and chemical properties but also different biological actions. In recent years, noble MNPs and their nano-sized agglomerates (collectively referred to as nanoparticles or particles in the subsequent sections) have been the subjects of much focused research due to their unique electronic, optical, mechanical, magnetic and chemical properties that can be significantly different from those of bulk materials. To enhance their use, it is important to understand the generation, transport, deposition, and interaction of such particles. Synthesis of MNPs is based on chemical or physical synthetic procedures and by use of biological material ("green synthesis" as an environmentally benign process) including bacteria, algae and vascular plants (mainly metallophytes). In biological methods for preparation of metal nanoparticles mainly leaf reductants occurring in leaf extracts are used. MNPs can be formed also directly in living plants by reduction of the metal ions absorbed as a soluble salt, indicating that plants are a suitable vehicle for production of MNPs. These methods used for preparation of MNPs are aimed to control their size and shape. Moreover, physicochemical properties of MNPs determine their interaction with living organisms. In general, inside the cells nanoparticles might directly provoke either alterations of membranes and other cell structures or activity of protective mechanisms. Indirect effects of MNPs depend on their physical and chemical properties and may include physical restraints, solubilization of toxic nanoparticle compounds or production of reactive oxygen species. Toxic impacts of MNPs on plants is connected with chemical toxicity based on their chemical composition (eg release of toxic metal ions) and with stress or stimuli caused by the surface, size and shape of these nanoparticles. Positive effects of MNPs were observed on the following plant features: seed germination, growth of plant seedlings, stimulation of oxygen evolution rate in chloroplasts, protection of chloroplasts from aging for long-time illumination, increase of the electron transfer and photophosphorylation, biomass accumulation, activity of Rubisco, increase of quantum yield of photosystem II, root elongation, increase of chlorophyll as well as nucleic acid level and increase in the shoot/root ratio. However, it should be stressed that MNPs impact on human and environmental health remains still unclear.

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“…HRTEM is a valuable tool to study nanoscale properties of crystalline material such as semiconductors and metals [30,31].…”

Section: Experimental Methods Applied For Characterization Of Metal Nmentioning

confidence: 99%

Metal Nanoparticles and Plants / Nanocząstki Metaliczne I Rośliny

Masarovičová¹,

Kráľová²

2013

Ecological Chemistry and Engineering S

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“…Curves a and b in figure 9 show the FT-IR spectra of bio- corresponds to the amine group stretching vibrations super imposed on the side of hydroxyl group [14]. The peak at 2920 cm -1 could be assigned to C−H stretching vibrations of methyl, methoxy and methylene groups [20].…”

Section: Fourier Transform Infrared Spectroscopy (Ft-ir) Spectroscopymentioning

confidence: 99%

“…Reduction of Au 3+ using plant extracts is advantageous since the phytochemicals have several medicinal properties which may aid in therapy and may be superior to polymer capped Au-NPs. Recently, the synthesis of Au-NPs has been reported using plants such as, Chenopodium album [10], Ocimum sanctum [11], Cassia auriculata [12], Rosa hybrida [13], Crocus sativus [14], Rosa damascena [15], Thuja orientalis [16], Terminalia chebula. [17] Ankamwar et al [18] reported the synthesis of gold nanotriangles using tamarind leaf extract and studied their potential applications in vapor sensing.…”

Section: Introductionmentioning

confidence: 99%

Mulberry leaf extract mediated synthesis of gold nanoparticles and its anti-bacterial activity against human pathogens

Adavallan

Krishnakumar

2014

Adv. Nat. Sci: Nanosci. Nanotechnol.

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Gold nanoparticles (Au-NPs) were synthesized at room temperature using Morus alba (mulberry) leaf extract as reducing and stabilizing agent. The development of plant mediated synthesis of nanoparticles is gaining importance due to its simplicity, low cost, non-toxicity, ecofriendliness, long term stability and reproducible aqueous synthesis method to obtain a selfassembly of nearly monodispersed Au-NPs. The formation and morphology of biosynthesized nanoparticles are investigated with the help of UV-Vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), atomic force microscopy (AFM), x-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR) techniques. Au-NPs formation was screened by UV-Vis spectroscopy through color conversion due to surface plasmon resonance band at 538 nm for Au-NPs. DLS studies revealed that the average size of Au-NPs was 50 nm. TEM studies showed the particles to be nearly spherical with few irregular shapes and particle size ranges 15−53 nm. The AFM image clearly shows the surface morphology of the well-dispersed Au-NPs with less than 50 nm. The high crystallinity of nanoparticles is evident from bright circular spots in the selected area electron diffraction (SAED) pattern. X-ray diffraction pattern showed high purity and face-centered cubic structure of Au-NPs. The FT-IR results indicate the presence of different functional groups present in the biomolecule capping the nanoparticles. Further, biosynthesized Au-NPs show strong zone of inhibition against Vibrio cholera (gram-negative) and Staphylococcus aureus (gram-positive) whereas, chemically synthesized Au-NPs and mulberry leaf extract exhibit a fair zone of inhibition.

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“…But synthesis of nano silver particle is economic and easy to prepared nanoparticle. Recently, plant extracts have been reported to show high efficacy in Au NPs synthesis such as Anacardium occidentale [7], Centella asiatica [8], Chenopodium album [9], Coleus amboinicus [10], Crocus sativus [11], Euphorbia hirta [12], Mangifera indica [13], Macrotyloma uniflorum [14], Trianthem adecandra [15], Murray akoenigii [16], Piper betlel [17], and seed extracts used for Terminalia chebula [18] and Cuminum cyminm [19]. Very few works had been done in the Au NPs synthesis using fruit extract such as Abelmoschus esculentus [20], Citrus limon, Citrus reticulata and Citrus sinensis [21].…”

Section: Introductionmentioning

confidence: 99%

Bactericidal and Cytotoxic Properties of Silver Nanoparticle Synthesized from Root Extract of Asparagus Racemosus

Khanra¹,

Panja²,

Choudhuri³

et al. 2016

Nano BioMed ENG

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To evaluate the bactericidal, and cytotoxicity properties of silver nanoparticles synthesized from root extract of Asparagus racemosus. The physical properties of these nanoparticles were characterized by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), XRD and UVVis spectrophotometric analysis. The biochemical properties were assayed by antibacterial study, cytotoxicity assay using cancer cell line. The formation of silver nanoparticles was confirmed by UV-VIS spectroscopic analysis which showed absorbance peak at 420 nm. X-ray diffraction photograph indicated the face centered cubic structure of the synthesized AgNPs. TEM has displayed the different dimensional images of biogenic silver nanoparticles with particle size distribution ranging from 30-50 nm. Silver particles are spherical in shape. The EDX analysis was used to identify the elemental composition of synthesized AgNPs. Antibacterial activity of the synthesized AgNPs against three Gram positive and Gram negative bacteria strains like Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa carried out showed significant zones of inhibition. The cytotoxicity study by AgNPs also showed cytotoxicity on ovarian cancer cell line PA-1. The present study confirms that the AgNPs have great promise as antibacterial, and anticancer agent.

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Green synthesis and characterization of gold nanoparticles using extract of anti-tumor potent Crocus sativus

Cited by 69 publications

References 75 publications

Metal Nanoparticles and Plants / Nanocząstki Metaliczne I Rośliny

Metal Nanoparticles and Plants / Nanocząstki Metaliczne I Rośliny

Mulberry leaf extract mediated synthesis of gold nanoparticles and its anti-bacterial activity against human pathogens

Bactericidal and Cytotoxic Properties of Silver Nanoparticle Synthesized from Root Extract of Asparagus Racemosus

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