Green Synthesis of Acid Esters from Furfural via Stobbe Condensation

Banerjee, S.; Tayade, R. A.; Sharma, Bhagyashree D.

doi:10.1155/2013/152370

Cited by 3 publications

(1 citation statement)

References 19 publications

(13 reference statements)

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“…Specifically, there was an increase in glucose and galactose levels and a decrease in xylose levels released after cell wall acid hydrolysis in both transgenic lines compared to wild-type plants. The detection of low amounts of xylose could be because in transgenic plants hemicellulose is more exposed to degradation by increasing the "easy to hydrolyze" fraction (Lavarack et al 2002), and after hydrolysis, xylose is rapidly degraded to different products, such as furfural (Banerjee et al 2013).…”

Section: Fermentable Sugar Levels Increase As a Consequence Of The Bi...mentioning

confidence: 99%

The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties

et al. 2016

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Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro. Transgenic plants overexpressing SBD123 in the cell wall are larger than wild type. Cell wall components are altered in transgenic plants. Transgenic plants are more susceptible to digestion than wild type and present higher released glucose content. Our results suggest that the transgenic plants have an advantage for the production of bioethanol in terms of saccharification of essential substrates. The plant cell wall, which represents a major source of biomass for biofuel production, is composed of cellulose, hemicelluloses, pectins and lignin. A potential biotechnological target for improving the production of biofuels is the modification of plant cell walls. This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components. In this study, we modified the cell wall of A. thaliana by targeting the starch-binding domains of A. thaliana starch synthase III to this structure. The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry. The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains. Based on these results, our approach represents a promising biotechnological tool for reducing of biomass recalcitrance and therefore, the need for pretreatments.

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Section: Fermentable Sugar Levels Increase As a Consequence Of The Bi...mentioning

confidence: 99%