Green Synthesis of Chitosan Nanoparticles Using of Martynia annua L. Ethanol Leaf Extract and Their Antibacterial Activity

Duraisamy, Narayanasamy; Dhayalan, Sangeetha; Shaik, Mohammed Rafi; Shaik, Althaf Hussain; Shaik, Jilani Purusottapatnam; Shaik, Baji

doi:10.3390/cryst12111550

Cited by 13 publications

(3 citation statements)

References 46 publications

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“…1 B). It was reported that the UV–visible spectrum of chitosan nanoparticles ranged between 200 and 322 nm as a result of the presence of the CO group 48 . The present findings are also in agreement with those of Sathiyabama and Parthasarathy 37 , who used proteins derived from Penicillium oxalicum to produce chitosan nanoparticles with a sharp peak at 285 nm.…”

Section: Resultsmentioning

confidence: 99%

Green fabrication of chitosan nanoparticles using Lavendula angustifolia, optimization, characterization and in‑vitro antibiofilm activity

El‐Naggar

Eltarahony

Hafez

et al. 2023

Sci Rep

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Chitosan nanoparticles (CNPs) are promising polymeric nanoparticles with exceptional physicochemical, antimicrobial and biological characteristics. The CNPs are preferred for a wide range of applications in the food industry, cosmetics, agriculture, medical, and pharmaceutical fields due to their biocompatibility, biodegradability, eco-friendliness, and non-toxicity. In the current study, a biologically based approach was used to biofabricate CNPs using an aqueous extract of Lavendula angustifolia leaves as a reducing agent. The TEM images show that the CNPs were spherical in shape and ranged in size from 7.24 to 9.77 nm. FTIR analysis revealed the presence of several functional groups, including C–H, C−O, CONH2, NH2, C–OH and C–O–C. The crystalline nature of CNPs is demonstrated by X-ray diffraction. The thermogravimetric analysis revealed that CNPs are thermally stable. The CNPs' surface is positively charged and has a Zeta potential of 10 mV. For optimising CNPs biofabrication, a face-centered central composite design (FCCCD) with 50 experiments was used. The artificial intelligence-based approach was used to analyse, validate, and predict CNPs biofabrication. The optimal conditions for maximum CNPs biofabrication were theoretically determined using the desirability function and experimentally verified. The optimal conditions that maximize CNPs biofabrication (10.11 mg/mL) were determined to be chitosan concentration 0.5%, leaves extract 75%, and initial pH 4.24. The antibiofilm activity of CNPs was evaluated in‑vitro. The results show that 1500 μg/mL of CNPs suppressed P. aeruginosa, S. aureus and C. albicans biofilm formation by 91.83 ± 1.71%, 55.47 ± 2.12% and 66.4 ± 1.76%; respectively. The promising results of the current study in biofilm inhibition by necrotizing biofilm architecture, reducing its significant constituents and inhibiting microbial cell proliferation encourage their use as natural biosafe and biocompatible anti-adherent coating in antibiofouling membranes, medical bandage/tissues and food packaging materials.

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Section: Resultsmentioning

confidence: 99%

Green fabrication of chitosan nanoparticles using Lavendula angustifolia, optimization, characterization and in‑vitro antibiofilm activity

El‐Naggar

Eltarahony

Hafez

et al. 2023

Sci Rep

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“…The similarity of the Rf values indicated that some of the ethyl acetate extract compounds were successfully encapsulated in the nanoparticles. Several factors affect the undetectable component with TLC including sorbent quality, humidity, plate thickness, elution distance, and ambient temperature [34]. Another factor that can exert an influence is the method of nanoparticle preparation, where some particles agglomerate and condense.…”

Section: Characterization Of Nanoparticlesmentioning

confidence: 99%

Antibacterial Activity and Characterization of Chitosan Nanoparticles Prepared from Hijau Lumut Banana ( Musa paradisiaca L.) Peel Ethyl Acetate Extract

Rita,

Yanti,

Swantara

2023

Nano Biomedicine and Engineering

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Peels of Hijau lumut banana (HLB) (Musa paradisiaca L.) are a form of organic waste that has not been widely utilized. This study was conducted to determine the antibacterial activity of ethyl acetate extract of HLB peels and that of their nanoparticles at various concentrations of chitosan against Staphylococcus aureus and Escherichia coli. It was also aimed to characterize the most active nanoparticles. Extraction of HLB peels was carried out by maceration and partitioning methods. Ionic gelation was applied to synthesize nanoparticles using chitosan crosslinked with sodium tripolyphosphate (NaTPP). Antibacterial activity assay was carried out by the agar diffusion well method. The nanoparticles were characterized by the following analyses: determination of their size distribution and zeta potential by a particle size analyzer, and of their morphology by scanning electron microscopy and transmission electron microscopy, phytochemical screening to determine secondary metabolite contents, determination of retention factor values and staining patterns by thinlayer chromatography, ultraviolet-visible (UV-Vis) spectrophotometry to confirm nanoparticle formation, and determination of the characteristics of their functional groups by Fourier transform infrared spectrophotometry. The antibacterial activity assay showed that the nanoparticles exhibited a stronger antibacterial activity than the ethyl acetate extract, with chitosan at a concentration of 1% being associated with the highest activity. In terms of their morphology, the nanoparticles were amorphous (non-uniform), while they absorbed UV-Vis at 385 nm and had the following functional groups: -OH, -NH, -CH, and P-O. This study revealed that these nanoparticles exert antibacterial effects and the secondary metabolites were encapsulated via crosslinking between chitosan and NaTPP.

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“…Research on the plants and their products or formulations used in traditional medicine has attracted a lot of interest in recent years. Indigenous cultures globally have turned to herbal remedies as a result of the resurgence of interest in medicinal plants [ 2 ]. Natural resources are becoming increasingly sought-after as a source for the creation of nutraceuticals, medications, medicines, and various cosmetics.…”

Section: Introductionmentioning

confidence: 99%

An In Vitro Evaluation of Anti-inflammatory and Antioxidant Activities of Cocos nucifera and Triticum aestivum Formulation

Pandiar,

Shanmugam

et al. 2023

Cureus

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Background Medicinal plants are traditionally used in Ayurveda, Unani medicine, and Siddha as primary sources of drugs, and mankind has exploited the therapeutic properties of these herbs throughout history. Coconut ( Cocos nucifera ), a common ingredient of Indian sub-continental cuisine, has been proven to possess various medicinal properties; similarly, wheatgrass ( Triticum aestivum ) is of greater medicinal value and is known as the powerhouse of nutrients and vitamins. These have been used individually, but there is limited data on the synergistic use of these products. Thus, the present in vitro study was designed to prepare an oral gel from the extract of C. nucifera and T. aestivum and to assess its cumulative anti-inflammatory and antioxidant activity. Materials and methods C. nucifera extract and T. aestivum extract were prepared separately, and gel formulation was done. The formulated gel was tested for its anti-inflammatory and antioxidant activity. Results The results of the present study demonstrated that the anti-inflammatory property of the gel formulation was greater as compared to the standard (diclofenac), with the highest percentage of inhibition of 90.1% at 50 μl. With regard to the antioxidant property, we found that it was comparable to the standard (ascorbic acid) at various concentrations, with greater activity at 50 μl. Conclusion The oral gel formulation of coconut ( C. nucifera ) and wheatgrass ( T. aestivum ) showed better anti-inflammatory and a comparable antioxidant activity. Thus, this formulation may be employed as an adjunct to the commercially available oral gel preparations.

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Green Synthesis of Chitosan Nanoparticles Using of Martynia annua L. Ethanol Leaf Extract and Their Antibacterial Activity

Cited by 13 publications

References 46 publications

Green fabrication of chitosan nanoparticles using Lavendula angustifolia, optimization, characterization and in‑vitro antibiofilm activity

Green fabrication of chitosan nanoparticles using Lavendula angustifolia, optimization, characterization and in‑vitro antibiofilm activity

Antibacterial Activity and Characterization of Chitosan Nanoparticles Prepared from Hijau Lumut Banana ( Musa paradisiaca L.) Peel Ethyl Acetate Extract

An In Vitro Evaluation of Anti-inflammatory and Antioxidant Activities of Cocos nucifera and Triticum aestivum Formulation

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