Green Synthesis of Copper Nano-Drug and Its Dental Application upon Periodontal Disease-Causing Microorganisms

Abstract

Dental pathogens lead to chronic diseases like periodontitis, which causes loss of teeth. Here, we examined the plausible antibacterial efficacy of copper nanoparticles (CuNPs) synthesized using Cupressus macrocarpa extract (CME) against periodontitis-causing bacteria. The antimicrobial properties of CME-CuNPs were then assessed against oral microbes (M. luteus. B. subtilis, P. aerioginosa) that cause periodontal disease and were identified using morphological/ biochemical analysis, and 16S-rRNA techniques. The CME-CuNPs were characterized, and accordingly, the peak found at 577 nm using UV-Vis spectrometer showed the formation of stable CME-CuNPs. Also, the results revealed the formation of spherical and oblong monodispersed CME-CuNPs with sizes ranged from 11.3 to 22.4 nm. The FTIR analysis suggested that the CME contains reducing agents that consequently had a role in Cu reduction and CME-CuNP formation. Furthermore, the CME-CuNPs exhibited potent antimicrobial efficacy against different isolates which was superior to the reported values in literature. The antibacterial efficacy of CME-CuNPs on oral bacteria was compared to the synergistic solution of clindamycin with CME-CuNPs. The solution exhibited a superior capacity to prevent bacterial growth. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and fractional inhibitory concentration (FIC) of CME-CuNPs with clindamycin recorded against the selected periodontal disease-causing microorganisms were observed between the range of 2.6-3.6 μg/ml, 4-5 μg/ml and 0.312-0.5, respectively. Finally, the synergistic antimicrobial efficacy exhibited by CME-CuNPs with clindamycin against the tested strains could be useful for the future development of more effective treatments to control dental diseases.

Antimicrobial Efficacy of Glass Ionomer Cement in Incorporation with Biogenic Zingiber officinale Capped Silver-Nanobiotic, Chlorhexidine Diacetate and Lyophilized Miswak

“…TEM image of the ZOE-AgNPs (Figure 2) displayed a spherical shape with average size from 10.5 to 14.12 nm. Our finding results were almost in agreement with previous findings [27].…”

Novel Antibacterial, Cytotoxic and Catalytic Activities of Silver Nanoparticles Synthesized from Acidophilic Actinobacterial SL19 with Evidence for Protein as Coating Biomolecule

“…Therefore, in our study on the biosynthesis of AgNPs we paid particular attention to such a group of microorganisms. In addition, biogenic synthesis by actinomycetes is an eco-friendly, easy, and cost-effective approach [ 22 , 24 ].…”

Comparison and Advanced Antimicrobial Strategies of Silver and Copper Nanodrug-Loaded Glass Ionomer Cement against Dental Caries Microbes

“…This analysis was conducted using a UV–Vis spectrometer (Shimadzu UV-1650, Tokyo, Japan) in the range of 300–800 nm to confirm the manufacture of TVE-CuNPs [ 15 ]. After that, the final mixture was centrifuged at 10,000× g to separate the TVE-CuNPs.…”

“…A thin layer of well-grinded dry TVE-CuNPs was distributed on the glass slide that was introduced into the XRD chamber to study the XRD pattern. The phase of TVE-CuNPs was characterized using Shimadzu XRD (3A, Tokyo, Japan), and the spectra were recorded by CuKα radiation with a wavelength of 1.5406Å in the 2θ (from the range of 20–80°) [ 15 ].…”

“…The GIC surface erodes because of biofilm formation [ 14 ]. To solve this problem, restorative materials have been mixed with antibiotics in previous studies because they have antimicrobial properties [ 15 ]. Nonetheless, due to the emergence of multidrug resistant pathogens, traditional antimicrobials are becoming ineffective in treating oral infections.…”