Green synthesis of CuO NPs, characterization and their toxicity potential against HepG2 cells

Abstract

In this study, copper oxide nanoparticles (CuO NPs) were green synthesized using the leaf extract of Momordica cochinchinensis (Lour.). Various characterization techniques such as Energy-dispersive x-ray spectroscopy (EDS), Ultraviolet Visible (UV–vis) Spectroscopy, x-ray diffraction (XRD), Transmission Electron Microscopy (TEM) and Fourier-transform Infrared (FTIR) Spectroscopy were utilized to study the formation of CuO NPs. DLS and TEM analysis revealed the formation of sphere-shaped CuO NPs with mean particle size of 56 nm. Additionally, the prepared CuO NPs were incubated with Hepatic (HepG2) cells to check their cel viability and evaluate the formation of reactive oxygen species (ROS). The results of the current work exhibited a concentration-dependent decline in the viability of HepG2 cells with half maximal inhibitory concentration (IC50) value of 75 μg ml−1. The cytotoxic effect of CuO NPs was responsible for the cell apoptosis as well as ROS induction in the HepG2 cells. Further, the prepared CuO NPs could act as possible chemotherapeutic agent for the treatment of liver hepatocellular carcinoma (HCC) in future.

Biogenic synthesis of copper oxide nanoparticles: comprehensive in vitro profiling for cervical cancer treatment and antibacterial strategies

“…), observing a zeta potential of −24 mV and a hydrodynamic diameter of 56 nm. 82 The comparisons with prior studies highlight the importance and reliability of the obtained zeta potential and hydrodynamic size values in our study, further confirming the suitability of the synthesized nanoparticles for specific applications in accordance with the objectives of this investigation.…”

Biogenic copper oxide nanoparticles from Bacillus coagulans induced reactive oxygen species generation and apoptotic and anti-metastatic activities in breast cancer cells

“…Various experiments indicated that CuONPs could induce ROS generation in different cancer cells, including HepG2 93 , SK-Hep-1 12 , K562 17 , tumor-initiating cells-enriched PANC1 15 , and MCF-7 cells 78 . In another study, the incubation of HepG2 cancer cells with biosynthesized CuONPs at concentrations of 25–100 μg/mL for 48 h resulted in the elevation of ROS generation, compared to control cells 64 .

Figure 12 ROS levels in ( A ) MCF7 and ( B ) SKBR3 cell lines treated with IC 50 concentration of BC-CuONPs after 48 h. ***:P < 0.001.

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“…Gnanavel et al’s study and Obaid et al’s investigation indicated that the synthesized CuONPs show great anticancer effects on HCT-116 and HT-29 cancer cell lines with the IC 50 value of 40 μg/mL for 24 h, which was close to the current study’s findings 62 , 63 . Moreover, an investigation showed that the cell viability of HepG2 cell lines had a significant decrease in time- and dose-depended manner after treatment with biosynthesized CuONPs using Momordica cochinchinensis 64 . Another study indicated that the IC 50 values of biosynthesized CuONPs using Annona muricata extract for AMJ-13 and MCF-7 cells were 17.04 and 18.92 µg mL −1 , respectively, which demonstrates that the uptake of CuONPs by cancer cells could trigger apoptosis 6 .…”

Biogenic Synthesis of Copper Oxide Nanoparticles Using Pimpinella anisum Seed Extract Characterization and Antibacterial activity

“…Due to the wide abundant of the medicinal plants and its costly effective, there is a considerable growth in the synthesis of copper oxide nanoparticles utilizing green chemicals. [10][11][12] Copper oxide nanoparticles have attracted interest among numerous metal nanoparticles because they exhibit mechanical and biological features using modern technologies 13 . Copper oxide nanoparticles have been discovered to be particularly effective in gas sensors, photocatalytic applications, and the biomedical sciences for the treatment of numerous disorders 14 .…”