Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents

Abstract

ObjectivesEnzyme/metal-organic framework composites with high stability in protein denaturing solvents were reported in this study.ResultsEncapsulation of enzyme in metal-organic frameworks (MOFs) via co-precipitation process was realized, and the generality of the synthesis was validated by using cytochrome c, horseradish peroxidase, and Candida antarctica lipase B as model enzymes. The stability of encapsulated enzyme was greatly increased after immobilization on MOFs. Remarkably, when exposed to protein denaturing solvents including dimethyl sulfoxide, dimethyl formamide, methanol, and ethanol, the enzyme/MOF composites still preserved almost 100% of activity. In contrast, free enzymes retained no more than 20% of their original activities at the same condition. This study shows the extraordinary protecting effect of MOF shell on increasing enzyme stability at extremely harsh conditions.ConclusionThe enzyme immobilized in MOF exhibited enhanced thermal stability and high tolerance towards protein denaturing organic solvents.Electronic supplementary materialThe online version of this article (doi:10.1186/s40643-017-0154-8) contains supplementary material, which is available to authorized users.

Rapid In Situ Encapsulation of Laccase into Metal‐Organic Framework Support (ZIF‐8) under Biocompatible Conditions

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Magnetic ZIF-8/cellulose/Fe3O4 nanocomposite: preparation, characterization, and enzyme immobilization

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Continuous Production of Ursodeoxycholic Acid by Using Two Cascade Reactors with Co‐immobilized Enzymes

“…In order to achieve continuous synthesis in a PBR for the production of UDCA, we first tried to immobilize the four desired enzymes for use in cascade reactions (Scheme ). Initially, a number of immobilization carriers or methods were tested, including gel entrapment, cross‐linking with glutaraldehyde, metal–organic frameworks (MOFs), and inorganic crystal nanoflowers; however, the activities of the resulting immobilized enzymes were very low (data not shown). Finally, we chose commercially available resins with active epoxy groups as a carrier to immobilize the enzymes.…”