Green Synthesis Of Gold Nanoparticles For controlled Delivery

Abstract

Gold nanoparticles (AuNPs) have been synthesized by green method using chitosan as a reducing/capping agent. We designed a biocompatible carrier for controlled release of hydrophobic drugs. The designed carrier was prepared by using single oil-inwater (O/W) emulsion. The resulting AuNPs were characterized by UV-Vis spectroscopy (UV-Vis) and Fourier transform infrared spectroscopy (FTIR). The transmission electron microscopy (TEM) studies indicate the spherical nature of drug loaded nanoparticles with the size of 50nm while the average particle size of AuNPs is found to be 2-3nm. The chitosan capped AuNPs showed a surface plasmon resonance at 526nm. The FTIR spectra suggest that the amine group is mainly responsible for the reduction of tetrachloroauric acid and capping the AuNPs. The controlled release of rifampicin (RIF) was investigated by in vitro studies using phosphate buffer saline (PBS) at pH=7.4. The loading efficiency of drug molecule was found to be 71%. The encapsulated drugs were released at 37 °C temperature. The results have been fit into various mechanistic models and it is found that the Higuchi model fits in to the release behavior of RIF. Further, the antibacterial activity of RIF loaded nanoparticles was examined by Gram +ve (bacillus subtils) and Gram -ve (Pseudomonas aeruginosa) bacteria. The application of similar drug loaded nanocarrier for treating other diseases like cancer can also be investigated.

Improving the performance of chitosan in the synthesis and stabilization of gold nanoparticles

“…Finally, nanoparticles obtained using chitosan-g-PCL were spherical with the larger diameter of approximately 40.4 nm. These results are coincident with the bibliography since, generally gold nanoparticles synthesised using chitosan are reported spherical in shape [45,46,54], although some triangular shaped nanoparticles have also been observed [55].…”

A review of drug delivery systems based on nanotechnology and green chemistry: green nanomedicine

“…They used single oil-in-water (O/W) emulsions for synthesizing a biocompatible controlled release of rifampicin in the body. 60 Another safe, cost-effective, and environmentally friendly method to prepare AgNPs in aqueous media was displayed using Ag nitrate (AgNO 3 ) and freshly extracted egg whites. The results exhibited that biomolecule-loaded AgNPs have mainly spherical shapes and the average sizes of 20 nm.…”

Preparation and characterization of chitosan-capped radioactive gold nanoparticles: neutron irradiation impact on structural properties

“…Chitosan is a derivative of glucose that exhibits electronegative behavior and also acts as a polyelectrolyte electrostatic stabilizer [26,27]. Due to the poor solubility of chitosan in water, it was dissolved in 1 % acetic acid solution and then the mixture was kept overnight until a clear solution was obtained.…”