Green synthesis of silver nanoparticles: Antimicrobial potential and chemosensing of a mutagenic drug nitrofurazone in real samples

Ahmed, Shah Rizwan; Anwar, Humera; Ahmed, Syed Waseem; Shah, Mohammad Raza; Ahmed, Ayaz; Ali, Syed Abid

doi:10.1016/j.measurement.2021.109489

Cited by 14 publications

(12 citation statements)

References 44 publications

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“…Ahmed et al [36] reported the green synthesis of AgNPs using dried leaf extract of Tasmanian flax-lily and evaluated their antibacterial activity against several microbes including S. aureus, S. epidermidis, P. aeruginosa and C. albicans. Chlorophytum borivilianum callus extract was utilized for the green synthesis of AgNPs and the synthesized nanoparticle was used to investigate the antimicrobial activity towards the human pathogens such as B. subtilis, S. aureus, P. aeruginosa and E. coli.…”

Section: Antibacterial Application Of Green Synthesized Agnpsmentioning

confidence: 99%

Green Synthesis and Potential Antibacterial Applications of Bioactive Silver Nanoparticles: A Review

et al. 2022

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Green synthesis of silver nanoparticles (AgNPs) using biological resources is the most facile, economical, rapid, and environmentally friendly method that mitigates the drawbacks of chemical and physical methods. Various biological resources such as plants and their different parts, bacteria, fungi, algae, etc. could be utilized for the green synthesis of bioactive AgNPs. In recent years, several green approaches for non-toxic, rapid, and facile synthesis of AgNPs using biological resources have been reported. Plant extract contains various biomolecules, including flavonoids, terpenoids, alkaloids, phenolic compounds, and vitamins that act as reducing and capping agents during the biosynthesis process. Similarly, microorganisms produce different primary and secondary metabolites that play a crucial role as reducing and capping agents during synthesis. Biosynthesized AgNPs have gained significant attention from the researchers because of their potential applications in different fields of biomedical science. The widest application of AgNPs is their bactericidal activity. Due to the emergence of multidrug-resistant microorganisms, researchers are exploring the therapeutic abilities of AgNPs as potential antibacterial agents. Already, various reports have suggested that biosynthesized AgNPs have exhibited significant antibacterial action against numerous human pathogens. Because of their small size and large surface area, AgNPs have the ability to easily penetrate bacterial cell walls, damage cell membranes, produce reactive oxygen species, and interfere with DNA replication as well as protein synthesis, and result in cell death. This paper provides an overview of the green, facile, and rapid synthesis of AgNPs using biological resources and antibacterial use of biosynthesized AgNPs, highlighting their antibacterial mechanisms.

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Section: Antibacterial Application Of Green Synthesized Agnpsmentioning

confidence: 99%

Green Synthesis and Potential Antibacterial Applications of Bioactive Silver Nanoparticles: A Review

et al. 2022

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“…In order to establish a time-dependent interaction between the drugs, samples were taken from the reaction mix at different time intervals and followed spectroscopically. For concentration dependence, the different concentrations of NTZ (i.e., 0-100 μM) were allowed to react with a fixed concentration of AZT (100 μM), and incubated at room temperature for 15 min, and analyzed spectroscopically [20,21]. Likewise, in a parallel experiment variable concentrations of AZT (i.e., 0-100 μM) were reacted with a fixed NTZ concentration (100 μM) and measured for their interaction as aforementioned.…”

Section: Drug-drug Interaction Analysismentioning

confidence: 99%

“…Likewise, the effect of temperature, pH, and ionic strength of the medium on the formation of NTZ: AZT complex was also established. Moreover, drug-drug interaction (or the formation of complex) was also validated in real samples of fresh human blood plasma and urine ideally as in the protocols described earlier by us [19,21].…”

Section: Drug-drug Interaction Analysismentioning

confidence: 99%

Nitazoxanide and azithromycin for the early treatment of COVID‐19: A multi‐spectroscopic, biochemical and computational drug–drug interaction study

Ali

Abid

Ahmed

et al. 2023

J Chinese Chemical Soc

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Herein, we present an experimental and theoretical drug-drug interaction study between nitazoxanide (NTZ) and azithromycin (AZT) in an aqueous solution. Interaction was studied by using UV/Vis, fluorescence, attenuated total reflectance-fourier transform infrared (ATR-FTIR), and circular dichroism (CD) spectroscopy, while molecular docking studies were performed to establish the interaction computationally. A bright yellow color was observed when the two drugs interacted, giving a hyperchromic band at 420 nm. The rate of absorbance was linearly increased by increasing drug concentrations and in a time-dependent manner. Stability of the interaction complex (i.e., NTZ: AZT) was measured at variable temperatures (25-80 C), pH (5.0-10.0) and ionic strength (0.05-2.0 M NaCl), and not only proved stable but also retained antimicrobial potential with reduced cellular toxicity. Mole ratio and Job's method of continuous variations were used to establish the binding stoichiometry and found to be 2:1. The calculated binding constant (k b = 8,400 M À1 ) and Gibb's free energy (ΔG = À22.4 KJ/mol) also suggested an energetically favorable interaction. FTIR spectra of NTZ: AZT complex in comparison with two drugs alone revealed significant interaction which was nicely complemented by molecular docking studies. Interaction was also successfully demonstrated in presence of carrier protein HSA and by spiking the two drugs in real samples of human plasma and urine.

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“…Likewise, the effect of temperature, pH and ionic strength of the medium on the formation of NTZ: AZT complex was also established. Moreover, drug-drug interaction (or the formation of complex) was also validated in the real samples of fresh human blood plasma and urine ideally as the protocols described earlier by us [18,21].…”

Section: Drug-drug Interaction Analysismentioning

confidence: 99%

Nitazoxanide and Azithromycin for the early Treatment of Covid-19: A Multi-Spectroscopic, Biochemical and Computational Drug-Drug Interaction Study

Ali

Abid

Ahmed

et al. 2022

Preprint

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Current investigation presented an experimental and theoretical drug-drug interaction study between nitazoxanide (NTZ) and azithromycin (AZT) in aqueous solution. Understudy drugs alone, in combination, and with other drugs are widely prescribed for the early treatment of COVID-19. Interaction was primarily studied by using UV/Vis, fluorescence, ATR-FTIR and CD spectroscopy. While molecular docking studies was performed to established the drugs interaction computationally. Two drugs when allowed to interact, the bright yellow color was observed giving hyper chromic band at 420 nm. The rate of absorbance was linearly increased with increasing drug concentrations and in a time-dependent manner. Stability of the interaction complex (i.e. NTZ: AZT) was measured at variable temperature (25–80 °C), pH (5.0–10.0) and ionic strength (0.05-2.0 M NaCl), and not only proved stable but also retain antimicrobial potential with reduced cellular toxicity. Mole ratio and Job`s method of continuous variation were used to established the binding stoichiometry between NTZ and AZT and found to be 2:1. While, the calculated binding constant (kb = 8400 M− 1) and Gibb’s free energy (ΔG° = -22.4 KJ/mol) were also suggested the energetically favorable interaction. FTIR spectra of NTZ: AZT complex in comparison with two drugs alone revealed significant interactions which was also complemented from molecular docking studies. The interaction was also successfully demonstrated in presence of carrier protein HSA and by spiking the two drugs in real samples of fresh human plasma and urine. Cumulatively, present study provided new protocol for drug’s interaction, functional efficacy and bioavailability in combination therapy.

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Green synthesis of silver nanoparticles: Antimicrobial potential and chemosensing of a mutagenic drug nitrofurazone in real samples

Cited by 14 publications

References 44 publications

Green Synthesis and Potential Antibacterial Applications of Bioactive Silver Nanoparticles: A Review

Green Synthesis and Potential Antibacterial Applications of Bioactive Silver Nanoparticles: A Review

Nitazoxanide and azithromycin for the early treatment of COVID‐19: A multi‐spectroscopic, biochemical and computational drug–drug interaction study

Nitazoxanide and Azithromycin for the early Treatment of Covid-19: A Multi-Spectroscopic, Biochemical and Computational Drug-Drug Interaction Study

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