Green synthesis of silver nanoparticles using Arnebia nobilis root extract and wound healing potential of its hydrogel

Abstract

T he present study reports wound healing potential of silver nanoparticles (AgNPs) hydrogel using Arnebia nobilis (A. nobilis) root extract. It makes a convenient method for the green synthesis of AgNPs and evaluated for its wound healing activity. Silver has been used for the treatment of medical ailments for over 100 years due to its natural antibacterial and antifungal properties. The synthesized nanoparticles were characterized using UV-visible spectrophotometer; transmission electron microscopy, X-ray diffraction (XRD), scanning electron microscopy, and Fourier transform infra-red spectrometry. The nanoparticles were found to be mostly spherical in shape. XRD study shows that the particles are crystalline in nature with face centered cubic geometry. The synthesized AgNPs exhibited good antibacterial potential against both Gram-positive and Gram-negative bacterial strain as measured using well diffusion assay. The recent emergence of nanotechnology has provided a new therapeutic modality in AgNPs for use in wounds. We investigated the wound-healing potential of AgNPs hydrogel using A. nobilis root extract in an excision animal model. The study showed that hydrogel of AgNPs using A. nobilis root extract exert positive effect due to their antimicrobial potential. The results provide insight into the mechanism of actions of AgNPs and have provided a novel therapeutic direction for wound treatment in the clinical practice.

The development of a green approach for the biosynthesis of silver and gold nanoparticles by usingPanax ginsengroot extract, and their biological applications

“…Th e synthesis of nanoparticles was monitored by the change in color of the reaction mixture. Th e reduction of silver ions was completed within 2 h of reaction at 80 ° C, and the reaction mixture completely turned from being white to being brown in color, corresponding to the surface plasmon vibrations of silver nanoparticles which resemble the synthesis of silver nanoparticles in the reaction mixture (Garg et al 2014). On the other hand, no color change was observed in a control that contained the same ratio of root extract with sterile water which was kept under similar conditions.…”

“…Th e peaks in the XRD pattern corresponding to the (111), (200), (220) and (311) planes, confi rmed the nanoparticles ' crystalline nature in the FCC structure, which was also confi rmed by bright circular spots in the SAED and clear lattice fringes in the high-resolution TEM image. Th e XRD analysis showed diff raction peaks corresponding to the FCC structure and the crystallinity of silver and gold nanoparticles (Garg et al 2014, Verma et al 2010.…”

“…For the collection and purifi cation of silver nanoparticles, the reaction mixture was fi rst centrifuged at 2000 rpm for 10 min, to remove any other unwanted components. Th en, the silver nanoparticles were obtained by centrifugation at 16,000 rpm for 20 min, and the particles were further purifi ed by repeated centrifugation followed by dispersion of the pellet in sterile water thrice, to remove the water-soluble biomolecules such as proteins and secondary metabolites (Garg et al 2014). Th is product was used for further characterization and confi rmation of silver nanoparticle synthesis.…”

“…Th e use of silver in nanoparticle form, as compared to its ionic form, appears to have reduced cellular toxicity and enhanced antibacterial effi cacy, due to the formation of free radicals. Silver nanoparticles have been found to have direct antimicrobial and anti-infl ammatory eff ects, and are mostly applied in the medical industry, to prevent infection in burns and open wounds (Dar et al 2013, Garg et al 2014). Gold nanoparticles are well known for their unique and tunable physiochemical properties and their biomedical applications (Khalil et al 2012, MubarakAli et al 2011.…”

Biosynthesis, characterization and antimicrobial studies of green synthesized silver nanoparticles from fruit extract of Syzygium alternifolium (Wt.) Walp. an endemic, endangered medicinal tree taxon

“…Hence, among the metal nanoparticles, SNPs synthesized from medicinal plants have received much attention in various biological activities like antibacterial (Savithramma et al 2011a), antifungal (Savithramma et al 2011b), anthelmintic (Seema and Amrish 2012), antilarvicidic (Sundaravadivelan et al 2013), antioxidant (Swamy et al 2014), anticancer (Vasanth et al 2014), antiinflammatory (Rafie and Hamed 2014), hepatoprotective (Bhuvaneswari et al 2014) and wound healing (Seema et al 2014) activities.…”