Green Synthesis of Silver Nanoparticles Using Randia aculeata L. Cell Culture Extracts, Characterization, and Evaluation of Antibacterial and Antiproliferative Activity

Abstract

The demand for metallic nanoparticles synthesized using green methods has increased due to their various therapeutic and clinical applications, and plant biotechnology may be a potential resource facilitating sustainable methods of AgNPs synthesis. In this study, we evaluate the capacity of extracts from Randia aculeata cell suspension culture (CSC) in the synthesis of AgNPs at different pH values, and their activity against pathogenic bacteria and cancer cells was evaluated. Using aqueous CSC extracts, AgNPs were synthesized with 10% (w/v) of fresh biomass and AgNO3 (1 mM) at a ratio of 1:1 for 24 h of incubation and constant agitation. UV-vis analysis showed a high concentration of AgNPs as the pH increased, and TEM analysis showed polydisperse nanoparticles with sizes from 10 to 90 nm. Moreover, CSC extracts produce reducing agents such as phenolic compounds (162.2 ± 27.9 mg gallic acid equivalent/100 g biomass) and flavonoids (122.07 ± 8.2 mg quercetin equivalent/100 g biomass). Notably, AgNPs had strong activity against E. coli, S. pyogenes, P. aeruginosa, S. aureus, and S. typhimurium, mainly with AgNPs at pH 6 (MIC: 1.6 to 3.9 µg/mL). AgNPs at pH 6 and 10 had a high antiproliferative effect on cancer cells (IC50 < 5.7 µg/mL). Therefore, the use of cell suspension cultures may be a sustainable option for the green synthesis of AgNPs.

Optimization of green silver nanoparticles as nanofungicides for management of rice bakanae disease

“…6 C). Similar observations have been reported previously [ 29 ]. The high R 2 value shows that the standard curve can be applied to reliably estimate the concentration of optimized AgNPs (17.24 μg/mL, maximum absorption 1.593 at 404 nm).…”

Antimicrobial and Apoptotic Efficacy of Plant-Mediated Silver Nanoparticles

“…Bands at ~3280 cm −1 represent the stretching vibration of both the hydroxyl groups of carbohydrates and N–H in amino groups of proteins [ 137 ]. The presence of proteins provides dimensional stability and allows the nanometric structure to be kept stable in the aqueous medium without the need to use surfactants to avoid agglomeration of nanoparticles [ 138 ]. A wide band within 3600–3400 cm −1 may also be attributed to the nondissociatively adsorbed water molecules, the existence of which can be confirmed by the presence of a band at ~1630 cm −1 due to the deformation vibration of the water molecules.…”

Biosynthesis of Functional Silver Nanoparticles Using Callus and Hairy Root Cultures of Aristolochia manshuriensis

“…Extracts from the callus cultures of Viola canescens were used for zinc oxide nanoparticles production [ 32 ]. The controlled conditions under which these cells are grown make them independent of environmental factors; using this technique poses no threat to biodiversity or natural resources [ 33 , 34 , 35 ]. Furthermore, cell culture enables modification of the biosynthesis of macromolecules and low molecular weight compounds responsible for ion reduction via classical approaches, media modification, elicitors, cell selection, and genetic engineering [ 36 ].…”