Green synthesis of silver nanoparticles using transgenicNicotiana tabacumcallus culture expressing silicatein gene from marine spongeLatrunculia oparinae

Abstract

In the present investigation, transgenic tobacco callus cultures and plants overexpressing the silicatein gene LoSilA1 from marine sponge Latrunculia oparinae were obtained and their bioreduction behaviour for the synthesis of silver nanoparticles (AgNPs) was studied. Synthesized nanoparticles were characterized using UV-visible spectroscopy, Fourier transformed infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), atomic flame electron microscopy (AFM) and nanoparticle tracking analysis (NTA). Our measurements showed that the reduction of silver nitrate produced spherical AgNPs with diameters in the range of 12-80 nm. The results of XRD analysis proved the crystal nature of the obtained AgNPs. FTIR analysis indicated that particles are reduced and stabilized in solution by the capping agent, which is likely to be proteins present in the callus extract. Interestingly, the reduction potential of LoSiLA1-transgenic callus line was increased three-fold compared with the empty vector-transformed calli. The synthesized AgNPs were found to exhibit strong antibacterial activity against Escherichia coli and Agrobacterium rhizogenes. The present study reports the first evidence for using genetic engineering for activation of the reduction potential of plant cells for synthesis of biocidal AgNPs.

Antimicrobial and In Vitro Cytotoxic Efficacy of Biogenic Silver Nanoparticles (Ag-NPs) Fabricated by Callus Extract of Solanum incanum L.

“…At lower Ag-NPs concentrations (100 µg mL −1 ), the values of ZOIs decreased to 16.8 ± 0.3, 13.7 ± 0.2, 17.8 ± 0.3, 17.3 ± 0.3, 15.3 ± 0.1, and 19.2 ± 0.2 mm for B. subtilis, S. aureus, P. aeruginosa, K. pneumoniae, E. coli, and C. albicans, respectively (Figure 6). Shkryl et al [16] reported that the antimicrobial activity of Ag-NPs synthesized by callus extract of Nicotiana tabacum was enhanced by increasing the concentrations of NPs. Moreover, the inhibition zones that formed due to the treatment of multi-drug resistant pathogens S. aureus and P. aeruginosa with different concentrations (25, 50, 100, and 150 µL) of Ag-NPs synthesized via Catharanthus roseus callus extract were (4.0, 7.0, 16.0, and 23.0 mm) and (5.0, 9.0, 19.0, and 29.0 mm), respectively [65].…”

“…Silver nanoparticles (Ag-NPs) have received more attention in recent decades because of their safe applications in different biomedical and biotechnological sectors [16]. Recently, Ag-NPs have been employed as antimicrobial agents, for wound healing, for anti-viral, and anti-inflammatory purposes, for the control of phytopathogenic microbes, and as anti-cancer cells and, optical receptors.…”

Biosynthesis, characterization, and in vitro assessment on cytotoxicity of actinomycete-synthesized silver nanoparticles on Allium cepa root tip cells

“…The sample for AFM was prepared by making a suspension of AgNPs with water and the addition of a droplet onto a substrate. The air-dried suspension was scanned using an AFM instrument (FLEX-AFM, Nanosurf easyscan II controller) [39]. In order to identify the biological functional groups the dried Streptomyces sp.…”

“…This was further photographed using TEM (FEI, TECNAI G2, F30) at a magnification of × 60,000 to × 80,000 [41]. Elemental analysis was done using Energy dispersive spectroscopy (EDS) coupled with SEM to identify the metal ion concentration in AgNPs [39]. Finally, X-ray diffraction (XRD) measurements of AgNPs were performed using XRD spectroscopy (Rigaku Smartlab SE).…”

Biosynthesis and Cytotoxic Properties of Ag, Au, and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract

“…The first report describing how Carica papaya callus can be used for the synthesis of silver nanoparticles (AgNPs) in the range of 60-80 nm was published by Mude and coauthors [57]. Subsequently, metal nanoparticles were obtained using extracts of Taxus yunnanensis [58], Hyptis suaveolens [59], Citrullus colocynthis [60], Panax ginseng [61], Sesuvium portulacastrum [62], Linum usitatissimum [63], Nicotiana tabacum [64], and Solanum incanum [65] cell cultures. AgNPs and gold nanoparticles (AuNPs) have been produced using Michelia champaca calli [66].…”