Green Synthesis of Zinc Oxide Nanoparticles Using Aqueous Extract of Deverra tortuosa and their Cytotoxic Activities

Selim, Yasser; Azb, Maha A.; Ragab, Islam; El‐Azim, Mohamed H. M. Abd

doi:10.1038/s41598-020-60541-1

Cited by 396 publications

(185 citation statements)

References 39 publications

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“…This variation could be attributed to a difference in the amount of ZnO NPs or changes in cell density [ 55 ]. The results obtained herein are consistent with the previous studies that reported significant cytotoxicity of ZnO NPs to human hepatocellular carcinoma HepG2 [ 56 , 57 ], bronchial epithelial BEAS-2B , lung adenocarcinoma A549 [ 58 ], colon adenocarcinoma Caco-2 [ 20 ], breast MCF-7 and MDA [ 59 ] cancer cells.…”

Section: Resultssupporting

confidence: 92%

“…This results in a change in the surface of the NPs by elevating a variety of electrons and holes, followed by the generation of ROS and oxidative stress, which eventually cause the death of cells. However, the anti-oxidative capability of the cell is surpassed; thus, ZnO NPs would act as a redox system [ 20 , 62 ].…”

Section: Resultsmentioning

confidence: 99%

“…Various studies have suggested that plant extracts are better candidates and more appropriate for large-scale green NP synthesis owing to the high rate of synthesis compared to when other organisms are used. NPs synthesized from plants have more variations in shape and size compared with those synthesized from other organisms such as bacteria, fungi and yeast [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Greener Synthesis of Zinc Oxide Nanoparticles: Characterization and Multifaceted Applications

et al. 2020

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Nanoparticles (NPs) have unique properties compared to their bulk counterparts, and they have potentials for various applications in many fields of life science. Green-synthesized NPs have garnered considerable interest due to their inherent features such as rapidity, eco-friendliness and cost-effectiveness. Zinc oxide nanoparticles (ZnO NPs) were synthesized using an aqueous extract of Kalanchoe blossfeldiana as a reducing agent. The resulting nanoparticles were characterized via X-ray diffraction (XRD), dynamic light scattering (DLS), UV-Vis spectroscopy, photoluminescence (PL), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). The antimicrobial potential of the synthesized ZnO NPs against bacterial and fungal strains was examined by the disk diffusion method, and they showed a promising antibacterial and antifungal potential. The catalytic activity of the synthesized ZnO NPs in reducing methylene blue (MB) and eosin was studied via UV-Vis spectroscopy. The decolorization percentages of the MB and Eosin Y dyes were 84% and 94%, respectively, which indicate an efficient degradation of the ZnO NPs. In addition, the cytotoxic activity of the ZnO NPs on the HeLa cell line was evaluated via in vitro assay. The MTT assay results demonstrate a potent cytotoxic effect of the ZnO NPs against the HeLa cancer cell line.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Greener Synthesis of Zinc Oxide Nanoparticles: Characterization and Multifaceted Applications

et al. 2020

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“…Also, plants produce more various nanoparticles, either in shape or in size. [1][2][3][4][5][6] The nanoparticles have broad-spectrum applications in health care, cosmetics, biomedical food and feed, drug/ gene delivery, environmental studies, mechanics, optics, light limiters, nonlinear optical and photoelectron chemical applications, and that rely on the different synthesis processes. In UV-visibleregions, the versatile semiconductor behavior, their momentous optical transparency and luminescent properties and their applications in solar cells, ceramics, and catalysts, cosmetics, gas sensors, and lighting arrestors.…”

Section: Introductionmentioning

confidence: 99%

<p>Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract</p>

AlSalhi¹,

Devanesan²,

Al-Qahtani³

et al. 2020

IJN

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To study the cytotoxic evaluation, antimicrobial and confocal analysis of zinc oxide nanoparticles (ZnO NPs) obtained from a novel plant product fennel (Foeniculum vulgare Mill.) seed extract (FSE). Methods: ZnO NPs were analyzed using UV-Vis spectroscopy, XRD, FTIR, TEM and EDX techniques. The MTT cell cytotoxicity assay measured the proliferation and survival of MCF-7 cells treated at different concentrations of FSE-derived ZnO NPs. The antimicrobial activity towards pathogenic bacteria and yeast strains was investigated. Results: The UV-Vis spectra showed two peaks at 438 nm and 446 nm, confirming nanoparticle formation. The SEM morphology results showed porous ranging from 23-51 nm. The antitumor activity value (IC 50) was at 50 µg/mL and 100 µg/mL. Besides, morphological changes of MCF-7, cells treated at different concentrations of FSE of ZnO NPs were observed in cell cultures transfected with a transient pCMV6-XL4-GFP-expressing vector containing C-terminal domain GFP-tagged proteins, which resulted in an apoptotic effect. Antimicrobial IZ ranged up No Inhibition to 18.00 ± 0.4. The IZ revealed at the highest concentration was E. faecium VRE and yeast Cryptococcus sp. (18.00 ± 0.4. mm), followed by S. aureus (17.00 ± 0.2 mm) and P. aeruginosa and the yeast C. parapsilosis (16 ± 0.4 mm). The IZ was equal to that caused by the nystatin to Cryptococcus sp., which was significantly highest than ampicillin treatments of S. aureus, P. aeruginosa, C. albicans, and C. parapsilosis. The MIC value of the FSE-derived ZnO NPs tested against E.faecium and C. albicans was 6.00 µg/mL (E. faecium and C. albicans). It was 32.00 µg/mL (S. aureus, S. typhimurium and Cryptococcus sp.), 64.00 µg/mL (P. aeruginosa), and 128 µg/mL (C. parapsilosis). Conclusion: As far as it is to our knowledge, this study established, for the first time, the biological activities of biosynthesized ZnO NPs from FSE and their synergistic therapeutic potential.

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“…Reproduced from[143] under the Creative Commons Attribution.4.1.2. Cancerous CellsNano-ZnO materials have been reported to attack multiple cancer cell lines based on cytotoxic assay results[50,126,[147][148][149][150][151][152][153][154][155][156][157][158][159][160][161][162]. Very recently, Hussain et al synthesized spherical ZnO NPs (90 nm) from Pandanus odorifer leaf extract and zinc acetate dihydrate.…”

mentioning

confidence: 99%

Interactions of Zinc Oxide Nanostructures with Mammalian Cells: Cytotoxicity and Photocatalytic Toxicity

Liao

Jin

et al. 2020

IJMS

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This article presents a state-of-the-art review and analysis of literature studies on the morphological structure, fabrication, cytotoxicity, and photocatalytic toxicity of zinc oxide nanostructures (nZnO) of mammalian cells. nZnO with different morphologies, e.g., quantum dots, nanoparticles, nanorods, and nanotetrapods are toxic to a wide variety of mammalian cell lines due to in vitro cell–material interactions. Several mechanisms responsible for in vitro cytotoxicity have been proposed. These include the penetration of nZnO into the cytoplasm, generating reactive oxygen species (ROS) that degrade mitochondrial function, induce endoplasmic reticulum stress, and damage deoxyribonucleic acid (DNA), lipid, and protein molecules. Otherwise, nZnO dissolve extracellularly into zinc ions and the subsequent diffusion of ions into the cytoplasm can create ROS. Furthermore, internalization of nZnO and localization in acidic lysosomes result in their dissolution into zinc ions, producing ROS too in cytoplasm. These ROS-mediated responses induce caspase-dependent apoptosis via the activation of B-cell lymphoma 2 (Bcl2), Bcl2-associated X protein (Bax), CCAAT/enhancer-binding protein homologous protein (chop), and phosphoprotein p53 gene expressions. In vivo studies on a mouse model reveal the adverse impacts of nZnO on internal organs through different administration routes. The administration of ZnO nanoparticles into mice via intraperitoneal instillation and intravenous injection facilitates their accumulation in target organs, such as the liver, spleen, and lung. ZnO is a semiconductor with a large bandgap showing photocatalytic behavior under ultraviolet (UV) light irradiation. As such, photogenerated electron–hole pairs react with adsorbed oxygen and water molecules to produce ROS. So, the ROS-mediated selective killing for human tumor cells is beneficial for cancer treatment in photodynamic therapy. The photoinduced effects of noble metal doped nZnO for creating ROS under UV and visible light for killing cancer cells are also addressed.

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Green Synthesis of Zinc Oxide Nanoparticles Using Aqueous Extract of Deverra tortuosa and their Cytotoxic Activities

Cited by 396 publications

References 39 publications

Greener Synthesis of Zinc Oxide Nanoparticles: Characterization and Multifaceted Applications

Greener Synthesis of Zinc Oxide Nanoparticles: Characterization and Multifaceted Applications

<p>Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract</p>

Interactions of Zinc Oxide Nanostructures with Mammalian Cells: Cytotoxicity and Photocatalytic Toxicity

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