Green Synthesis of Zinc Sulfide-Reduced Graphene Oxide Composite and its Application in Sodium-Ion Batteries

Mikhaylov, Alexey A.; Medvedev, Alexander G.; Buldashov, Ivan A.; Fazliev, Timur M.; Melnik, E. A.; Tripol’skaya, T. A.; Sladkevich, Sergey; Николаев, В. А.; Lev, Ovadia; Prikhodchenko, Petr V.

doi:10.2139/ssrn.4027265

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“…Zinc peroxide nanoparticles obtained by hydrogen peroxide sol-gel processing form homogeneous coatings on various substrates under mild conditions. [49,50] In addition, chemical and/or thermal treatment makes it possible to obtain functional materials based on zinc compounds [51]or to stabilize ZnO2 particles. [52] Thus, we propose a novel approach for the development of peroxidase-based sensor system, which is based on replacing ex-situ added H 2 O 2 with ZnO 2 nanoparticles generating in-situ H 2 O 2 on-demand by adjusting pH with buffer solution.…”

Section: Introductionmentioning

confidence: 99%

In-situ H2O2 generation-on-demand based on ZnO2 nanoparticles for optical sensor systems involving peroxidase

Kuropteva

Mikhaylov

Medvedev

et al. 2022

Preprint

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Peroxidase-based optical sensor systems are widely used in modern medicine and biosensors. As unstable hydrogen peroxide is an essential part of such sensor systems, the development of its alternative sources is an urgent and essential task leading to higher stability and reproducibility of the sensor system. Here, we propose ZnO2 nanoparticles as a source of in-situ generated H2O2 for optical sensor systems involving peroxidase and use TMB and PDA as commonly used chromogenic and fluorogenic substrates to confirm applicability of the proposed approach. The application of ZnO2 nanoparticles resulted in faster detection (up to 40% faster kinetics) and lower minimal concentration. In these experiments, Limit-Of-Detection (LOD) for peroxidase was determined as 70 units/mg under the in-situ generation of H2O2, which is 15% lower if ex-situ hydrogen peroxide was added. The proposed method for producing H2O2 on-demand is cheap, versatile, and ready-to-use in peroxidase-related biosensor applications (ELISA, DNA sensors, etc.).

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