Green Tea Catechin, EGCG, Suppresses PCB 102-Induced Proliferation in Estrogen-Sensitive Breast Cancer Cells

Abstract

The persistence of polychlorinated biphenyls (PCBs) in the environment is of considerable concern since they accumulate in human breast tissue and may stimulate the growth of estrogen-sensitive tumors. Studies have shown that EGCG from green tea can modify estrogenic activity and thus may act as a cancer chemopreventive agent. In the present study, we evaluated the individual and combined effects of PCB 102 and EGCG on cell proliferation using an estrogen-sensitive breast cancer cell line MCF-7/BOS. PCB 102 (1–10 μM) increased cell proliferation in a dose-dependent manner. Furthermore, the proliferative effects of PCB 102 were mediated by ERα and could be abrogated by the selective ERα antagonist MPP. EGCG (10–50 μM) caused a dose-dependent inhibition of PCB 102-induced cell proliferation, with nearly complete inhibition at 25 μM EGCG. The antiproliferative action of EGCG was mediated by ERβ and could be blocked by the ERβ-specific inhibitor PHTPP. In conclusion, EGCG suppressed the proliferation-stimulating activity of the environmental estrogen PCB 102 which may be helpful in the chemoprevention of breast cancer.

Natural Products for the Management and Prevention of Breast Cancer

“…Induction of apoptosis by EGCG is also exerted by the ER-independent actions with the inhibition of aryl hydrocarbon- (AhR-) regulated genes [ 103 – 106 ]. More recently, Baker and Bauer reported the antiproliferative mechanism of EGCG by blocking the ER β -specific inhibitor PHTPP [ 193 ]. In addition, EGCG is reported to induce the apoptosis in ER-negative MDA-MB-468 [ 104 ] and MDA-MB-231 cells [ 106 , 107 ] and also alters the EGFR activity [ 108 ].…”

Therapeutic Potential of Epigallocatechin Gallate Nanodelivery Systems

“…Therefore, it is urgent to investigate natural compounds like EGCG derived from green tea for the prevention and treatment of cancer and other diseases [ 59 ]. According to previous studies, EGCG is a promising molecule in the prevention and treatment of cancer [ 61 – 63 ]. Some anticancer properties of EGCG are attributed to its free radical scavenging properties, avoiding the damage of the cell structures induced by the free radicals [ 64 ].…”

Yeşil çay ve depot-medroxyprogesterone asetat infüzyonunun granüloza hücre sayısı ile ovaryan primer folikül sayı ve büyüklüğü üzerine kombine etkisi: dişi ratlarda in vivo çalışma

“…Previous findings demonstrated that EGCG has anti-proliferative effects 21,22 . Additionally, green tea also has an affinity for the ovaries and protective effects for the ovaries 10,11 .…”