Green tea extract affects porcine ovarian cell apoptosis

Influence of green tea constituents on cultured porcine luteinized granulosa cell functions

“…China) at concentrations of 0, 1, 10, and 100 μg/ml. These doses are comparable with green tea constituents level in animal and human blood after different applications of green tea extract (Wein et al, 2016;Kulandaivelu and Mandal, 2017;Law et al, 2017) and with doses used in similar previous in vitro studies (Basini et al, 2005;Wang et al, 2007;Spinaci et al, 2008;Roychoudhury et al, 2018). Substances were dissolved in culture medium immediately before their addition to the cells.…”

“…In some publications the applicability of green tea, GTPP or EGCG to increase either the bovine (Wang et al, 2007) or porcine (Spinaci et al, 2008) oocyte fertility rate and for the prevention and treatment of human and animal ovarian cell malignant transformation (Cooper, 2012;Niedzwiecki et al, 2016;Saeed et al, 2017), symptoms of polycystic ovarian syndrome (Ghafurniyan et al, 2015), and infertility (Roychoudhury et al, 2017) was demonstrated. Conversely, some authors reported no discernible effects of green tea on proliferation (the accumulation of PCNA and cyclin B1) and steroid hormones (progesterone and estradiol) in cultured porcine ovarian granulosa cells (Roychoudhury et al, 2018) or the effects of EGCG on progesterone release in cultured porcine ovarian cells or on porcine oocyte maturation (Spinaci et al, 2008), or any influence of GTPP on bovine oocyte maturation (Wang et al, 2007). Finally, in several publications the suppressive action of green tea molecules on animal reproductive processes was described.…”

“…Kao et al (2000) reported the inhibitory action of EGCG injections on murine ovarian growth and plasma levels of reproductive hormones (LH, testosterone, estradiol, leptin and insulin-like growth factor I (IGF-I)). Other authors reported the ability of green tea to promote apoptosis (the accumulation of caspase 3 and p53) (Roychoudhury et al, 2018) and the potential for EGCG treatment to suppress cultured granulosa cell proliferation, progesterone, estradiol, VEGF release, and respiration (Basini et al, 2005) and to impair oocyte fertility and embryogenesis (Spinaci et al, 2008) in pigs.…”

“…One of these key regulators is IGF-I, which can be a mediator of gonadotropin action, a suppressor of ovarian cell apoptosis, and a promoter of proliferation, which leads to ovarian follicular growth and development (Sirotkin, 2014). The publications listed below reported either no action of green tea (Roychoudhury et al, 2018) or EGCG (Spinaci et al, 2008;Roychoudhury et al, 2018) on these processes or their ability to promote apoptosis (Roychoudhury et al, 2018) and inhibit ovarian cell proliferation and steroidogenesis (Basini et al, 2005). Therefore, the available information on the action of green tea and its constituents on ovarian cells, even within a single species, is inconsistent and requires further investigation.…”

“…The variations in green tea composition can be the cause of such discrepancies; therefore, pure green tea constituents (for example, GTPP or EGCG) would be preferable for such studies. Furthermore, although the influence of green tea on FSH-induced ovarian steroidogenesis has been studied (Roychoudhury et al, 2018), no influence of green tea or its constituents on ovarian response to upstream hormonal regulators has been detected yet.…”