Green tea extract promotes DNA repair in a yeast model

Chong, Shin Yen; Chiang, Hsin-Yi; Chen, Tzu-Hung; Liang, Yi-Ju; Lo, Yi‐Chen

doi:10.1038/s41598-019-39082-9

Cited by 11 publications

(9 citation statements)

References 53 publications

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“…GTex reduces the genotoxic effects by reducing free radicals and protecting thiol groups and also has a positive effect on the DNA repair mechanism. Chong et al ( 2019 ) reported that GTex enhances expression of DNA repair genes in yeast cells.…”

Section: Resultsmentioning

confidence: 99%

Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model

Çavuşoğlu

Macar

et al. 2022

Environ Sci Pollut Res

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Mercury (Hg) is a highly toxic heavy metal for all organisms. In the present study, the mitigative role of 190 mg/L and 380 mg/L doses of green tea extract (GTex) against mercury(II) chloride (HgCI 2 )-induced toxicity was evaluated in Allium cepa L. For this aim, selected physiological, genotoxicity, and biochemical parameters as well as meristematic cell injuries in the roots were investigated. Ratios of catechin and caffeine in GTex were determined by HPLC analysis. Also, free radical scavenging activity of GTex was tested against superoxide and hydrogen peroxide radicals. As a result of HgCI 2 application, germination percentage, root elongation, weight gain, and mitotic index (MI) declined, while the frequency of micronucleus (MN), chromosomal abnormalities (CAs), and meristematic cell damages increased. HgCI 2 administration also led to a significant increase in malondialdehyde content, superoxide dismutase, and catalase activities which are signs of oxidative stress. On contrary, applications of GTex together with HgCI 2 reduced HgCI 2 -induced adverse effects in all parameters in a dose-dependent manner. Antioxidant components in GTex were listed as caffeine, epigallocatechin gallate, epigallocatechin, epicatechin gallate, and catechin according to their abundance. GTex exhibited a strong scavenging ability in the presence of superoxide and hydrogen peroxide radicals. The present study revealed the strong protective capacity of GTex against HgCI 2 -induced toxicity in A. cepa owing to its high antioxidant content with a multifaceted perspective. With this study, a reliable starting point was established for future studies investigating the more common and diverse use of GTex against toxic substances.

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Section: Resultsmentioning

confidence: 99%

Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model

Çavuşoğlu

Macar

et al. 2022

Environ Sci Pollut Res

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“…Studies across humans, animals and cell models provide evidence that polyphenols may increase the activity and expression of endogenous antioxidant enzymes, and reduce both markers of oxidative DNA damage and occurrence of strand breaks (often in response to DNA-damaging agents) [ 54 , 55 ]. Polyphenols have further been shown to increase the expression of DNA damage repair genes in yeast [ 56 ] and mice [ 57 ] under UV challenge. These studies suggest that polyphenols are biologically active when consumed, however the exact mechanism by which they may limit DNA damage in spermatozoa is unknown.…”

Section: Discussionmentioning

confidence: 99%

Mitigating the Effects of Oxidative Sperm DNA Damage

et al. 2020

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Sperm DNA damage is correlated with reduced embryo development and increased miscarriage risk, reducing successful conception. Given its links with oxidative stress, antioxidants have been investigated as a potential treatment, yet results are conflicting. Importantly, individual antioxidants are not identical in composition, and some compounds may be more effective than others. We investigated the use of the polyphenol-rich, high-antioxidant-capacity fruit acai as a treatment for elevated sperm DNA fragmentation (>16%), measured by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Following ≥ 74 days of treatment, we observed a significant decrease in sperm DNA fragmentation (−17.0% ± 2.5%) to 11.9 ± 1.7% (0–37%), with a 68.6% success rate (defined as post-treatment TUNEL < 16%). Post-treatment decreases in DNA fragmentation and success rates were not significantly impacted by low motility and/or concentration, or exceptionally high (> 25%) TUNEL. Treatment significantly reduced concentration in men with normal semen parameters, but 88% remained normal. Overall, successful treatment was not associated with age, semen parameters or TUNEL result at baseline. However, body mass index was significantly higher in nonresponders at baseline. This study provides evidence of a low-cost, effective treatment for elevated sperm DNA damage using acai.

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“…Since the issue of the dry hopping beer's quality is considered, it is necessary to consider favorable conditions for the extraction of hop polyphenol complex compounds in the presence of polar solvent (ethanol) and yeast cells, taking into account the metabolic transformations of different polyphenols [45][46][47][48], the data are presented in Table 3.…”

Section: The Determination and Mathimatical Analysis Of The Beer's Phenolic Compounds Compositionmentioning

confidence: 99%

The Influence of Hop Phenolic Compounds on Dry Hopping Beer Quality

et al. 2022

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Background: The article considers the phenolic hop compounds’ effect on the quality indicators of finished beer. The topic under consideration is relevant since it touches on the beer matrix colloidal stability when compounds with potential destabilizing activity are introduced into it from the outside. Methods: The industrial beer samples’ quality was assessed by industry-accepted methods and using instrumental analysis methods (high-performance liquid chromatography methods—HPLC). The obtained statistical data were processed by the Statistics program (Microsoft Corporation, Redmond, WA, USA, 2006). Results: The study made it possible to make assumptions about the functional dependence of the iso-α-bitter resins and isoxanthohumol content in beer samples. Mathematical analysis indicate interactions between protein molecules and different malted grain and hop compounds are involved in beer structure, in contrast to dry hopped beer, where iso-a-bitter resins, protein, and coloring compounds were significant, with a lower coefficient of determination. The main role of rutin in the descriptor hop bitterness has been established in kettle beer hopping technology, and catechin in dry beer hopping technology, respectively. The important role of soluble nitrogen and β-glucan dextrins in the perception of sensory descriptors of various technologies’ beers, as well as phenolic compounds in relation to the formation of bitterness and astringency of beer of classical technology and cold hopping, has been shown. Conclusions: The obtained mathematical relationships allow predicting the resulting beer quality and also make it possible to create the desired flavor profiles.

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Green tea extract promotes DNA repair in a yeast model

Cited by 11 publications

References 53 publications

Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model

Mitigative effect of green tea extract against mercury(II) chloride toxicity in Allium cepa L. model

Mitigating the Effects of Oxidative Sperm DNA Damage

The Influence of Hop Phenolic Compounds on Dry Hopping Beer Quality

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