Green tea polyphenol epigallocatechin-3-gallate inhibits the IL-1β-induced activity and expression of cyclooxygenase-2 and nitric oxide synthase-2 in human chondrocytes

Ahmed, Salahuddin; Rаhmаn, Аziz Ur; Hasnain, Absar-ul; Lalonde, Matthew S.; Goldberg, Victor M.; Haqqi, Tariq M.

doi:10.1016/s0891-5849(02)01004-3

Cited by 259 publications

(180 citation statements)

References 36 publications

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“…Previous studies have shown that EGCG decreased the expression of COX-2 [23] and mPGES [15] . However, the exact mechanism of the inhibi- Figure 6).…”

Section: Discussionmentioning

confidence: 86%

Prostanoid EP1 receptor as the target of (−)-epigallocatechin-3-gallate in suppressing hepatocellular carcinoma cells in vitro

et al. 2012

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Aim: To investigate the effects of (-)-epigallocatechin-3-gallate (EGCG), an active compound in green tea, on prostaglandin E 2 (PGE 2 )-induced proliferation and migration, and the expression of prostanoid EP 1 receptors in hepatocellular carcinoma (HCC) cells. Methods: HCC cell line HepG2, human hepatoma cell lines MHCC-97L, MHCC-97H and human hepatocyte cell line L02 were used. Cell viability was analyzed using MTT assay. PGE 2 production was determined with immunoassay. Wound healing assay and transwell filter assay were employed to assess the extent of HCC cell migration. The expression of EP 1 receptor and Gq protein were examined using Western blot assay. Results: PGE 2 (4-40000 nmol/L) or the EP 1 receptor agonist ONO-DI-004 (400-4000 nmol/L) increased the viability and migration of HepG2 cells in concentration-dependent manners. EGCG (100 μg/mL) significantly inhibited the viability and migration of HepG2 cells induced by PGE 2 or ONO-DI-004. HepG2 cells secreted an abundant amount of PGE 2 into the medium, and EGCG (100 μg/mL) significantly inhibited the PGE 2 production and EP 1 receptor expression in HepG2 cells. EGCG (100 μg/mL) also inhibited the viability of MHCC-97L cells, but not that of MHCC-97H cells. Both EGCG (100 μg/mL) and EP 1 receptor antagonist ONO-8711 inhibited PGE 2 4 μmol/L and ONO-DI-004 400 nmol/L-induced growth and migration of HepG2 cells. Both EGCG (100 μg/mL) and ONO-8711 210 nmol/L inhibited PGE 2 -and ONO-DI-004-induced EP 1 expression. EGCG and ONO-8711 had synergistic effects in inhibiting EP 1 receptor expression. PGE 2 , ONO-DI-004, ONO-8711, and EGCG had no effects on Gq expression in HepG2 cells, respectively. Conclusion: These findings suggest that the anti-HCC effects of EGCG might be mediated, at least partially, through the suppressing EP 1 receptor expression and PGE 2 production.

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“…Previous studies have shown that EGCG decreased the expression of COX-2 [23] and mPGES [15] . However, the exact mechanism of the inhibi- Figure 6).…”

Section: Discussionmentioning

confidence: 86%

Prostanoid EP1 receptor as the target of (−)-epigallocatechin-3-gallate in suppressing hepatocellular carcinoma cells in vitro

et al. 2012

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“…In some experiments, HaCaT cells were treated with IFN-␥ (250 -1000 IU; R&D Systems, Minneapolis, MN). Culture conditions as previously described for SF (17), thymic epithelial cells and thymic fibroblasts (kind gift of D. Patel, Thurston Arthritis Center, University of North Carolina, Chapel Hill, NC) (8), and chondrocytes (22) were maintained during these studies.…”

Section: Cell Culturementioning

confidence: 99%

Expression and Characterization of a Novel CD6 Ligand in Cells Derived from Joint and Epithelial Tissues

Saifullah

Fox

Sarkar

et al. 2004

The Journal of Immunology

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CD6 is a T cell surface glycoprotein that plays an important role in interactions of thymocytes with thymic epithelial cells and in mature T cell interactions with selected nonprofessional tissue APCs. We describe a novel CD6 ligand (CD6L) 3A11 Ag that is distinct from the known CD6L (CD166). The 3A11 protein is expressed on cells derived from human thymus, skin, synovium, and cartilage, and its expression is enhanced by IFN-γ. mAbs directed against the 3A11 Ag and CD166 exhibit distinct patterns of binding to a panel of cell lines. Confocal microscopy shows that both CD166 and the 3A11 Ag are expressed at the cell surface, and that these proteins colocalize. The 3A11 Ag has a molecular mass of 130 kDa and is immunoprecipitated using either mAb 3A11 or soluble CD6-Ig fusion protein. mAbs directed against individual CD6L were less potent than was soluble CD6-Ig fusion protein in reducing adhesion of T cells to adherent 3A11-positive epithelial cells in vitro, suggesting that these Abs recognize epitopes on the 3A11 Ag and CD166 that are distinct from CD6 binding sites. Finally, transfection of epithelial cells with CD166-specific small interfering RNAs significantly decreased CD166 expression without alteration in 3A11 Ag levels, and thus confirmed that these two CD6L are distinct. Taken together, our data identifies a novel 130-kDa CD6L that may mediate interactions of synovial and epithelial cells with T lymphocytes.

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“…epicatechin, epigallocatechin, epicatechingallate and epigallocatechingallate (9)(10)(11) . Epigallocatechingallate is the catechin most associated with the preventive effects of tea on CVD, due to its strong antioxidative properties (9,10) . In contrast to green and black tea, Rooibos tea contains dihydrochalcones, flavones and flavonols (12) but does not contain any catechins (11) .…”

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confidence: 99%

Effects of green tea, black tea and Rooibos tea on angiotensin-converting enzyme and nitric oxide in healthy volunteers

Persson

Hägg

et al. 2010

Public Health Nutr.

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Objective: Tea has been reported to reduce cardiovascular mortality, but the underlying mechanisms are largely unknown. The aim of the current project was to investigate the effect of green tea (Japanese Sencha), black tea (Indian Assam B.O.P.) and Rooibos tea (South Africa) on angiotensin-converting enzyme (ACE) and nitric oxide (NO). Design: Seventeen healthy volunteers received a single oral dose of 400 ml green tea, black tea or Rooibos tea in a randomized, three-phase, crossover study. ACE activity and NO concentration were measured (at 0, 30, 60 and 180 min) in all phases. ACE activity was analysed by means of a commercial radioenzymatic assay. Nitrite was analysed as a marker of NO concentration. In addition, ACE genotype was determined using a PCR method. Results: Oral intake of a single dose of Rooibos tea significantly inhibited ACE activity after 30 min (P , 0?01) and after 60 min (P , 0?05). A significant inhibition of ACE activity was seen with green tea for the ACE II genotype 30 min after intake of the tea (P , 0?05) and for the ACE ID genotype 60 min after intake (P , 0?05). A significant inhibition of ACE activity was also seen with Rooibos tea for the ACE II genotype 60 min after intake (P , 0?05). No significant effect on NO concentration was seen. Conclusions: These results suggest that green tea and Rooibos tea may have cardiovascular effects through inhibition of ACE activity.

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Green tea polyphenol epigallocatechin-3-gallate inhibits the IL-1β-induced activity and expression of cyclooxygenase-2 and nitric oxide synthase-2 in human chondrocytes

Cited by 259 publications

References 36 publications

Prostanoid EP1 receptor as the target of (−)-epigallocatechin-3-gallate in suppressing hepatocellular carcinoma cells in vitro

Prostanoid EP1 receptor as the target of (−)-epigallocatechin-3-gallate in suppressing hepatocellular carcinoma cells in vitro

Expression and Characterization of a Novel CD6 Ligand in Cells Derived from Joint and Epithelial Tissues

Effects of green tea, black tea and Rooibos tea on angiotensin-converting enzyme and nitric oxide in healthy volunteers

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