Greenhouse evaluation confirms in vitro sharka resistance of genetically engineered h-UTR/P1 plum plants

García-Almodóvar, R.C.; Clemente‐Moreno, María José; Díaz‐Vivancos, Pedro; Petri, César; Rubio, M.; Padilla, Isabel; Ilardi, Vincenza; Burgos, L.

doi:10.1007/s11240-014-0629-7

Cited by 20 publications

(33 citation statements)

References 21 publications

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“…The insect spent 83% of time on yellow elongated false fruits (trial 1) and 75% on yellow rounded false fruits (trial 2). In both trials, percentage of time spent on red and green false fruits were not significantly different (1-way ANOVA: trial 1, elongated fruits: F (2,9) = 10.50, p = 0.004; trial 2, rounded fruits: F (2,12) = 10.21, p = 0.003, Figure 3). In the third trial, the false fruits were different both in shape and colour, and-as it was possible to see at first glance-the time percentage spent on elongated or rounded green false fruits are all quite near to zero.…”

Section: Laboratory Trialsmentioning

confidence: 93%

“…Due to the economic and social importance of plum production, several studies evaluated new varieties adapted to regional climatic conditions, displaying resistance or tolerance to abiotic and biotic stresses (among them diseases and pests), improved fruit quantity, and overall quality [1,3,[9][10][11][12][13]. Nevertheless, a detailed examination on the discrimination of cultivars is lacking, as prior studies have been often carried out at regional level or regarded specific quality parameters [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Do Grapholita funebrana Infestation Rely on Specific Plum Fruit Features?

Rizzo

Farina

Saiano

et al. 2019

Insects

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The effective control of the plum fruit moth, Grapholita funebrana (Lepidoptera: Tortricidae) still represents a difficult challenge for organic plum farming. Little information is available on the susceptibility of plum cultivars to this moth pest. We investigated the roles of several fruit parameters (i.e., shape, volume, hardness, fruit colour, and physiochemical properties) on the susceptibility of four different plum cultivars (Angeleno, Friar, President and Stanley) to G. funebrana attack. Field data demonstrated the importance of some fruit parameters (i.e., elongation index, sugar degree, titratable acidity, cover colour percentage) on susceptibility to G. funebrana infestation. Under laboratory conditions, colour and shape had a significant role in determining the time spent on false fruits, i.e., female moths preferred yellow and rounded fruits over elongated red or green fruits. Angeleno (yellow and rounded fruits) and Stanley (green and elongated fruits) were the most and least susceptible cultivars, respectively. Overall, this study adds useful knowledge about plum cultivar susceptibility to G. funebrana. Information reported here may be useful to improve integrated pest management strategies both in conventional and organic orchards because the use of less susceptible cultivars may reduce insecticidal treatments, limiting the development of resistance in target insects and the harmful side effects on beneficial species.Insects 2019, 10, 444 2 of 16 of the susceptibility of different plum cultivars to the main pests can provide useful information for plum improvement programs and pest management strategies.Grapholita funebrana Treitschke (Lepidoptera Tortricidae), the plum fruit moth (PFM), is one of the main pests of plum orchards in Europe. This species is oligophagous and attacks different host plants within the family Rosaceae, such as the fruits of plums, cherries, and peaches. This moth can complete one or two generations per year in many parts of central and eastern Europe [19] and three generations per year in Italy [20,21]. As with other moths, females of PFM attract mates using pheromone signals [22][23][24] and lay eggs on developing fruits of host plants [25]. Neonate larvae typically bore into immature fruits, where they feed and develop until pupation. Damage is due to the feeding activity of the larvae inside the fruits, which causes changes in fruit colouration, early ripening and fruit fall. Furthermore, infested fruits show penetration holes made by neonate larvae, which are characterised by the presence of gum, and exit holes made by mature larvae leaving the fruit [26]. Both result in reduced marketability and significant yield decreases. In conventional agriculture, this pest is primarily controlled by the use of broad-spectrum insecticides [19]. In organic plum orchards, good fruit protection can be achieved by alternating a low number of spinosad applications and mineral oil applications, despite the short period of effectiveness typical of spinosad [27].In other tortricid spec...

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Section: Laboratory Trialsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Do Grapholita funebrana Infestation Rely on Specific Plum Fruit Features?

Rizzo

Farina

Saiano

et al. 2019

Insects

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“…The most efficient strategies to obtain transgenic resistance against Agrobacterium are likely those using sense and antisense sequences of ipt and iaaM split by an intron, producing hairpin RNA (hpRNA) transcripts. The expression of transgenes encoding hpRNA has been shown to be a highly effective method for the targeted silencing of specific pathogen genes, producing plants resistant to viruses that affect several economically important crops such as barley, bean, rice and tomato and some woody plants such as Mexican lime and plum …”

Section: Introductionmentioning

confidence: 99%

“…The expression of transgenes encoding hpRNA has been shown to be a highly effective method for the targeted silencing of specific pathogen genes, producing plants resistant to viruses that affect several economically important crops such as barley, 19 bean, 20 rice 21 and tomato 22 and some woody plants such as Mexican lime 23 and plum. 24,25 Transformation with self-complementary sequences of the oncogenes ipt and iaaM has been successfully used in Arabidopsis and tomato 26 and in embryogenic lines from the seed cotyledons of 'Chandler' walnuts, 27 producing crown-gall-resistant plants. These authors created a large-size T-DNA cloning most of the ipt and iaaM complete sequences in sense and antisense orientations.…”

Section: Introductionmentioning

confidence: 99%

Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot

Alburquerque

Faize

Burgos

2017

Pest Management Science

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“…Since the first development of HoneySweet plum [6][7][8], the genetic engineering approach took a great importance in our research. Use of plant or virus gene constructs are expanding [47,48], and ongoing research is producing new PPV resistant transgenic Prunus cultivars [49].…”

Section: Discussionmentioning

confidence: 99%

Innovative RNAi Strategies and Tactics to Tackle Plum Pox Virus (PPV) Genome in Prunus domestica-Plum

Ravelonandro

Scorza

Briard

2019

Plants

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We developed an innovative RNAi concept based on two gene constructs built from the capsid gene (CP) cistron of the Plum pox virus (PPV) genome. First, designated as amiCPRNA, a potential molecule interfering with PPV genome translation and the second one is the ami-siCPRNA to target viral genome translation and PPV RNA replication. Following the previous engineering of these constructs in an experimental herbaceous host, they were introduced into Prunus domestica (plum tree) genome. Previously propagated onto a susceptible rootstock, these clones were graft-inoculated with PPV. After four dormancy cycles, and consistent with our experience of PPV infection, some clones showed a common phenomenon of silencing that can differ between the detailed plant phenotypes. Three different phenotypes were developed by the amisiCPRNA clones. First, the high resistance character shown by the amisiCPRNA plum-7 that was similar to the resistance expressed by HoneySweet plum. Secondly, a recovery reaction was developed by the two other amisiCPRNA plum-3 and plum-4 that differed from the rest, characterized as susceptible clones, among these were the amiCPRNA plums. Having assessed the behavior of these plums versus the herbaceous host accumulating the similar form of RNAi: ami-, si-, and ami-siRNA, challenging assays in perennials consistently reflect the natural context of viral genome targeting.

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Greenhouse evaluation confirms in vitro sharka resistance of genetically engineered h-UTR/P1 plum plants

Cited by 20 publications

References 21 publications

Do Grapholita funebrana Infestation Rely on Specific Plum Fruit Features?

Do Grapholita funebrana Infestation Rely on Specific Plum Fruit Features?

Silencing of Agrobacterium tumefaciens oncogenes ipt and iaaM induces resistance to crown gall disease in plum but not in apricot

Innovative RNAi Strategies and Tactics to Tackle Plum Pox Virus (PPV) Genome in Prunus domestica-Plum

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