Griseofulvin-induced aneuploidy and meiotic delay in female mouse germ cells. I. Cytogenetic analysis of metaphase II oocytes

Tiveron, Cecilia; Marchetti, Francesco; Bassani, B.; Pacchierotti, Francesca

doi:10.1016/0027-5107(92)90181-z

Cited by 43 publications

(18 citation statements)

References 20 publications

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“…In fact, ovulation took place between 12 and 16 h after hCG, over 34 eggs per females were ovulated and their chromosomal constitution was normal. This compares well with the timing of ovulation in B6C3F1 females, which is known to occur between 11 and 14 h (Tiveron et al 1992). Also, data from unrelated experiments that were taking place in our laboratory at the same time as the experiments with T-stock females indicated that by 16 h after hCG an average of 36 eggs were collected from superovulated B6C3F1 females (results not shown).…”

Section: Discussionsupporting

confidence: 75%

Impaired fertility in T-stock female mice after superovulation

Zudová¹,

Wyrobek²,

Bishop³

et al. 2004

Reproduction

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Superovulation of female mice with exogenous gonadotrophins is routinely used for increasing the number of eggs ovulated by each female in reproductive and developmental studies. We report an unusual effect of superovulation on fertilization in mice. In vivo matings of superovulated T-stock females with B6C3F1 males resulted in a two-fold reduction (P < 0.001) in the frequencies of fertilized eggs compared with control B6C3F1 matings. In addition, approximately 22 h after mating, only 15% of fertilized eggs recovered in T-stock females had reached the metaphase stage of the first cleavage division versus 87% in B6C3F1 females (P < 0.0001). Matings with T-stock males did not improve the reproductive performance of T-stock females. To investigate the possible cause(s) for the impaired fertilization and zygotic development, the experiments were repeated using in vitro fertilization. Under these conditions, the frequencies of fertilized eggs were not different in superovulated T-stock and B6C3F1 females (51.7 6 6.0 and 64.5 6 3.8%, P 5 0.10). There was a seven-fold increase in the frequencies of fertilized eggs that completed the first cell cycle of development after in vitro versus in vivo fertilization in T-stock females. These results rule out an intrinsic deficiency of the T-stock oocyte as the main reason for the impaired fertility after in vivo matings, and suggest that superovulation of T-stock females may induce a hostile oviductal and uterine environment with dramatic effects on fertilization and zygotic development.

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Section: Discussionsupporting

confidence: 75%

Impaired fertility in T-stock female mice after superovulation

Zudová¹,

Wyrobek²,

Bishop³

et al. 2004

Reproduction

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“…Their reports indicated that GF was shown to induce aneuploidy and polyploidy during the first or second meiotic division in a dose-related manner, meiotic delay as measured by ovulated Met I oocytes Tiveron et al, 1992;Marchetti et al, 1992;1996;Mailhes et al, 1993 . It was also reported that the timing of chemical treatment influenced the frequencies of these effects Marchetti and Mailhes, 1994;1995 . In the present study, the effects of GF on numerical chromosome change in maturing mouse oocytes in vitro were shown From the molecular analysis of the extra chromosome in the case of human trisomies, most errors in chromosome segregation were reported to arise in the first meiotic division of oogenesis Eichenlaub-Ritter, 1996 .…”

Section: Resultsmentioning

confidence: 99%

Aneugenic effects of carbendazim and griseofulvin as assayed in the in vitro maturation system of mouse oocytes

Tanaka

Sasanami

Toriyama

et al. 2004

Environ. Mutagen Res.

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To evaluate the aneugenic effects of carbendazim MBC and griseofulvin GF on meiosis, we cultured mouse oocytes, allowing them to mature in vitro in the presence of MBC and GF. The incidence of hyperploidy was significantly increased in oocytes cultured with 0.6 µg/mL or higher concentration of MBC, while the incidence of diploidy was significantly increased in oocytes cultured with 1 or 3 µg/mL of GF. To investigate the stage-specific effects of these chemicals during meiotic progression, the oocytes were exposed to chemicals for the initial 7 h or final 8 h of the culture. Both MBC and GF were found to effectively induce numerical aberration during the final half of the culture. Thus, in vitro maturation of mouse oocytes is a useful model for the detection of chemically induced aneuploidy and for the detection of stage-specific effects of chemicals during meiosis.

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“…Morimoto et al [24] reported that gentisin and isogentisin were mutagenic for the TA100 strain of S. typhimurium. Griseofulvin induced the polyploidy in male germ cells [25] and polyploidy in human peripheral lymphocytes when administered orally [26]. Mavournin et al [10] reported that adriamycin, anthramycin, bleomycin, 4-epiadriamycin, griseofulvin, rachelmycin, sibiromycin, and tomamycin induced micronuclei in rat bone marrow cells.…”

Section: Discussionmentioning

confidence: 99%

No significant increase in chromosome aberrations and sister chromatid exchanges in cultured human lymphocytes treated with spiramycin

Rencüzoğulları¹,

İla²,

Topaktaş³

et al. 2001

Teratog Carcinog Mutagen

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In this study, the chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) were investigated in human lymphocytes treated with spiramycin antibiotic (trade name, rovamycin). Spiramycin did not induce the CAs and SCEs, and also did not decrease the mitotic index (MI). However, spiramycin decreased the replication index (RI) only at 48 h treatment times. Teratogenesis Carcinog. Mutagen. 22:51-58, 2002.

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Griseofulvin-induced aneuploidy and meiotic delay in female mouse germ cells. I. Cytogenetic analysis of metaphase II oocytes

Cited by 43 publications

References 20 publications

Impaired fertility in T-stock female mice after superovulation

Impaired fertility in T-stock female mice after superovulation

Aneugenic effects of carbendazim and griseofulvin as assayed in the in vitro maturation system of mouse oocytes

No significant increase in chromosome aberrations and sister chromatid exchanges in cultured human lymphocytes treated with spiramycin

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