Gross anatomy of the musculature and a new description of the reproductive system of Tanaisia bragai and Tanaisia inopina (Trematoda: Eucotylidae) analysed by confocal laser scanning microscopy

Abstract

D'ávila, S., Manso, P. P. A., Bessa, E. C. A., Rodrigues, M. L. A. and Dias, R. J. P. 2010. Gross anatomy of the musculature and a new description of the reproductive system of Tanaisia bragai and Tanaisia inopina (Trematoda: Eucotylidae) analysed by confocal laser scanning microscopy. -Acta Zoologica (Stockholm) 91: 139-149Confocal scanning laser microscopy has become an important tool to clarify the organization of the musculature and innervation, as well as the morphology of the reproductive and alimentary tract of various helminth species. The goal of this work was to describe the morphology of the reproductive system and the gross anatomy of the musculature of adults of the species Tanaisia bragai and Tanaisia inopina by means of confocal scanning laser microscopy. The helminths were found parasitizing the kidney collection ducts of Columba livia, in the municipality of Juiz de Fora, Brazil. These helminths were stained with Mayer's carmalum, mounted on permanent slides and observed through a confocal scanning laser microscope. The tomographic images showed the morphology of the organs and glands of the reproductive system, along with the general morphology of the musculature of the body wall, oral sucker, acetabulum, pharynx, intestinal caeca, oesophagus and reproductive system ducts. The present work is the first re-characterization of the reproductive tract of T. bragai and T. inopina. We also present the first description of the general morphology of the gametes and cells of the glands associated with the reproductive apparatus of these species.

A Simple and Rapid Staining Technique for Sex Determination of Trichinella Larvae Parasites by Confocal Laser Scanning Microscopy

“…Additionally, this staining technique is simple and rapid in comparison with known protocols, which are time consuming and consist of several steps of sample preparation. Staining of one parasite by utilizing available protocols can take more than two days and the procedure is appropriate for staining of parasite-specific tissues (Stewart et al, 2003; Davila et al, 2010; Krupenko, 2014).…”

New insights on the morphology of a digenean parasite (Digenea: Brachylaimidae,Brachylaima mazzantii(Travassos, 1927)) using confocal laser scanning microscopy

“…Some results obtained through confocal microscopy studies have confirmed evolutionary relationships. As an example, several descriptions of the muscle system (Mair et al 1998b(Mair et al , 2000Kumar et al 2003;Šebelova et al 2004;Terenina et al 2006;D'Ávila et al 2010) have strengthened the idea that the organization of the somatic musculature is conserved among the digeneans, as stated by Šebelova et al (2004). The pattern of vitellogenesis seems to be even more conserved among digeneans, monogeneans, cestodes and free-living neophoran (Swiderski & Xylander 2000;D'Ávila et al 2010).…”

“…As an example, several descriptions of the muscle system (Mair et al 1998b(Mair et al , 2000Kumar et al 2003;Šebelova et al 2004;Terenina et al 2006;D'Ávila et al 2010) have strengthened the idea that the organization of the somatic musculature is conserved among the digeneans, as stated by Šebelova et al (2004). The pattern of vitellogenesis seems to be even more conserved among digeneans, monogeneans, cestodes and free-living neophoran (Swiderski & Xylander 2000;D'Ávila et al 2010). In addi-tion, the morphological characterization of the musculature of tubular organs and attachment apparatus has supported some functional interpretations (Mair et al 1998b(Mair et al , 2000Šebelova et al 2004;D'Ávila et al 2010).…”

“…The pattern of vitellogenesis seems to be even more conserved among digeneans, monogeneans, cestodes and free-living neophoran (Swiderski & Xylander 2000;D'Ávila et al 2010). In addi-tion, the morphological characterization of the musculature of tubular organs and attachment apparatus has supported some functional interpretations (Mair et al 1998b(Mair et al , 2000Šebelova et al 2004;D'Ávila et al 2010).…”

“…The improvement of microscopy approaches have made possible great advance in studies of the morphology of digeneans (Robson & Erasmus 1970;Mair et al 1998aMair et al , b, 2000Abdul-Salam & Sreelatha 2000, 2004Stewart et al 2003;Halton 2004;Neves et al 2004Neves et al , 2005. Confocal scanning laser microscopy has become an important tool to clarify the organization of the musculature and innervation, as well as the morphology of the reproductive and alimentary tract of various species (Mair et al 1998a(Mair et al ,b, 2000Stewart et al 2003;Neves et al 2004Neves et al , 2005D'Ávila et al 2010;Machado-Silva et al 2011).…”