Gross and net nitrogen export from leaves of a vegetative C4 grass

Yang, Fang; Schäufele, Rudi; Liu, Hai Tao; Ostler, Ulrike; Schnyder, H.; Gong, Xiao Ying

doi:10.1016/j.jplph.2019.153093

Cited by 7 publications

(3 citation statements)

References 42 publications

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“…According to the mass balance, we have the expression of C mass (C mass ) of a mature leaf as a function of time-integrated influxes and effluxes: With I mp the import of carbon during the growth of the leaf. Applying isotopic mass balance we have, Given that the vast majority of the leaf structural biomass of grass species is inherited from its heterotrophic stage (Robinson-Beers, Sharkey & Evert 1990) and mature leaves of C. squarrosa have stopped elongation and their dry mass remained rather constant (Yang et al . 2020), C mass of a mature leaf should be close to the time-integrated I mp , implying that the rate of daily export in mature leaves equal its net assimilation: Combing Eqn 7 and 8, we have We define the isotopic fractionation of R ( e resp ) and export ( e exp ) relative to photosynthesis ( P ) of a mature leaf as (neglecting second-order terms): …”

Section: Methodsmentioning

confidence: 99%

“…Leaf Nr. 1 is the youngest leaf which included partially unexposed leaf blade tissue that was enclosed within the 'next-older leaves' sheath and represented the 'immature tissues' of a major tiller as described by (Yang et al 2020). Mature (that is fully expanded, green) leaves were numbered consecutively from the youngest (Nr.…”

Section: Plant Sampling and Isotopic Analysis For Bulk Dry Mattermentioning

confidence: 99%

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Leaf δ¹³C reveals post-photosynthetic fractionation during ontogeny in a C₄grass

Yu,

Liu,

Yang

et al. 2023

Preprint

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The13C isotope composition (δ13C) of leaf dry matter is a useful tool for physiological and ecological studies. However, how post-photosynthetic fractionation associated with respiration and carbon export influences δ13C remains uncertain. We investigated the effects of post-photosynthetic fractionation on δ13C of mature leaves ofCleistogenes squarrosa, a perennial C4grass, in controlled experiments with different levels of vapour pressure deficit and nitrogen supply. With the increase of leaf age classes, the12C/13C fractionation of leaf organic matter relative to the δ13C of atmosphere CO2(ΔDM) increased while that of cellulose (Δcel) was almost constant. The divergence between ΔDMand Δcelincreased with leaf age classes with a maximum value of 1.6‰, indicating the accumulation post-photosynthetic fractionation. Applying a new mass balance model that accounts for respiration and export of photosynthates, we found an apparent12C/13C fractionation associated with carbon export of –0.5 to –1.0‰. Different ΔDMamong leaves, pseudostems, daughter tillers and roots indicate that post-photosynthetic fractionation happens at the whole-plant level. Compared with ΔDMof old leaves, ΔDMof young leaves and Δcelare more reliable proxies for predicting physiological parameters due to the smaller sensitivity to post-photosynthetic fractionation and the similar sensitivity in responses to environmental changes.BRIEF SUMMARY STATEMENTΔ13C of bulk organic matter increases with leaf age classes while Δ13C of cellulose remain constant, lending support to the use of Δ13C of cellulose as a more reliable proxy for predicting physiological parameters due to the smaller sensitivity to post-photosynthetic fractionation.

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Section: Methodsmentioning

confidence: 99%

Section: Plant Sampling and Isotopic Analysis For Bulk Dry Mattermentioning

confidence: 99%

Leaf δ¹³C reveals post-photosynthetic fractionation during ontogeny in a C₄grass

Yu,

Liu,

Yang

et al. 2023

Preprint

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“…To see if xylem injection of 15 NH 4 Cl and K 15 NO 3 successfully produced 15 N-enriched seedlings, one fully developed leaf, representing a mature leaf at the age of 60 days, was collected from each seedling. Previous study has shown that the newly assimilated N and remobilized N from reserves have rather different 15 N signatures for a seedling (Yang et al, 2019;Cui et al, 2020). In addition, 15 N will be diluted in old leaves (Yi and Wang, 2015); we therefore selected mature leaves to create sub-samples for both the 15 N-enriched and control seedlings for isotope measurements, as N assimilation from soil and N remobilization from reserves are expected to reach a state of balance (Yi and Ju, 2020).…”

Section: Measurement Of 15 N Isotopes In Seedlingsmentioning

confidence: 99%

Tracking Animal-Dispersed Seedlings Using 15N Xylem Injection Method

Wang

et al. 2021

Front. Plant Sci.

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Although various seed-marking methods have been developed for seed dispersal, it remains difficult to track the actual patterns of seed dispersal and seedling recruitment. Thus, new labeling methods that accurately track seedling establishment along with seed movement would help us better understand seed dispersal. Here, we developed a new nondestructive method using 15N xylem injection to track seed dispersal and seedling recruitment based on the enriched isotopic signals in the mature seeds. Our results first showed that xylem injection of 15N successfully enriched 15N both in the acorns and seedlings of Quercus variabilis. By marking acorns and seedlings with 15N stable isotopes, we successfully tracked seedlings established from acorns dispersed by seed-eating animals in the field. Our xylem 15N injection caused little alteration to seeds and showed no significant effects on seed selection by seed-eating animals as well as seed germination and seedling establishment, verifying the validity of the 15N xylem injection method to track seedling establishment. Our xylem 15N injection method is expected to be a powerful tool for tracking seed dispersal and seedling recruitment mediated by seed-eating animals in seed dispersal ecology.

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Explorations into the Physiology and Ecology of Grassland Plants and Ecosystems: One Agronomist’s Academic Journey

Schnyder

2023

Progress in Botany

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Gross and net nitrogen export from leaves of a vegetative C4 grass

Cited by 7 publications

References 42 publications

Leaf δ¹³C reveals post-photosynthetic fractionation during ontogeny in a C₄grass

Leaf δ¹³C reveals post-photosynthetic fractionation during ontogeny in a C₄grass

Tracking Animal-Dispersed Seedlings Using 15N Xylem Injection Method

Explorations into the Physiology and Ecology of Grassland Plants and Ecosystems: One Agronomist’s Academic Journey

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Gross and net nitrogen export from leaves of a vegetative C4 grass

Cited by 7 publications

References 42 publications

Leaf δ13C reveals post-photosynthetic fractionation during ontogeny in a C4grass

Leaf δ13C reveals post-photosynthetic fractionation during ontogeny in a C4grass

Tracking Animal-Dispersed Seedlings Using 15N Xylem Injection Method

Explorations into the Physiology and Ecology of Grassland Plants and Ecosystems: One Agronomist’s Academic Journey

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Leaf δ¹³C reveals post-photosynthetic fractionation during ontogeny in a C₄grass

Leaf δ¹³C reveals post-photosynthetic fractionation during ontogeny in a C₄grass